Difference between revisions of "Team:Fudan China/MSS"

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         <h1>Description</h1>
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         <h1>Multi-signal system</h1>
 
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Revision as of 18:03, 30 October 2017

Design of the system

Of course, recording one changing signal in only two time periods is not enough for us to solve real-world problems. As a result, we have further designed multi-signal system based on two-signal system, which records one changing signal in more than two time periods.

Overview of the whole circuit

Like two-signal system, multi-signal system is composed of three parts, logic gates, sequential memory structures and basic orthogonal memory modules(Fig.1). The main difference between two systems is the sequential memory structures. Due to the diversity and orthogonality of serine integrases, much more orthogonal integrases used in sequential memory structures and basic orthogonal memory modules can be involved in this extending system.

Sequential memory structures

Fig.2 shows the sequential memory structures of multi-signal system. Not mentioned before, attB/P sites of integrases have core sequences. By mutating these sequences, orthogonal sites of one integrase can be made, which means that one integrase may control inversion of two sites, and that there can be little cross-talk. It helps to design this part,

Conclusion