Difference between revisions of "Team:Tianjin/Parts"

Line 222: Line 222:
 
<main id="main" class="about-main" role="main">
 
<main id="main" class="about-main" role="main">
 
             <article id="description">       
 
             <article id="description">       
−
                 <h1>Basic Part</h1>
+
                 <h1>Parts</h1>
 
                     <hr />
 
                     <hr />
  
Line 241: Line 241:
  
 
    
 
    
−
<h2>Helping TUST prepare for their first year of competition.</h2>
+
<h2>PARTS</h2>
−
<p>To begin with, we gave them construct suggestions, communicated with their team adviser. In 24/3/2017, we were invited to TUST to carried out recruiting propaganda for them, and provided our construction guide.</p>
+
<p>aaaaa</p>
  
  
 
<div id="parttable">   
 
<div id="parttable">   
−
 
+
<h3>Basic Parts</h3>
 
<table class="zebra">
 
<table class="zebra">
  
 
<thead>
 
<thead>
 
         <tr>
 
         <tr>
−
<th>Biobrick</th>
+
<th>Part number/name</th>
−
<th>Type</th>
+
<th>Classification</th>
 +
<th>length</th>
 
<th>Description</th>
 
<th>Description</th>
−
<th>Bp</th>
+
                <th>Blongings</th>
 
             </tr>
 
             </tr>
 
</thead>
 
</thead>
 
         <tbody>
 
         <tbody>
 +
        <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407000">BBa_K2407000</a></td>
 +
                <td>basic part</td>
 +
                <td>235bp</td>
 +
                <td>a Cu-induced promoter in Yeast</td>
 +
                <td>Biosenser</td>
 +
            </tr>
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407001">BBa_K2407001</a></td>
 +
                <td>basic part</td>
 +
                <td>442bp</td>
 +
                <td>a β-galactosidase-induced promoter switching on the expression of genes in the presence of galactose</td>
 +
                <td>SCRaMbLE</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407002">BBa_K2407002</a></td>
 +
                <td>basic part</td>
 +
                <td>417bp</td>
 +
                <td>a mitosis-specific promoter</td>
 +
                <td>SCRaMbLE</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407003">BBa_K2407003</a></td>
 +
                <td>basic part</td>
 +
                <td>248bp</td>
 +
                <td>a terminator used in yeast</td>
 +
                <td>SCRaMbLE</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407004">BBa_K2407004</a></td>
 +
                <td>basic part</td>
 +
                <td>4614bp</td>
 +
                <td>trigger the yeast flocculation</td>
 +
                <td>SCRaMbLE</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407005">BBa_K2407005</a></td>
 +
                <td>basic part</td>
 +
                <td>989bp</td>
 +
                <td>specific identification and react with loxP loci</td>
 +
                <td>SCRaMbLE</td>
 +
            </tr>
 
         <tr>
 
         <tr>
−
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407103">BBa_K2407103</a></td>
+
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407012">BBa_K2407012</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
−
                 <td>produce L-Isoleucine to live on</td>
+
                 <td>711bp</td>
−
                 <td>100</td>
+
                <td>a modified yEmRFP for use in Yeast from mCherry mRFP</td>
 +
                <td>Biosenser & Characterization</td>
 +
            </tr>
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407013">BBa_K2407013</a></td>
 +
                <td>basic part</td>
 +
                <td>235bp</td>
 +
                <td>a modified CUP1 promoter obtained by Error-Prone PCR EP3</td>
 +
                <td>Biosenser</td>
 +
            </tr>
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407014">BBa_K2407014</a></td>
 +
                <td>basic part</td>
 +
                <td>234bp</td>
 +
                <td>a modified CUP1 promoter obtained by Error-Prone PCR EP5</td>
 +
                <td>Biosenser</td>
 +
            </tr>
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407015">BBa_K2407015</a></td>
 +
                <td>basic part</td>
 +
                <td>235bp</td>
 +
                <td>a modified CUP1 promoter obtained by Error-Prone PCR EP9</td>
 +
                <td>Biosenser</td>
 +
            </tr>
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407016">BBa_K2407016</a></td>
 +
                <td>basic part</td>
 +
                <td>235bp</td>
 +
                <td>a modified CUP1 promoter obtained by Error-Prone PCR EP28</td>
 +
                 <td>Biosenser</td>
 
             </tr>
 
             </tr>
 
             <tr>
 
             <tr>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407104">BBa_K2407104</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407104">BBa_K2407104</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
 +
                <td>703bp</td>
 
                 <td>a Modified-Gal inducable promoter working with the TF Z4EV</td>
 
                 <td>a Modified-Gal inducable promoter working with the TF Z4EV</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
 
             </tr>
 
             </tr>
 
             <tr>
 
             <tr>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407105">BBa_K2407105</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407105">BBa_K2407105</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
 +
                <td>1499bp</td>
 
                 <td>a transcription factor working with Modified-Gal promoter</td>
 
                 <td>a transcription factor working with Modified-Gal promoter</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
 
             </tr>
 
             </tr>
 
             <tr>
 
             <tr>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407106">BBa_K2407106</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407106">BBa_K2407106</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
 +
                <td>1072bp</td>
 
                 <td>a constitutive promoter</td>
 
                 <td>a constitutive promoter</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
 
             </tr>
 
             </tr>
 
             <tr>
 
             <tr>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407107">BBa_K2407107</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407107">BBa_K2407107</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
 +
                <td>1295bp</td>
 
                 <td>a constitutive promoter</td>
 
                 <td>a constitutive promoter</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
−
             </tr>
+
             </tr><tr>
−
            <tr>
+
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407108">BBa_K2407108</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407108">BBa_K2407108</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
 +
                <td>236bp</td>
 
                 <td>a terminator used in yeast</td>
 
                 <td>a terminator used in yeast</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
−
             </tr>
+
             </tr><tr>
−
            <tr>
+
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407109">BBa_K2407109</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407109">BBa_K2407109</a></td>
 
                 <td>basic part</td>
 
                 <td>basic part</td>
 +
                <td>248bp</td>
 
                 <td>a terminator used in yeast</td>
 
                 <td>a terminator used in yeast</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407111">BBa_K2407111</a></td>
 +
                <td>basic part</td>
 +
                <td>186bp</td>
 +
                <td>express a metallothionein to enrich copper ions in yeast</td>
 +
                <td>Enrichment for Heavy Metals</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407112">BBa_K2407112</a></td>
 +
                <td>basic part</td>
 +
                <td>319bp</td>
 +
                <td>express a metallothionein to enrich cadmium ions in yeast</td>
 +
                <td>Enrichment for Heavy Metals</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407300">BBa_K2407300</a></td>
 +
                <td>basic part</td>
 +
                <td>408bp</td>
 +
                <td>A strong promotor in Yeast</td>
 +
                <td>Characterization</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407301">BBa_K2407301</a></td>
 +
                <td>basic part</td>
 +
                <td>804bp</td>
 +
                <td>Ura3</td>
 +
                <td>Characterization</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407302">BBa_K2407302</a></td>
 +
                <td>basic part</td>
 +
                <td>78bp</td>
 +
                <td>Ura3 terminator</td>
 +
                <td>Characterization</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407304">BBa_K2407304</a></td>
 +
                <td>basic part</td>
 +
                <td>328bp</td>
 +
                <td>ADH1 yeast terminator</td>
 +
                <td>Characterization</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407305">BBa_K2407305</a></td>
 +
                <td>basic part</td>
 +
                <td>673bp</td>
 +
                <td>TDH3 yeast Promoter</td>
 +
                <td>Characterization</td>
 
             </tr>
 
             </tr>
 +
  </tbody>
 +
</table>
 +
   
 +
<h3>Composite Part</h3> 
 +
 
 +
    <table class="zebra">
 +
 +
<thead>
 +
        <tr>
 +
<th>Part number/name</th>
 +
<th>Classification</th>
 +
<th>length</th>
 +
<th>Description</th>
 +
                <th>Blongings</th>
 +
            </tr>
 +
</thead>
 +
        <tbody>   
 
             <tr>
 
             <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407100">BBa_K2407100</a></td>
 +
                <td>composite part</td>
 +
                <td>2453bp</td>
 +
                <td>a composite part to control the expression of HO gene</td>
 +
                <td>MTS</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407101">BBa_K2407101</a></td>
 +
                <td>composite part</td>
 +
                <td>2571bp</td>
 +
                <td>express the transcription factor Z4EV</td>
 +
                <td>MTS</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407102">BBa_K2407102</a></td>
 +
                <td>composite part</td>
 +
                <td>2020bp</td>
 +
                <td>control the expression of vika gene</td>
 +
                <td>MTS</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407103">BBa_K2407103</a></td>
 +
                <td>composite part</td>
 +
                <td>2570bp</td>
 +
                <td>produce L-Isoleucine to live on</td>
 +
                <td>MTS</td>
 +
            </tr><tr>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407110">BBa_K2407110</a></td>
 
             <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407110">BBa_K2407110</a></td>
−
                 <td>basic part</td>
+
                 <td>composite part</td>
 +
                <td>1086bp</td>
 
                 <td>express the vika recombinase as a part in a novel site-specific recombination system</td>
 
                 <td>express the vika recombinase as a part in a novel site-specific recombination system</td>
−
                 <td>100</td>
+
                 <td>MTS</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407303">BBa_K2407303</a></td>
 +
                <td>composite part</td>
 +
                <td>6649bp</td>
 +
                <td>a composite part of three key genes to produce β-carotene</td>
 +
                <td>Characterization</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407306">BBa_K2407306</a></td>
 +
                <td>composite part</td>
 +
                <td>8045bp</td>
 +
                <td>a composite part react with Vika recombinase</td>
 +
                <td>Characterization</td>
 +
            </tr><tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407307">BBa_K2407307</a></td>
 +
                <td>composite part</td>
 +
                <td>8310bp</td>
 +
                <td>a composite part react with Vika recombinase</td>
 +
                <td>Characterization</td>
 
             </tr>
 
             </tr>
 +
                    </tbody>
 +
</table>
 +
<h3>Device part</h3>
 +
           
 +
  <table class="zebra">
 +
 +
<thead>
 +
        <tr>
 +
<th>Part number/name</th>
 +
<th>Classification</th>
 +
<th>length</th>
 +
<th>Description</th>
 +
                <th>Blongings</th>
 +
            </tr>
 +
</thead>
 +
        <tbody>       
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407010">BBa_K2407010</a></td>
 +
                <td>device part</td>
 +
                <td>5304bp</td>
 +
                <td>a device to control the flocculation of yeast</td>
 +
                <td>MTS</td>
 +
            </tr>
 +
            <tr>
 +
            <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2407011">BBa_K2407011</a></td>
 +
                <td>device part</td>
 +
                <td>3407bp</td>
 +
                <td>a device to switch Cre-loxP recombination system on and off</td>
 +
                <td>MTS</td>
 +
            </tr> 
 
         </tbody>
 
         </tbody>
 
</table>
 
</table>

Revision as of 06:49, 30 October 2017

/* OVERRIDE IGEM SETTINGS */

Parts

PARTS

aaaaa

Basic Parts

Part number/name Classification length Description Blongings
BBa_K2407000 basic part 235bp a Cu-induced promoter in Yeast Biosenser
BBa_K2407001 basic part 442bp a β-galactosidase-induced promoter switching on the expression of genes in the presence of galactose SCRaMbLE
BBa_K2407002 basic part 417bp a mitosis-specific promoter SCRaMbLE
BBa_K2407003 basic part 248bp a terminator used in yeast SCRaMbLE
BBa_K2407004 basic part 4614bp trigger the yeast flocculation SCRaMbLE
BBa_K2407005 basic part 989bp specific identification and react with loxP loci SCRaMbLE
BBa_K2407012 basic part 711bp a modified yEmRFP for use in Yeast from mCherry mRFP Biosenser & Characterization
BBa_K2407013 basic part 235bp a modified CUP1 promoter obtained by Error-Prone PCR EP3 Biosenser
BBa_K2407014 basic part 234bp a modified CUP1 promoter obtained by Error-Prone PCR EP5 Biosenser
BBa_K2407015 basic part 235bp a modified CUP1 promoter obtained by Error-Prone PCR EP9 Biosenser
BBa_K2407016 basic part 235bp a modified CUP1 promoter obtained by Error-Prone PCR EP28 Biosenser
BBa_K2407104 basic part 703bp a Modified-Gal inducable promoter working with the TF Z4EV MTS
BBa_K2407105 basic part 1499bp a transcription factor working with Modified-Gal promoter MTS
BBa_K2407106 basic part 1072bp a constitutive promoter MTS
BBa_K2407107 basic part 1295bp a constitutive promoter MTS
BBa_K2407108 basic part 236bp a terminator used in yeast MTS
BBa_K2407109 basic part 248bp a terminator used in yeast MTS
BBa_K2407111 basic part 186bp express a metallothionein to enrich copper ions in yeast Enrichment for Heavy Metals
BBa_K2407112 basic part 319bp express a metallothionein to enrich cadmium ions in yeast Enrichment for Heavy Metals
BBa_K2407300 basic part 408bp A strong promotor in Yeast Characterization
BBa_K2407301 basic part 804bp Ura3 Characterization
BBa_K2407302 basic part 78bp Ura3 terminator Characterization
BBa_K2407304 basic part 328bp ADH1 yeast terminator Characterization
BBa_K2407305 basic part 673bp TDH3 yeast Promoter Characterization

Composite Part

Part number/name Classification length Description Blongings
BBa_K2407100 composite part 2453bp a composite part to control the expression of HO gene MTS
BBa_K2407101 composite part 2571bp express the transcription factor Z4EV MTS
BBa_K2407102 composite part 2020bp control the expression of vika gene MTS
BBa_K2407103 composite part 2570bp produce L-Isoleucine to live on MTS
BBa_K2407110 composite part 1086bp express the vika recombinase as a part in a novel site-specific recombination system MTS
BBa_K2407303 composite part 6649bp a composite part of three key genes to produce β-carotene Characterization
BBa_K2407306 composite part 8045bp a composite part react with Vika recombinase Characterization
BBa_K2407307 composite part 8310bp a composite part react with Vika recombinase Characterization

Device part

Part number/name Classification length Description Blongings
BBa_K2407010 device part 5304bp a device to control the flocculation of yeast MTS
BBa_K2407011 device part 3407bp a device to switch Cre-loxP recombination system on and off MTS

2017.08.01

In 2017.08.01, TUST and Tianjin held a meeting together, during the meeting, TUST gave an account of their recent progress, we suggested them to add some new synthetic routes and elements, and probably new strain instead of simply Xylinus for fibrin.

Later on

Later on, TUST came again with their modeling problem, we gave them suggestion that we can share the Fluorescence method and modeling method with them, design Fluorescence experience and modeling together since it’s their first year competition.

Helping NKU with their plasmid construction

1. NKU asked us to help them construct a plasmid. Using pEX18Gm as the supporter, we ought to added Lyase gene sequence（which they had already provided） and xyR-xyA sequence from pAX01 as well.

After construction

After construction, they sent us plasmid pAX01 and the plasmid which contains Lyase gene sequence, we helped them with ligasion and transformation.

Test the mini system for OUC

Mini system is a system composed of a mini promoter (about 100bp) and a mini terminator, and it is applicable for yeast. Because of the similarity of our biological chassis, they reached out to us and wanted us to run some control experiments for them. Using the same protocol and their mini system, we need to transform the system into our Saccharomyces cerevisiae in order to test if the plasmid will still function the same.

Protocol

2. Protocol: initial OD600 = 0.01 Test OD600 and threshold fluorescence every other 5-6 hours Exciting light λ=502/Emissive light λ=532

Sent a Collaboration Request and construct an alliance to build a worldwide database.

We came up with the idea that we could gather all the iGEM team whose project is about water pollution treatment together and build an alliance to unite all our social impact, knowledge and geographical advantages.

2017.08.12

2. In 12th of August, we had a voice conferencing with SJTU/SCUT/XMU/UCAS/JLU/FAFU, we discussed about how we want to use this alliance and came up with 2 conclusion: 1. Build a worldwide database containing contents of heavy metals in soil 2. Mutual improve our social impact

2017.08.23

In 23th of August, we sent a Collaboration Request to iGEM official website, the next day Ana Sifuentes replied our message and posted our request in the iGEM official website, and we received the response of many teams like team EXETER and team CSMU NCHU TAIWAN, with their information, we construct a database which contains the worldwide data of contents of Cu2+/Cd2+ in soil or water. And we build it based on map of the world.

Helping CQU construct their team and sign up for their first year

First, I believe that this nation should commit itself to achieving the goal, before this decade is out, of landing a man on the moon and returning him safely to the earth. No single space project in this period will be more impressive to mankind, or more important for the long-range exploration of space; and none will be so difficult or expensive to accomplish.

This one goes deeper

A good rule for rocket experimenters to follow is this: always assume that it will explode. The vehicle explodes, literally explodes, off the pad. The simulator shakes you a little bit, but the actual liftoff shakes your entire body and soul.

Even deeper

The path of the righteous man is beset on all sides by the iniquities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of darkness, for he is truly his brother's keeper and the finder of lost children.

And a level-3 title again

It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.

Filmed a biosafety video together with other 12 teams

12 teams gather together to film a biosafety video, every team took different topics, but all based on Yale biosafety manual.

This one goes deeper

A good rule for rocket experimenters to follow is this: always assume that it will explode. The vehicle explodes, literally explodes, off the pad. The simulator shakes you a little bit, but the actual liftoff shakes your entire body and soul.

Even deeper

The path of the righteous man is beset on all sides by the iniquities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of darkness, for he is truly his brother's keeper and the finder of lost children.

And a level-3 title again

It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.