Difference between revisions of "Team:UESTC-China"

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<p id='p1'>Haloalkanes are widely used commercially. The majority of these compounds have been shown to be serious pollutants as they are toxic and quite persistent in the environment, such as a man-made industrial chemical 1,2,3-Trichloropropane (TCP). The TCP has been introduced into our environment as a consequence of industrial waste disposal and widespread open use in agriculture, which need to be removed to low levels from waste streams and during sanitation of polluted sites. For this reason, we hope to use the plant root system to express three key enzymes: DhaA31, HheC, EchA, to degrade the TCP in the soil and sewage, and to realize the friendly repair of the environment.</p>
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<p id='p2'>DhaA from Rhodococcus sp can hydrolyze TCP to the corresponding haloalcohol. DhaA31 is the mutant of DhaA which has better degradation capacity. Haloalcohol dehalogenase HheC which comes from Agrobacterium radiobacter AD1 is a potentially useful enzyme involved in the degradation of several important environmental pollutants,  such as 1,3-dichloro-2-propanol, 2,3-dichloropropa-nol, 1-chloropropanol, epichlorohydrin and so on. EchA, epoxide hydrolase, also comes from Agrobacterium radiobacter AD1 , catalyzing various epoxide and aromatic oxide. Using these three enzymes, we constructed a TCP degradable metabolic pathway, which can eventually degrade the pollutant TCP to harmless glycerol.
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Revision as of 23:43, 30 October 2017

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