Basic Parts

Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.

Registry link	Part	Plasmid	Summary	Length
BBa_K2404000	TSH	pSB1C3	A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification	780bp
BBa_K2404001	TSHH	pSB1C3	A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification	832bp
BBa_K2404002	PDF1.2	pSB1C3	An inducible promoter that responds to the presence of jasmonic acid in the cell	522bp
BBa_K2404003	PR2	pSB1C3	An inducible promoter that responds to the presence of salicylic acid in the cell	519bp
BBa_K2404004	GST6	pSB1C3	An inducible promoter that responds to the presence of salicylic acid in the cell	519bp
BBa_K2404005	WRKY30	pSB1C3	An inducible promoter that responds to the presence of cellulose-derived oligomers	507bp
BBa_K2404006	Luc+	pSB1C3	A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression	1653bp
BBa_P10401	NosT	pSB1C3	A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37	285bp
BBa_P10000	LexA	pSB1C3	An estradiol inducible promoter from the LexA gene which is native to Escherichia coli	362bp
BBa_P10003	35S-OTMV	pSB1C3	A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus	1446bp