Difference between revisions of "Team:Lambert GA/Attributions"

Line 408: Line 408:
 
<p> <hh3> <b> Proof of Concept: </b> Natalie Shih, Alyssa Franklin </hh3> </p>
 
<p> <hh3> <b> Proof of Concept: </b> Natalie Shih, Alyssa Franklin </hh3> </p>
  
<h4>Chrom-Q</h4>
+
<h4>Chrome-Q</h4>
 
<p> David Standeven, Emily Gibson </hh3> </p>
 
<p> David Standeven, Emily Gibson </hh3> </p>
 
<h4> Wiki </h4>
 
<h4> Wiki </h4>
<p><hh3> <b> Design: </b> Neha Balachandran </hh3></p>
+
<p><hh3> <b> Design: </b> Nithik Balachandran, Kevin Li, Julia Leveille </hh3></p>
<p><hh3> <b> Design Support: </b> Sara Cleland, Jackson Harris, Arjun Bhatt </hh3></p>
+
<p><hh3> <b> Design Support: </b> Ellie Kim, Christina Lee, Noora Chandasir </hh3></p>
<p><hh3> <b> Information/Writing: </b> Arjun Bhatt, Jack Kwon, Natalie Shih, Nivi Minjur, Janet Standeven
+
<p><hh3> <b> Information/Writing: </b> Lambert's iGEM Team</hh3></p>
<p><hh3> <b> Pictures/Diagrams: </b> Arjun Bhatt
+
<p><hh3> <b> Pictures/Diagrams: </b> Lambert's iGEM Team</hh3></p>
  
 
<h4> Design </h4>
 
<h4> Design </h4>
<p> <hh3> <b> Spiritwear, Banner, Animations: </b> Elynna Chang </hh3></p>
+
<p> <hh3> <b> Spiritwear, Banner, Animations: </b> Ellie Kim </hh3></p>
<p> <hh3> <b> Poster: </b> Alyssa Franklin, Lauren Hong, Julia Leveille, Noora Chandasir</hh3> </p>
+
<p> <hh3> <b> Poster: </b> Noora Chandasir, Ellie Kim, Christina Lee</hh3> </p>
<p> <hh3> <b> Handouts: </b> Sara Cleland, Lubna Orberger </hh3></p>
+
<p> <hh3> <b> Handouts: </b> Ellie Kim, Christina Lee </hh3></p>
 +
 
 +
<h4> Ethics </h4>
 +
<p> <hh3> <b>Katie Barr, Kevin Li </hh3></p>
  
 
<h4> Outreach </h4>
 
<h4> Outreach </h4>

Revision as of 22:40, 29 October 2017


Attributions and Acknowledgements


Attributions

Research

Constructs: Lauren Hong, Julia Leveille, Katie Barr, Gaurav Byagathavalli, Ellie Kim, Alyssa Franklin

Measurement Model: David, Standeven, Emily Gibson, Nithik Balachandran

Labwork

Lauren Hong, Julia Leveille, Gaurav Byagathavalli, Katie Barr, Ellie Kim, Natalie Shih, Christina Lee, Kevin Li, Alyssa Franklin

Proof of Concept: Natalie Shih, Alyssa Franklin

Chrome-Q

David Standeven, Emily Gibson

Wiki

Design: Nithik Balachandran, Kevin Li, Julia Leveille

Design Support: Ellie Kim, Christina Lee, Noora Chandasir

Information/Writing: Lambert's iGEM Team

Pictures/Diagrams: Lambert's iGEM Team

Design

Spiritwear, Banner, Animations: Ellie Kim

Poster: Noora Chandasir, Ellie Kim, Christina Lee

Handouts: Ellie Kim, Christina Lee

Ethics

Katie Barr, Kevin Li

Outreach

Atlanta Science Festival: Noor Sohal, Elynna Chang, Neha Balachandran

Building with Biology: Nivi Minjur, Noora Chandasir, David Standeven, Natalie Shih

RESA: Noor Sohal, Neha Balachandran

Next Generation Focus: Neha Balachandran

CHD Walk: Noora Chandasir

Sharon Science Day: Sara Cleland

Presentation

Presenters: Arjun Bhatt, Neha Balachandran, Elynna Chang

Script/Other Help: Jack Kwon, Lauren Hong, Jackson Harris


Acknowledgements

General Support

Dr. Mark Styczynski: Associate Professor at Georgia Institute of Technology

Dr. Monica McNerney: Doctoral Student at Georgia Institute of Technology


In addition to providing resources, Monica McNerney and Dr. Styczynski assisted Lambert iGEM through its project by acting as mentors and aiding in the problem-solving conversations. There have been multiple conference calls in which Dr. Styczynski and Monica McNerney helped the Lambert iGEM team troubleshoot and revise its project. Dr. Styczynski has also generously provided materials that a high school lab does not have access to. We would like to thank them for helping us complete our project.

Dr. Anna Karls: Associate Professor at University of Georgia


Lambert iGEM collaborated with Dr. Karls, a microbiology professor at the University of Georgia. She gave feedback about how to assemble our construct more effectively.

Parts Support

Snapgene: Software for sequence analysis

IDT: Synthesis of g blocks

Funding/Materials Support

Lambert High School

National Science Foundation Grant #1254382

Siemens

AGCO

Franklin Technologies

Gofundme

Johns Creek Chick fil A

Rosco: Roscolux

Wiki Support

Joel Joseprabu: Wiki Formatting

Balachandran Srinivasan: Wiki Formatting

Outreach Support

Next Generation Focus/ Grace Chapel Hill Church

Atlanta Science Festival

Atlanta Maker Faire

Sharon Elementary School

Sources

And, S. A. (2009, February 13). Sarita Ahlawat. ClpXP Degrades SsrA-tagged proteins in S.pneumoniae.Retrieved Summer, 2017, from http://jb.asm.org/content/191/8/2894.full

Andersen , J.B. , Sternberg , C. , Poulsen , L.K. , Bjorn , S.P. , Givskov , M. , and Molin , S. ( 1998 ) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria . Appl Environ Microbiol 64 : 2240 – 2246 .

Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2017, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/

Bar-Nun, S., & Glickman, M. H. (2012). Proteasomal AAA-ATPases: Structure and function. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(1), 67–82. doi:10.1016/j.bbamcr.2011.07.009. Retrieved Summer, 2017 from http://www.sciencedirect.com/science/article/pii/S0167488911001984

Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 .

Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 .

Ciechanover, A. (2005). Cell death and differentiation - abstract of article: Intracellular protein degradation: From a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting[ast]. Cell Death & Differentiation, 12(9), 1178–1190. doi:10.1038/sj.cdd.4401692

Cooper, G. M. (2000). Protein degradation. Retrieved Summer, 2017 from http://www.ncbi.nlm.nih.gov/books/NBK9957/

Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2017, from http://www.colorcodepicker.com/

Farrell, C., Grossman, A., & Sauer, R. (2005). Cytoplasmic degradation of ssrA-tagged proteins.Molecular microbiology., 57(6), 1750–61. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16135238

Flynn , J.M. , Levchenko , I. , Seidel , M. , Wickner , S.H. , Sauer , R.T. , and Baker , T.A. ( 2001 ) Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis . Proc Natl Acad Sci USA 11 : 10584 – 10589.

Georgia Institute of Technology. (2015, September 1). “Bacterial litmus Test” provides inexpensive measurement of Micronutrients. Retrieved from GT News Center, http://www.news.gatech.edu/2015/09/01/bacterial-litmus-test-provides-inexpensive-measurement-micronutrients

Goldberg, A.L., A.S. Menon, S. Goff and D.T. Chin. 1987. The mechanism and regulation of the ATP-dependent protease La from Escherichia coli. Biochem. Soc. Trans. 15: 809-811. Retrieved October 1, 2017 from http://www.fao.org/wairdocs/ilri/x5550e/x5550e0d.htm

Hwang BJ, Woo KM, Goldberg AL, Chung CH. Protease Ti, a new ATP-dependent protease in Escherichia coli,contains protein-activated ATPase and proteolytic functions in distinct subunits. J Biol Chem. 1988;263:8727–8734.

Katayama-Fujimura Y, Gottesman S, Maurizi MR. A multiple-component, ATP-dependent protease from Escherichia coli. J Biol Chem. 1987;262:4477–4485.

Landry, B. P., & Stöckel, J. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Applied and Environmental Microbiology,79(8), 2833–2835. doi:10.1128/AEM.03741-12

Léa, D. (2017). Les meilleures banques en ligne - Banque en ligne de l'Université Cefi. Retrieved October 18, 2017, from http://univ-cefi.fr/les-meilleures-banques-en-ligne.html

Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-Dependent Proteases Degrade Their Substrates by Processively Unraveling Them from the Degradation Signal.

McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015). Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems. Metabolic Engineering, 31, 123–131. doi:10.1016/j.ymben.2015.06.011

Minikel, E. V. (2013, June 11). Basics of protein degradation. Retrieved Summer, 2017, from http://www.cureffi.org/2013/07/11/basics-of-protein-degradation/

Mogk A, Schmidt R, Bukau B. The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies. Trends Cell Biol. 2007;17:165–172.

Purcell, O., Grierson, C. S., Bernardo, M. di, & Savery, N. J. (2012). Temperature dependence of ssrA-tag mediated protein degradation. Journal of Biological Engineering, 6(1), . doi:10.1186/1754-1611-6-10

Purple color codes. (n.d.). Retrieved October 10, 2017, from http://www.rapidtables.com/web/color/purple-color.htm

RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2017, from http://www.perbang.dk/rgbgradient/

Schrader, E. K., Harstad, K. G., & Matouschek, A. (n.d.). Targeting proteins for degradation. , 5(11), . Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228941/

Snider, J., Thibault, G., & Houry, W. A. (2008). The AAA+ superfamily of functionally diverse proteins. , 9(4), . Retrieved Summer, 2017 from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643927/

Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x

Tanaka K. The proteasome: overview of structure and functions. Proc Jpn Acad Ser B Phys Biol Sci.2009;85:12–36.

Tao, L., & Biswas, I. (2015). Degradation of SsrA-tagged proteins in streptococci. , 161(Pt 4),. Retrieved September 9, 2017 from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857447/

Tu, D., Lee, J., Ozdere, T., Lee, T. J., & You, L. (2007, January ). Engineering Genetic Circuits: Foundations and Applications. Retrieved from http://people.duke.edu/~you/publications/Tu_etal_SyntheticBiology.pdf

Watstein, D. M., McNerney, M. P., & Styczynski, M. P. (2015). Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor. Metabolic Engineering,31, 171–180. doi:10.1016/j.ymben.2015.06.007






























































































Sponsors