Difference between revisions of "Team:UCLouvain/Team"
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Revision as of 01:25, 31 October 2017
Using the iGEM’s biobricks, we construct a plasmid containing a gene coding for a RFP. Arabinose activates this gene, thanks to its pBAD promoter.
On the other hand, we knocked out (KO) a gene required for tyrosine synthesis in E. coli, TyrA. A medium containing photo-caged tyrosine (Ortho Nitro Benzyl-Tyrosine) is used to grow the bacteria: this special tyrosine is sensitive to UV’s, and will be only available for our KO E. coli once uncaged.
learn moreComS is a small peptide imported into the cytoplasm by the cell. Interacting with ComR, it will form a complex, that acts as a gene activator.
ComS contains some tyrosine. Thanks to a collaboration with…, we synthetize ComS using the photo-caged Tyrosine instead of Tyrosine. This new ComS can’t interact with ComR, unless its Tyrosine have been uncaged by UV’s.
The complex of ComR/ComS will simply activate a RFP gene, creating as previously a system responding to UV’s.
learn morePatrice Soumillion is teaching biochemistry and enzymology at the Université catholique de Louvain. He is a specialist in the functioning and evolution of enzymes. His research team is part of the group of biochemistry, biophysics and genetics of microorganisms at the Life Sciences Institute. With his help and expertise, he follows us all along our scientific adventure and he gives us valuable advice.
Charles Hachez is teaching at the Faculty of Sciences of the Université catholique de Louvain, Belgium. He is specifically interested in the molecular genetic structure ruling the development of glandular trichomes tractable in the leaf epidermis of Nicotiana tabacum. He will accompany our team during the Giant Jamboree in Boston.
Bernard Hallet is also a professor at the Faculty of Sciences of the Université catholique de Louvain, Belgium. He conducts research on the molecular system that mediate specialized DNA rearrangements in bacteria. As Prof. Hachez and Prof. Soumillion, he has been an important actor in our project. Thanks to his support and knowledge, we are succeeding to develop BactaSun
