Difference between revisions of "Team:BOKU-Vienna/Description"
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| − | <p><i>In vivo</i> continuous directed evolution offers significant advantages over classic <i>in vitro</i> methods as it drastically reduces the amount of time and actual lab work that needs to be invested. Most current approaches, however, are based on globally enhanced mutagenesis rates eventually leading to unwanted off-target mutations that interfere with the experiment. Here, we present the concept of a new continuous <i>in vivo</i> evolution strategy that allows complete spatial control of mutagenesis by cyclically using an RNA intermediate which finally replaces the original DNA cargo at the respective locus in the genome after it has been reverse-transcribed in an error prone way. </p> | + | <p> <br> <i>In vivo</i> continuous directed evolution offers significant advantages over classic <i>in vitro</i> methods as it drastically reduces the amount of time and actual lab work that needs to be invested. Most current approaches, however, are based on globally enhanced mutagenesis rates eventually leading to unwanted off-target mutations that interfere with the experiment. Here, we present the concept of a new continuous <i>in vivo</i> evolution strategy that allows complete spatial control of mutagenesis by cyclically using an RNA intermediate which finally replaces the original DNA cargo at the respective locus in the genome after it has been reverse-transcribed in an error prone way. </p> |
<p> Scroll down for more information.</p> | <p> Scroll down for more information.</p> | ||
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Revision as of 11:49, 25 June 2017
Project Overview
Description.
In vivo continuous directed evolution offers significant advantages over classic in vitro methods as it drastically reduces the amount of time and actual lab work that needs to be invested. Most current approaches, however, are based on globally enhanced mutagenesis rates eventually leading to unwanted off-target mutations that interfere with the experiment. Here, we present the concept of a new continuous in vivo evolution strategy that allows complete spatial control of mutagenesis by cyclically using an RNA intermediate which finally replaces the original DNA cargo at the respective locus in the genome after it has been reverse-transcribed in an error prone way.
Scroll down for more information.
Design.
In Progress ...
Experiments.
In Progress ...
Notebook.
In Progress ...
Results.
In Progress ...
