Revision as of 04:50, 31 October 2017

Notebook

Week 1 (March 19-25)

Consulted with our advisor, Frank, to advise researchers and scientists
Reached out to scientists at Mt Sinai Hospital, Jason Fuller and Russell Hanson
Met with Jason Fuller and Russell Hanson, scientists at Genetics Department at Mt Sinai Hospital
Scheduled weekly meetings for the rest of the year

Week 2 (March 26-Apr 1)

Brainstormed for a potential topic (Sickle Cell Anemia, Cystic Fibrosis, Huntington’s Disease); decided on HD, proposed by Yeji Cho
Determined project leaders of the project
Divided into subsections (website, design, fundraising, social media, computational) and assigned roles, such as other team leads

Week 3 (Apr 2-8)

Determined possible method to target the disease
Outlined the abstract for the project

Week 4 (Apr 9-15)

Took headshots of members and team pictures to put on website
Administered Drug Tests for medical clearance
HIPAA (Health Insurance Portability and Accountability Act) Training
Fire Drill Practice

Week 5 (Apr 16-22)

Decided to use WordPress as website template
Happily received our lab coats and Mount Sinai IDs

Week 6 (Apr 23-29)

Submitted payment for iGEM group registration
Signed consent forms

Week 7 (Apr 30-May 6)

Designed graphic of brain and neuron for website

Week 8 (May 7-13)

Brainstormed for a team name and designed logo; decided on HD Resolution

Week 9 (May 14-20)

Designed T-shirts, hats, and sweaters
Brainstormed for companies/sponsors to partner with us
Received iGEM parts kit

Week 10 (May 21-27)

Organized home page of website
Created sponsorship/partnership letters for possible companies

Week 11 (May 28-Jun 3)

Julia, Erik and Stacy organized a table at Peace Angels Benefit Concert at Steinway and Sons Concert Hall in May 28, 2017
Mailed out consent forms
Scheduled wetlab training dates for the summer

Week 12 (Jun 4-10)

Early Training: lab safety, project overview, practiced lab techniques such as pipetting, centrifugation, PCR, etc.

Week 13 (Jun 11-17)

Organized team page of website
Contacted over 50 private and public companies in pharmaceutical and biotech industries, non-profit organizations for sponsorship and support.

Week 14 (Jun 18-24)

Created Twitter account
Christi and Marianne completed safety form 1, 2, 3

Week 15 (Jun 25-Jul 1)

Jason, Justin, Jeewhan, Marianne met up to analyze the data collection

Week 16 (Jul 2-8)

Computational Lab Weeks:
First weeks we talked about how to get an application called mFold
mFold helps to create a simulation of the RNA bonds fusing instead having to actual do it experimentally

Week 17 (Jul 9-15)

Computational Lab Weeks (continued):
Looked for Huntington’s mRNA in particular in NCBI
NM numbers, other prefixes gave types of mRNA
HW: Find huntington’s-related sequences in NCBI database (assumed at least 5-10 sequences)

Week 18 (Jul 16-22)

Christi, Rachel, Esther, and Marianne organized a fundraising and information table at Dominico-American Society of Queens in Flushing Town Hall in Flushing, Queens NY in July 22 2017
Created Facebook account
Created GoFundMe page

Week 19 (Jul 23-29)

Received over 100 likes on Facebook page thanks to our Social Media and Outreach team.
Computational Data:
We used data we received from [ncbi.com] and used it to compare with the wild type and the infected types.
HW Results: Found 2 accession numbers- NM_002111.8 (mRNA) and NP_002102.4 (protein); however, only one was mRNA and none were disease form
We then collected the data in our shared folder
Looked through other databases for other mutants; found:
Study with “20 Huntington’s Disease and 49 neurologically normal control samples from post-mortem human subjects”
http://trace.ddbj.nig.ac.jp/DRASearch/study?acc=SRP051844
Results for HTT on DNA Data Bank of Japan (includes above):
http://trace.ddbj.nig.ac.jp/DRASearch/query?keyword=htt&show=20&fq_rep_name=Homo%20sapiens
HW: Run wild-type mRNA on mfold (Create a text document with the NM_00211.8 RNA and run mFold on it http://unafold.rna.albany.edu/?q=mfold)
We then collected the data in our shared folder

Week 20 (Jul 30-Aug 5)

Ordered materials (E. coli, etc)
Computational Data:
HW Results: Nobody able to run mfold successfully locally or on web
Changed programs to ViennaRNA:

HW: Re-calculate sequences with artificially added CAG repeats (added 42 and 60 repeats)

Week 21 (Aug 6-12)

Wrote letters to respective schools to ask for partnerships
Manually analyzed results of ViennaRNA calculations for chaperone/guideRNA hairpin targets.
Determined that this process was less reproducible than desired, and it worried the most viable hairpin loop might be buried in the the tertiary structure
Decided to change approach: Use RNAi tools to calculate the toe-hold footing (areas in RNA that can be used to initiate strand displacement and/or break apart mRNA secondary structures).
Spent remainder of the day testing the following tools:
We used to addgene.com to find plasmids that had less than 26Q repeats.
We used a genome to protein sequence converter called [] in order to compare the wild type plasmid and the infected.
At first we thought we had a good candidate with 20Q that was very similar, however, didn’t work because the sequence didn’t completely match up with the wild type.
Therefore kept looking and decided to use pEGFP-Q23 https://www.addgene.org/40261/

Week 22 (Aug 13-19)

Created Instagram account
Grew cell subcultures of E. Coli for future testing
Familiarized ourselves with autoclaving and basic sterilizing processes
Learned how to store E. coli for future experiments
HDSA agreed to be sponsor

Week 23 (Aug 20-26)

Learned about transfection and tested cells for competency
Updated team Wiki’s page on the iGem website
Reached out to our respective high schools for sponsorship
Hereditary Disease Foundation/Huntington’s Disease Foundation agreed to be sponsor

Week 24 (Aug 27-Sept 2)

Jessica, Erin, Catherine, and Yunsu helped organize a presentation table at Dominico-American Society of Queens Benefit Concert at National Opera America Center in New York NY on August 30 2017
Reached out to Cerebral Palsy Alliance Research Foundation about partnering and sponsorship and discussed creating a joint campaign CPARF’s Steptember campaign to promote 10,000 steps a day goal
Registered team members and created a team roster

Testing Competency of Cells

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-19

Tuesday, 9/19/17

1.

Clean working area with 10% ethanol

2.

Throw competent cells on ice

3.

Label one 1.5 mL microcentrifuge tubes for each transformation and pre-chill on ice.

a.

Orange = 50 pg/μl

b.

Yellow = 10 pg/μl

c.

Red = 100 pg/μl

4.

Spin down DNA tubes from competent cell test kit. Quick spin of 20-30 seconds at 8000-10000rpm

5.

Pipet 10μl of DNA into centrifuge tubes. 50μl of competent cells already aliquoted. Flick gentlly

6.

Incubate on ice for 30 min.

7.

Heatshock cells into water bath of 42 degrees Celsius for 45 sec.

8.

Immediately transfer tubes back into ice and incubate on ice for 5 min

9.

Add 950 μl of SOC Media per tube and incubate at 37 degrees Celsius for 1 hour, shaking at 200-300rpm.

10.

Pipet 100μl from each tube onto appropriate plate, spread mixture evenly.

11.

Incubate at 37 degrees Celsius overnight/16 hrs. Place plates with agar side on top and lid on bottom.

Untitled

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-20

Wednesday, 9/20/17

1.

Innoculated 600 mL LB w/ 1 mL O/N culture

2.

Shake at 37 degrees Celsius, DDC 600 = 0.4, 3 hr

3.

Cooled all materials in ice, including solutions (CaCl3), cells, tubes, & tips

4.

Aliquoted 600 mL of cells into 12 50 mL tubes, 30 mL each

5.

Spin 1.5 k rcf, 5 min

6.

Decant sup, resus, pellets in 1 mL CaCl2 (cold) by gentle rocking, left on ice for 30 min

7.

Spin as above

8.

Discard supernatent

9.

Added fresh cold 100 mM CaCl3

10.

Resuspend as above

11.

Added 10.2 mL 50% glycerol to 50 mL falcon tube (chilled)

12.

Consolidated all resuspended cells into the tube containing 50% glycerol

13.

Did not fully resuspend cells, still looked chunky

14.

Froze in 1 mL aliquots of liiquid nitrogen, left in unmarked box below iGEM box in -80 degrees Celsius freezer

Continuation

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-22

Friday, 9/22/17

1.

Added 175μl of ice cold Alkaline Lysis Solution III through the viscous bacterial lysate. Inverted tube several times and stored tube on ice for 5 min.

2.

Centrifuged lysate at maximum speed for 5 min at 4 degrees Celsius, and transferrred the supernatant to a new tube

3.

Precipitated the nucleic acids from the supernatant. Added 1 mL of ethanol at room temperature. Mixedsolution by vortexing and allowed mixture to stand for two minutes at room temp.

4.

Collected precipitated nucleic acds by centrifugation at max speed for 5 min at 4 degrees Celsius. Dropped precipitated nucleic aicds. Redid centrifugation at max spped for 5 minutes at 4 degrees Celsius.

5.

Removed supernatant by gentle aspiration, as shown in step 4. Stood tube in an inverted position on a paper towel to allow all the fluid to drain away. Used a kimwipe to remove the drops of fluid adhering to the walls of the tube.

6.

Added 1 mL of 70% ethanol to the pellet and inverted the closed tube several times.

7.

Recovered DNA by centrifugation at max speed for 2 min at 4 degrees Celsius in a microcentrifuge.

Tranformation

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-25

Monday, 9/25/17

1.

Thaw DHS-Alpa Cells on ice

2.

Add 2 mL of DNA to cells (from previously isolated adgene plasmids)

3.

Stand for 34 min on ice

4.

Cells are incubated for 60 sec at 42 degrees Celsius

5.

Put cells on ice for 5 minut s

6.

Add 900μl SOC media

Preparation of LB-Agar Plates

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-26

Tuesday, 9/26/17

Preparation of LB-Agar Plates

- Adapted from CSH Protocols

LB Agar

- 3.75 gramf of Agar

- 250 mL of 1x LB Medium

1.

Mix

2.

Autoclave on Liquid Cycle for 30 minutes

a.

Do not overfill bottles

I.

250 mL of LB was autoclaved in a 500 mL bottle to prevent it from boiling over.

3.

Add 2.5 mL of Kanamycin Stock (25 mg/mL) to a final concentration of 25ug/mL, once LB-Agar has coded to about 50 degrees Celsius.

4.

Heat on a hot plate to re-melt sugar

Running Electrophoresis Gel of ADDGENE Plasmid Digests

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-26

Tuesday, 9/26/17

1.

Cut up unused gel and add to remaining unused 2% agarose state.

2.

Warm until gel molds / 2.5 for 5 min

3.

Pour into casting pan

Interlab & Second Competent Cell Test

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-26

Tuesday, 9/26/17

1.

Clean working area

2.

Thaw competent cells on ice

3.

Label 8 microcentrifuge tubes : 10ug, 50ug, 100ug, 1, 4, 5, 6, Neg.

4.

Spin down DNA from competent cell test kit and interlab DNA

5.

Pipet 1μl of DN from competent cell test kit into tubes labeled 1, 4, 5, 6, neg

6.

Pipet 85μl of competent cells into all tubes, flick gently to mix

7.

Incubate on ice for 30 minutes

8.

Heat shock cells by placing tubes into a hot water bath at 42 degrees Celsius for 45 seconds

9.

Transfer back and incubate on ice for 5 min

10.

Add 45μl of SOC Media into eah cand incubate at 37 degrees for 1 hour shaking at 200-300 rpm

11.

Warm up agar plates, label

12.

Pipet 100μl from each tube onto appropriate plate, spread mixture evenly across plates via sterile glass beads.

13.

Close with parafilm

14.

Incubate at 37 degrees Celsius overnight w/ plates positioned w/ agar side at top and lid at bottom

Interlab

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-09-27

Wednesday, 9/27/17

1.

Clean working area

2.

Pipette 1μl of 1, 4, 5, 6, neg - and control each with their respective cell tube

3.

INcubate for 30 min (until 7:02)

4.

Pipette 100μl of LUDOX into wells A1, B1, C1. D1 each

5.

Pipette 100μl of H2O each into wells A2, B2, C2, D2

6.

Spin down fluorescein tube

7.

Resuspend 100μM florescein solution in 1 mL 1xPBS to make 2x solution

8.

Dilute to 1x.

9.

Perform serial dilution with 10 columns of 1x PBS (leave an additional column as regular PBS)

Restriction Digest on Previously Isolated DNA Plasmid

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-10-06

Friday, 10/6/17

1.

Placed previously isolated DNA plasmid on ie (61 and 62)

2.

Measured the concentration of the DNA Plasmids with the nanodrop (2μl)

a.

DNA plasmid 40261 : 356.2 ng/μl

b.

DNA plasmid 40262 : 300.5 ng/μl

3.

Diluted DNA plasmid 1 : 3 to approximate 100 ng/μl and measured with nanodrop

a.

DNA plasmid 40261 : 117.9 ng/μl

b.

DNA plasmid 40262 : 106.1 ng/μl

4.

Labeled fresh 0.5 mL tubes 61 and 62

5.

For each tube, transferred 5 ul of 10x NEBuffer, 1 ul of Stul, 1 mL of cac 81, and 33 ul of dH2O

6.

Transferred 10μl DNA Plasmid 40261 to 0.5 mL tube 61 and 10μl of DNA plasmid 40262 to 0.5 mL tube 62

7.

Incubated 0.5 mL tubes at 37 degrees Celsius for fifteen minutes

8.

Added 10μl purple 6x loading dye to each of the 0.5 mL tube and mixed

9.

Gel was loaded as follows:

	A	B	C	D	E	F	G
1	Cane	1	2	3	4	5	6
2	10μl of 2 log ladder	10μl of digested ladder	10μl of digested 40262	15μl of digested 40262	15μl of digested 40261	15μl of digested 40261	10μl of 2 log ladder

Table1

Re-Running Gel Electrophoresis

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-10-10

Tuesday, 10/10/17

1.

Repeat of 10/6/17 procedure from Step 5

2.

Placed unused gel in gel box for 30 min

3.

Pipetted 10μl in 1st and last lane (purple ladder)

4.

Pipetted 15μl in 2nd and 2nd to last lane (3x concentration ol-digest)

5.

Pipetted 30μl (double bond) in outer lanes

6.

Make ETBR solution and place gel in there and on SHAKER (5 min)

7.

Image gel and results: Showed that plasmids were restricted at least once, but 100 bp fragmentts were not seen

8.

Conclusion: Restriction enzymes are to be checked for cut/not.

Re-Run Restrictions on 3x oncnerated samples for 1 hour

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-10-12

Thursday, 10/12/17

1.

Labeled 0.5 mL tubes "61" and "62"

2.

Transferred 5μl of 10x NEBuffer, 1μl of StuI, 1μl of Cac 81, and 33μl H2O

3.

Transferred 10μl of DNA plasmid 40261 to 0.5 mL tube "62"

4.

Incubated tubes at 37 degrees Celsius for 1 hour

5.

Added 10μl 6x purple loading dye to each tube and mixed

6.

Gel was labeled as follows

	A	B	C	D	E	F	G	H
1	Lane	1	2	3	4	5	6	7
2	empty	10μl 2 log buffer	15μl digested 40261	26μl digested 40261	26μl digested 40262	15μl digested 40262	10μl 2 log buffer
3

Table1

Test of DH5Alfa Souls

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-10-12

Thursday, 10/12/17

Gel Extraction and PCR Purification

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-10-13

Friday, 10/13/17

1.

Weighed gel slices containing DNA fragment

a.

61a)0.3620g b)0.3895g

b.

62a) 0.2865g b)0.3877g

2.

Added gel solubilization buffer L3 in 3:1 ratio to gel in a 1.7 mL polypropylene tube

3.

Incubated tubes at 50 degrees Celsius for 15 min and inverted tubes every 3 min

4.

Incubated the tubes for an additional 5 min when gel slice appeared dissolved

5.

Pipetted and dissolved gel piece onto a column at 12000 x g for 1 minute. Discarded flow-through and placed column into wash tube

6.

Added 500μl wash buffer containing ethanol to the column. Centrifuged column at >12000 x g for one minute. DIscarded flow thorugh and placed column into wash tube. Centrifuged column at max speed for 2-3 min. Discarded flow-through.

7.

Placed column into recovery tube. Added 50 mL elution buffer (E1) to the column. INcubated tube for a minute at room temperature. Centrifuged tube > 12000 x g/minute.

8.

Stored, purified DNA at -20 degrees Celsius for immediate use

Purifying Gel Bonds from STUL-XBAL Digest

Project: HD Resolution iGEM Notebook

Authors: HD Resolution iGEM

Date: 2017-10-14

Saturday, 10/14/17

1.

weigh gel slices containing dna fragments

a.

61 > 0.2100 g

b.

62 > 0.2230 g

2.

Add Gel solubilization buffer L3 to excised gels in the tubes in a 3:1 ratop

a.

61 > 0. 63 g = 630μl

b.

62 > 0.669 g = 669μl

3.

Incubate at 50 degrees Celsius for 10 minutes ad invert tube every 3 minutes. After the gel slie appears dissolves, incubate tube for an additional 3 min

4.

Add 1 gel volume of isporopanol to dissolved gel slices. Mix well

a.

61 > 210μl

b.

62 > 223μl

5.

Pipet 850μl of dissolved gel pieces into wash tubes. Centrifuge for

How to Assemble the Parts we currently have

Project: HD Resolution Shared Project

Authors: jason fuller

Date: 2017-10-18

Wednesday, 10/18/17

Assuming Exon 1 Flanks have been properly assembled, the remaing ligations could proceed as follows:

Pair and join every 2 G-Blocks, then PCR amplify (IDT doesn't reccomend PCR amplifying parts greater than 1000 bases)

- Start working backwards (GB15 + GB14, GB13+GB12, etc...)

- Pair the Flanked Exon1 part with GB1

Should work as you are only requring polymerase to amplify 500kb or less

OR

LOW EFFICIENCY - extra work, but probably will work!

Pair 3 sets of 5 G-Blocks using mastermix

Blunt cut a plasmid with a restriction enzyme (peferably one with other restriction sites near by - or where a restriction site will be made where the insert is) CUT in the middle of the GFP!!!!!!!

Blunt assemble

transform

colony pick many colonies (THAT DON'T GLOW)

miniprep separtate colonies

- run gel to look for plasmid of correct size. (on side)

Run Gibson assembly.....

OR

remove phosphates from above plasmid cuts via pcr amplification

add phosphates to gibson assembled g-blocks* (how? / how to separate from other smaller fragments?)

Gibson assemble the 8 parts into 2 separate plasmids (could try 1 but success seesm unlikely):

- prepare a unphosphorlated vector backbone (via reverse amplification PCR is best)

- after mastermix step, phosphoralate the DNA

- Vector insert as shown here: https://youtu.be/-Vh-LbJAJpk?t=19m52s

Need to order?: phosphoralase, dephosphoralase (or primers for the backbone), blunt-end lligase, ligase

Questions to answer ASAP:

Do we have Exon1 properly assmebled?

Do we have the primers needed so we don't have to dephosphoralate?

ToeHold Exchange Questions

Project: HD Resolution Shared Project

Authors: jason fuller

Date: 2017-10-18

Wednesday, 10/18/17

Do we need to assemble better Kozak sequences for our syntheitc mRNA replacement?

WT-Mutated = exact gene

chaperone target footing = fagments of exact gene starting at posion 7 up though position 47

- seems like we shouldn't start later than 30 or sooner than 15

- 22 to

image.png

Kozak Sequences should be evaluated and/or Alternative codon used from positions 7 though 47

Chaperone extension though the start codon could therotically go up though the BbCI cut site at position 209 but is perhaps better to keep short and extend no further than the StuI cut site at positon 11O

image.png

Kozak Sequence is imperfect in WT HTT, syntetic form should be changed to contain the GCCG portion:

image.png

Everything before this sequence doesn't really matter and could use alternative splicing.

Perhaps the sequence can just be trimed before this and the guid strand be the hairpin target overhang?

Else it might be nice for it to be D

Wild Type Huntinton mRNA - GBlock Aligned

What did our PCR actually amplify?

Project: HD Resolution Shared Project

Authors: jason fuller

Date: 2017-10-18

Wednesday, 10/18/17

According to PCR theroy the "short" amplicons swamp out all others during an aplification:

http://www.cybertory.org/exercises/primerDesign/#Background (about 1/2 way down)

Thus, we don't actually need perfect cut sites if we are getting enough sticky ends to start an amplification....

From:

Wild Type Huntinton mRNA - GBlock Aligned

image.png

HD RESOLUTION

hdresolutionigem@gmail.com | Phone: (347) 709-6167

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+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1371">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Wednesday, 9/20/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1375">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1375" data-index="0" style="margin-left:35px;">Innoculated 600 mL LB w/ 1 mL O/N culture </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1376">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1376" data-index="1" style="margin-left:35px;">Shake at 37 degrees Celsius, DDC 600 = 0.4, 3 hr</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1377">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1377" data-index="2" style="margin-left:35px;">Cooled all materials in ice, including solutions (CaCl3), cells, tubes, &amp; tips</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1378">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1378" data-index="3" style="margin-left:35px;">Aliquoted 600 mL of cells into 12 50 mL tubes, 30 mL each </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1379">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1379" data-index="4" style="margin-left:35px;">Spin 1.5 k rcf, 5 min</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1380">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1380" data-index="5" style="margin-left:35px;">Decant sup, resus, pellets in 1 mL CaCl2 (cold) by gentle rocking, left on ice for 30 min </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1381">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1381" data-index="6" style="margin-left:35px;">Spin as above </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1382">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>8.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1382" data-index="7" style="margin-left:35px;">Discard supernatent </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1383">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>9.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1383" data-index="8" style="margin-left:35px;">Added fresh cold 100 mM CaCl3</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1384">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>10.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1384" data-index="9" style="margin-left:35px;">Resuspend as above</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1385">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>11.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1385" data-index="10" style="margin-left:35px;">Added 10.2 mL 50% glycerol to 50 mL falcon tube (chilled) </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1386">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>12.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1386" data-index="11" style="margin-left:35px;">Consolidated all resuspended cells into the tube containing 50% glycerol </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1387">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>13.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1387" data-index="12" style="margin-left:35px;">Did not fully resuspend cells, still looked chunky</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1388">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>14.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1388" data-index="13" style="margin-left:35px;">Froze in 1 mL aliquots of liiquid nitrogen, left in unmarked box below iGEM box in -80 degrees Celsius freezer</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1389">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1389" data-index="14" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1390">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
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+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
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+              </div>
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+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Continuation</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-09-22</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1249">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Friday, 9/22/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1253">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1253" data-index="0" style="margin-left:35px;">Added 175μl of ice cold Alkaline Lysis Solution III through the viscous bacterial lysate. Inverted tube several times and stored tube on ice for 5 min. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1254">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1254" data-index="1" style="margin-left:35px;">Centrifuged lysate at maximum speed for 5 min at 4 degrees Celsius, and transferrred the supernatant to a new tube </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1255">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1255" data-index="2" style="margin-left:35px;">Precipitated the nucleic acids from the supernatant. Added 1 mL of ethanol at room temperature. Mixedsolution by vortexing and allowed mixture to stand for two minutes at room temp. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1256">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1256" data-index="3" style="margin-left:35px;">Collected precipitated nucleic acds by centrifugation at max speed for 5 min at 4 degrees Celsius. Dropped precipitated nucleic aicds. Redid centrifugation at max spped for 5 minutes at 4 degrees Celsius. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1257">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1257" data-index="4" style="margin-left:35px;">Removed supernatant by gentle aspiration, as shown in step 4. Stood tube in an inverted position on a paper towel to allow all the fluid to drain away. Used a kimwipe to remove the drops of fluid adhering to the walls of the tube. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1258">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1258" data-index="5" style="margin-left:35px;">Added 1 mL of 70% ethanol to the pellet and inverted the closed tube several times.</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1259">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1259" data-index="6" style="margin-left:35px;">Recovered DNA by centrifugation at max speed for 2 min at 4 degrees Celsius in a microcentrifuge. </div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
+            </div>
+          </div>
+        </div>
+      </div>
+    </div>
+    <div>
+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
+        <symbol id="protocol_fe1ede5e9a4032ec83f06fc2b26ab3ab" viewbox="0 0 16 16">
+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>protocol</title>
+          <desc>Created with Sketch.</desc>
+          <defs></defs>
+          <path d="M1.5,1.99406028 L1.5,1.99406028 L1.5,14.0059397 C1.5,14.2778635 1.72200248,14.5 1.99406028,14.5 L14.0059397,14.5 C14.2778635,14.5 14.5,14.2779975 14.5,14.0059397 L14.5,1.99406028 C14.5,1.72213649 14.2779975,1.5 14.0059397,1.5 L1.99406028,1.5 C1.72213649,1.5 1.5,1.72200248 1.5,1.99406028 L1.5,1.99406028 Z M1.99406028,0 L14.0059397,0 C15.1072288,0 16,0.894513756 16,1.99406028 L16,14.0059397 C16,15.1072288 15.1054862,16 14.0059397,16 L1.99406028,16 C0.892771196,16 0,15.1054862 0,14.0059397 L0,1.99406028 C0,0.892771196 0.894513756,0 1.99406028,0 Z M4,5 C4.55228475,5 5,4.55228475 5,4 C5,3.44771525 4.55228475,3 4,3 C3.44771525,3 3,3.44771525 3,4 C3,4.55228475 3.44771525,5 4,5 Z M7,5 L12,5 C12.5522847,5 13,4.55228475 13,4 C13,3.44771525 12.5522847,3 12,3 L7,3 C6.44771525,3 6,3.44771525 6,4 C6,4.55228475 6.44771525,5 7,5 Z M7,9 L12,9 C12.5522847,9 13,8.55228475 13,8 C13,7.44771525 12.5522847,7 12,7 L7,7 C6.44771525,7 6,7.44771525 6,8 C6,8.55228475 6.44771525,9 7,9 Z M7,13 L12,13 C12.5522847,13 13,12.5522847 13,12 C13,11.4477153 12.5522847,11 12,11 L7,11 C6.44771525,11 6,11.4477153 6,12 C6,12.5522847 6.44771525,13 7,13 Z M4,9 C4.55228475,9 5,8.55228475 5,8 C5,7.44771525 4.55228475,7 4,7 C3.44771525,7 3,7.44771525 3,8 C3,8.55228475 3.44771525,9 4,9 Z M4,13 C4.55228475,13 5,12.5522847 5,12 C5,11.4477153 4.55228475,11 4,11 C3.44771525,11 3,11.4477153 3,12 C3,12.5522847 3.44771525,13 4,13 Z" id="protocol_fe1ede5e9a4032ec83f06fc2b26ab3ab_Combined-Shape"></path>
+        </symbol>
+      </svg>
+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Tranformation</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-09-25</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1609">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Monday, 9/25/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1613">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1613" data-index="0" style="margin-left:35px;">Thaw DHS-Alpa Cells on ice</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1614">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1614" data-index="1" style="margin-left:35px;">Add 2 mL of DNA to cells (from previously isolated adgene plasmids)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1615">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1615" data-index="2" style="margin-left:35px;">Stand for 34 min on ice </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1616">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1616" data-index="3" style="margin-left:35px;">Cells are incubated for 60 sec at 42 degrees Celsius</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1617">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1617" data-index="4" style="margin-left:35px;">Put cells on ice for 5 minut s</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1618">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1618" data-index="5" style="margin-left:35px;">Add 900μl SOC media </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1619">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1619" data-index="6" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
+            </div>
+          </div>
+        </div>
+      </div>
+    </div>
+    <div>
+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
+        <symbol id="protocol_fe1ede5e9a4032ec83f06fc2b26ab3ab" viewbox="0 0 16 16">
+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>protocol</title>
+          <desc>Created with Sketch.</desc>
+          <defs></defs>
+          <path d="M1.5,1.99406028 L1.5,1.99406028 L1.5,14.0059397 C1.5,14.2778635 1.72200248,14.5 1.99406028,14.5 L14.0059397,14.5 C14.2778635,14.5 14.5,14.2779975 14.5,14.0059397 L14.5,1.99406028 C14.5,1.72213649 14.2779975,1.5 14.0059397,1.5 L1.99406028,1.5 C1.72213649,1.5 1.5,1.72200248 1.5,1.99406028 L1.5,1.99406028 Z M1.99406028,0 L14.0059397,0 C15.1072288,0 16,0.894513756 16,1.99406028 L16,14.0059397 C16,15.1072288 15.1054862,16 14.0059397,16 L1.99406028,16 C0.892771196,16 0,15.1054862 0,14.0059397 L0,1.99406028 C0,0.892771196 0.894513756,0 1.99406028,0 Z M4,5 C4.55228475,5 5,4.55228475 5,4 C5,3.44771525 4.55228475,3 4,3 C3.44771525,3 3,3.44771525 3,4 C3,4.55228475 3.44771525,5 4,5 Z M7,5 L12,5 C12.5522847,5 13,4.55228475 13,4 C13,3.44771525 12.5522847,3 12,3 L7,3 C6.44771525,3 6,3.44771525 6,4 C6,4.55228475 6.44771525,5 7,5 Z M7,9 L12,9 C12.5522847,9 13,8.55228475 13,8 C13,7.44771525 12.5522847,7 12,7 L7,7 C6.44771525,7 6,7.44771525 6,8 C6,8.55228475 6.44771525,9 7,9 Z M7,13 L12,13 C12.5522847,13 13,12.5522847 13,12 C13,11.4477153 12.5522847,11 12,11 L7,11 C6.44771525,11 6,11.4477153 6,12 C6,12.5522847 6.44771525,13 7,13 Z M4,9 C4.55228475,9 5,8.55228475 5,8 C5,7.44771525 4.55228475,7 4,7 C3.44771525,7 3,7.44771525 3,8 C3,8.55228475 3.44771525,9 4,9 Z M4,13 C4.55228475,13 5,12.5522847 5,12 C5,11.4477153 4.55228475,11 4,11 C3.44771525,11 3,11.4477153 3,12 C3,12.5522847 3.44771525,13 4,13 Z" id="protocol_fe1ede5e9a4032ec83f06fc2b26ab3ab_Combined-Shape"></path>
+        </symbol>
+      </svg>
+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Preparation of LB-Agar Plates</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-09-26</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1625">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Tuesday, 9/26/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1629">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1629" data-index="0" style="margin-left:0;">Preparation of LB-Agar Plates </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1630">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1630" data-index="1" style="margin-left:0;">- Adapted from CSH Protocols </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1631">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1631" data-index="2" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1632">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1632" data-index="3" style="margin-left:0;">LB Agar </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1633">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1633" data-index="4" style="margin-left:0;">- 3.75 gramf of Agar </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1634">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1634" data-index="5" style="margin-left:0;">- 250 mL of 1x LB Medium </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1635">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1635" data-index="6" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1636">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1636" data-index="7" style="margin-left:35px;">Mix </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1637">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1637" data-index="8" style="margin-left:35px;">Autoclave on Liquid Cycle for 30 minutes </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1638">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1638" data-index="9" style="margin-left:70px;">Do not overfill bottles</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1639">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:105px;"><span>I.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1639" data-index="10" style="margin-left:105px;">250 mL of LB was autoclaved in a 500 mL bottle to prevent it from boiling over. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1640">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1640" data-index="11" style="margin-left:35px;">Add 2.5 mL of Kanamycin Stock (25 mg/mL) to a final concentration of 25ug/mL, once LB-Agar has coded to about 50 degrees Celsius. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1641">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1641" data-index="12" style="margin-left:35px;">Heat on a hot plate to re-melt sugar </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1642">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1642" data-index="13" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
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+          </div>
+        </div>
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+    </div>
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+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Running Electrophoresis Gel of ADDGENE Plasmid Digests</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-09-26</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1546">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Tuesday, 9/26/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1550">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1550" data-index="0" style="margin-left:35px;">Cut up unused gel and add to remaining unused 2% agarose state. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1551">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1551" data-index="1" style="margin-left:35px;">Warm until gel molds / 2.5 for 5 min </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1552">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1552" data-index="2" style="margin-left:35px;">Pour into casting pan </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1553">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1553" data-index="3" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
+            </div>
+          </div>
+        </div>
+      </div>
+    </div>
+    <div>
+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"></svg>
+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Interlab &amp; Second Competent Cell Test</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-09-26</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1311">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Tuesday, 9/26/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1315">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1315" data-index="0" style="margin-left:35px;">Clean working area</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1316">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1316" data-index="1" style="margin-left:35px;">Thaw competent cells on ice </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1317">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1317" data-index="2" style="margin-left:35px;">Label 8 microcentrifuge tubes : 10ug, 50ug, 100ug, 1, 4, 5, 6, Neg.</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1318">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1318" data-index="3" style="margin-left:35px;">Spin down DNA from competent cell test kit and interlab DNA </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1319">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1319" data-index="4" style="margin-left:35px;">Pipet 1μl of DN from competent cell test kit into tubes labeled 1, 4, 5, 6, neg </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1320">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1320" data-index="5" style="margin-left:35px;">Pipet 85μl of competent cells into all tubes, flick gently to mix </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1321">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1321" data-index="6" style="margin-left:35px;">Incubate on ice for 30 minutes </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1322">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>8.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1322" data-index="7" style="margin-left:35px;">Heat shock cells by placing tubes into a hot water bath at 42 degrees Celsius for 45 seconds </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1323">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>9.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1323" data-index="8" style="margin-left:35px;">Transfer back and  incubate on ice for 5 min </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1324">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>10.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1324" data-index="9" style="margin-left:35px;">Add 45μl of SOC Media into eah cand incubate at 37 degrees for 1 hour shaking at 200-300 rpm</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1325">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>11.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1325" data-index="10" style="margin-left:35px;">Warm up agar plates, label </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1326">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>12.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1326" data-index="11" style="margin-left:35px;">Pipet 100μl from each tube onto appropriate plate, spread mixture evenly across plates via sterile glass beads. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1327">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>13.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1327" data-index="12" style="margin-left:35px;">Close with parafilm</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1328">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>14.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1328" data-index="13" style="margin-left:35px;">Incubate at 37 degrees Celsius overnight w/ plates positioned w/ agar side at top and lid at bottom </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1329">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1329" data-index="14" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
+            </div>
+          </div>
+        </div>
+      </div>
+    </div>
+    <div>
+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"></svg>
+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Interlab</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-09-27</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1249">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Wednesday, 9/27/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1253">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1253" data-index="0" style="margin-left:35px;">Clean working area </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1254">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1254" data-index="1" style="margin-left:35px;">Pipette 1μl of 1, 4, 5, 6, neg - and control each with their respective cell tube </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1255">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1255" data-index="2" style="margin-left:35px;">INcubate for 30 min (until 7:02) </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1256">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1256" data-index="3" style="margin-left:35px;">Pipette 100μl of LUDOX into wells A1, B1, C1. D1 each </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1257">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1257" data-index="4" style="margin-left:35px;">Pipette 100μl of H2O each into wells A2, B2, C2, D2 </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1258">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1258" data-index="5" style="margin-left:35px;">Spin down fluorescein tube </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1259">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1259" data-index="6" style="margin-left:35px;">Resuspend 100μM florescein solution in 1 mL 1xPBS to make 2x solution </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1260">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>8.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1260" data-index="7" style="margin-left:35px;">Dilute to 1x. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1261">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>9.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1261" data-index="8" style="margin-left:35px;">Perform serial dilution with 10 columns of 1x PBS (leave an additional column as regular PBS) </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1262">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
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+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Restriction Digest on Previously Isolated DNA Plasmid</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-10-06</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1525">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Friday, 10/6/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1529">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1529" data-index="0" style="margin-left:35px;">Placed previously isolated DNA plasmid on ie (61 and 62)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1530">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1530" data-index="1" style="margin-left:35px;">Measured the concentration of the DNA Plasmids with the nanodrop (2μl) </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1531">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1531" data-index="2" style="margin-left:70px;">DNA plasmid 40261 : 356.2 ng/μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1532">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>b.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1532" data-index="3" style="margin-left:70px;">DNA plasmid 40262 : 300.5 ng/μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1533">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1533" data-index="4" style="margin-left:35px;">Diluted DNA plasmid 1 : 3 to approximate 100 ng/μl and measured with nanodrop </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1534">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1534" data-index="5" style="margin-left:70px;">DNA plasmid 40261 : 117.9 ng/μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1535">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>b.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1535" data-index="6" style="margin-left:70px;">DNA plasmid 40262 : 106.1 ng/μl  </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1536">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1536" data-index="7" style="margin-left:35px;">Labeled fresh 0.5 mL tubes 61 and 62 </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1537">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1537" data-index="8" style="margin-left:35px;">For each tube, transferred 5 ul of 10x NEBuffer, 1 ul of Stul, 1 mL of cac 81, and 33 ul of dH2O</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1538">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1538" data-index="9" style="margin-left:35px;">Transferred 10μl DNA Plasmid 40261 to 0.5 mL tube 61 and 10μl of DNA plasmid 40262 to 0.5 mL tube 62 </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1539">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1539" data-index="10" style="margin-left:35px;">Incubated 0.5 mL tubes at 37 degrees Celsius for fifteen minutes </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1540">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>8.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1540" data-index="11" style="margin-left:35px;">Added 10μl purple 6x loading dye to each of the 0.5 mL tube and mixed </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1541">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>9.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1541" data-index="12" style="margin-left:35px;">Gel was loaded as follows: </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-captionedTable" data-scroll-id="note-part1542">
+                          <div>
+                            <div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1542-item" data-formattable="true" data-index="13" data-subindex="item"></div>
+                            <div class="mediocre-captionEditable multi-editable mediocre-captionEditable--table">
+                              <div class="mediocre-captionEditable-indent">
+                                <div class="mediocre-captionEditable-grid">
+                                  <div class="mediocre-captionEditable-row">
+                                    <div class="mediocre-captionEditable-cell"></div>
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;">
+                                      <div class="note-item-wrapper">
+                                        <div class="mediocre-tableEditable printable-container-scaleForPrint" data-formattable="true" data-index="13" data-subindex="cell" style="margin-left:0;margin-right:0;">
+                                          <div></div>
+                                          <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                                          <div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1542-table" data-formattable="true" data-index="13" data-subindex="table"></div>
+                                          <div class="mediocre-tableEditable-scrollArea">
+                                            <div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;">
+                                              <div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:765px;"></div>
+                                              <table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:745px;">
+                                                <colgroup>
+                                                  <col style="width:45px;">
+                                                  </col>
+                                                  <col data-id="0" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="1" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="2" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="3" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="4" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="5" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="6" style="width:100px;">
+                                                  </col>
+                                                </colgroup>
+                                                <tbody>
+                                                  <tr>
+                                                    <td class="mediocre-tableEditable-axisCell u-no-mouse-target"></td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div>
+                                                    </td>
+                                                  </tr>
+                                                  <tr>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div>
+                                                    </td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-0-0" data-namespace="table-ns1767"><span>Cane </span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1542-0-1" data-namespace="table-ns1767"><span>1</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1542-0-2" data-namespace="table-ns1767"><span>2</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1542-0-3" data-namespace="table-ns1767"><span>3</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1542-0-4" data-namespace="table-ns1767"><span>4</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1542-0-5" data-namespace="table-ns1767"><span>5</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1542-0-6" data-namespace="table-ns1767"><span>6</span></td>
+                                                  </tr>
+                                                  <tr>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div>
+                                                    </td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-0" data-namespace="table-ns1767"><span>10μl of 2 log ladder</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-1" data-namespace="table-ns1767"><span>10μl of digested ladder </span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-2" data-namespace="table-ns1767"><span>10μl of digested 40262 </span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-3" data-namespace="table-ns1767"><span>15μl of digested 40262 </span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-4" data-namespace="table-ns1767"><span>15μl of digested 40261</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-5" data-namespace="table-ns1767"><span>15μl of digested 40261</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1542-1-6" data-namespace="table-ns1767"><span>10μl of 2 log ladder </span></td>
+                                                  </tr>
+                                                </tbody>
+                                              </table>
+                                            </div>
+                                          </div>
+                                          <div class="mediocre-tableEditable-titleBarWrapper" style="left:0;">
+                                            <div class="mediocre-tableEditable-titleBar">
+                                              <div class="mediocre-tableEditable-tableName">
+                                                <div class="u-flex-grow u-ellipsis-text">Table1</div>
+                                                <div class="u-flex-none"></div>
+                                              </div>
+                                              <div class="u-flex-none u-tiny-spacing-left table-floater"></div>
+                                            </div>
+                                          </div>
+                                        </div>
+                                      </div>
+                                    </div>
+                                    <div class="mediocre-captionEditable-cell"></div>
+                                  </div>
+                                  <div class="mediocre-captionEditable-row">
+                                    <div class="mediocre-captionEditable-cell"></div>
+                                    <div class="mediocre-captionEditable-cell">
+                                      <div class="mediocre-captionEditable-caption--bottom mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1542" data-index="13" style="max-width:765px;margin-left:0;"></div>
+                                    </div>
+                                    <div class="mediocre-captionEditable-cell"></div>
+                                  </div>
+                                </div>
+                              </div>
+                            </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1543">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1543" data-index="14" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1544">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1544" data-index="15" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1545">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1545" data-index="16" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
+            </div>
+          </div>
+        </div>
+      </div>
+    </div>
+    <div>
+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
+        <symbol id="protocol_fe1ede5e9a4032ec83f06fc2b26ab3ab" viewbox="0 0 16 16">
+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>protocol</title>
+          <desc>Created with Sketch.</desc>
+          <defs></defs>
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+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Re-Running Gel Electrophoresis</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-10-10</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1354">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Tuesday, 10/10/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1358">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1358" data-index="0" style="margin-left:35px;">Repeat of 10/6/17 procedure from Step 5 </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1359">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1359" data-index="1" style="margin-left:35px;">Placed unused gel in gel box for 30 min </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1360">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1360" data-index="2" style="margin-left:35px;">Pipetted 10μl in 1st and last lane (purple ladder) </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1361">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1361" data-index="3" style="margin-left:35px;">Pipetted 15μl in 2nd and 2nd to last lane (3x concentration ol-digest)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1362">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1362" data-index="4" style="margin-left:35px;">Pipetted 30μl (double bond) in outer lanes </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1363">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1363" data-index="5" style="margin-left:35px;">Make ETBR solution and place gel in there and on SHAKER (5 min) </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1364">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1364" data-index="6" style="margin-left:35px;">Image gel and results: Showed that plasmids were restricted at least once, but 100 bp fragmentts were not seen </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1365">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>8.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1365" data-index="7" style="margin-left:35px;">Conclusion: Restriction enzymes are to be checked for cut/not. </div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
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+      </div>
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+          <title>protocol</title>
+          <desc>Created with Sketch.</desc>
+          <defs></defs>
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+      </svg>
+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Re-Run Restrictions on 3x oncnerated samples for 1 hour</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-10-12</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1643">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Thursday, 10/12/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1647">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1647" data-index="0" style="margin-left:35px;">Labeled 0.5 mL tubes "61" and "62" </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1648">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1648" data-index="1" style="margin-left:35px;">Transferred 5μl of 10x NEBuffer, 1μl of StuI, 1μl of Cac 81, and 33μl H2O </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1649">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1649" data-index="2" style="margin-left:35px;">Transferred 10μl of DNA plasmid 40261 to 0.5 mL tube "62"</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1650">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1650" data-index="3" style="margin-left:35px;">Incubated tubes at 37 degrees Celsius for 1 hour </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1651">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1651" data-index="4" style="margin-left:35px;">Added 10μl 6x purple loading dye to each tube and mixed </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1652">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1652" data-index="5" style="margin-left:35px;">Gel was labeled as follows </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1653">
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+                                              <table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:845px;">
+                                                <colgroup>
+                                                  <col style="width:45px;">
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+                                                  </col>
+                                                  <col data-id="2" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="3" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="4" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="5" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="6" style="width:100px;">
+                                                  </col>
+                                                  <col data-id="7" style="width:100px;">
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+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div>
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+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div>
+                                                    </td>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div>
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+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div>
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+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div>
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+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div>
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+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div>
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+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div>
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+                                                  </tr>
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+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div>
+                                                    </td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-0-0" data-namespace="table-ns1787"><span>Lane</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-1" data-namespace="table-ns1787"><span>1</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-2" data-namespace="table-ns1787"><span>2</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-3" data-namespace="table-ns1787"><span>3</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-4" data-namespace="table-ns1787"><span>4</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-5" data-namespace="table-ns1787"><span>5</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-6" data-namespace="table-ns1787"><span>6</span></td>
+                                                    <td class="cell is-value is-alignRight cell-contents" data-cid="table-note-part1654-0-7" data-namespace="table-ns1787"><span>7</span></td>
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+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div>
+                                                    </td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-0" data-namespace="table-ns1787"><span>empty</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-1" data-namespace="table-ns1787"><span>10μl 2 log buffer</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-2" data-namespace="table-ns1787"><span>15μl digested 40261 </span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-3" data-namespace="table-ns1787"><span>26μl digested 40261 </span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-4" data-namespace="table-ns1787"><span>26μl digested 40262</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-5" data-namespace="table-ns1787"><span>15μl digested 40262</span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-6" data-namespace="table-ns1787"><span>10μl 2 log buffer </span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-1-7" data-namespace="table-ns1787"><span></span></td>
+                                                  </tr>
+                                                  <tr>
+                                                    <td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row">
+                                                      <div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div>
+                                                    </td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-0" data-namespace="table-ns1787"><span></span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-1" data-namespace="table-ns1787"><span></span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-2" data-namespace="table-ns1787"><span></span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-3" data-namespace="table-ns1787"><span></span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-4" data-namespace="table-ns1787"><span></span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-5" data-namespace="table-ns1787"><span></span></td>
+                                                    <td class="cell is-text is-alignLeft cell-contents" data-cid="table-note-part1654-2-6" data-namespace="table-ns1787"><span></span></td>
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+                                                  </tr>
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+          <title>protocol</title>
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+                      <h1 class="mediocre-titleEditor">
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+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-10-12</div>
+                      </div>
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+                          <span class="daySeparator-date">Thursday, 10/12/17</span>
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+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Gel Extraction and PCR Purification</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-10-13</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1414">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Friday, 10/13/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1418">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1418" data-index="0" style="margin-left:35px;">Weighed gel slices containing DNA fragment </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1419">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1419" data-index="1" style="margin-left:70px;">61a)0.3620g    b)0.3895g </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1420">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>b.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1420" data-index="2" style="margin-left:70px;">62a) 0.2865g  b)0.3877g</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1421">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1421" data-index="3" style="margin-left:35px;">Added gel solubilization buffer L3 in 3:1 ratio to gel in a 1.7 mL polypropylene tube </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1422">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1422" data-index="4" style="margin-left:35px;">Incubated tubes at 50 degrees Celsius for 15 min and inverted tubes every 3 min </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1423">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1423" data-index="5" style="margin-left:35px;">Incubated the tubes for an additional 5 min when gel slice appeared dissolved </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1424">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1424" data-index="6" style="margin-left:35px;">Pipetted and dissolved gel piece onto a column at 12000 x g for 1 minute. Discarded flow-through and placed column into wash tube </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1425">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1425" data-index="7" style="margin-left:35px;">Added 500μl wash buffer containing ethanol to the column. Centrifuged column at &gt;12000 x g for one minute. DIscarded flow thorugh and placed column into wash tube. Centrifuged column at max speed for 2-3 min. Discarded flow-through. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1426">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1426" data-index="8" style="margin-left:35px;">Placed column into recovery tube. Added 50 mL elution buffer (E1) to the column. INcubated tube for a minute at room temperature. Centrifuged tube &gt; 12000 x g/minute. </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1427">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>8.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1427" data-index="9" style="margin-left:35px;">Stored, purified DNA at -20 degrees Celsius for immediate use</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1428">
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+                        </div>
+                      </div>
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+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>protocol</title>
+          <desc>Created with Sketch.</desc>
+          <defs></defs>
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+        <div class="printable-container hide-print-links">
+          <div>
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+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Purifying Gel Bonds from STUL-XBAL Digest</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution iGEM Notebook</div>
+                        <div><b>Authors:</b> HD Resolution iGEM</div>
+                        <div><b>Date:</b> 2017-10-14</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1583">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Saturday, 10/14/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1587">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1587" data-index="0" style="margin-left:35px;">weigh gel slices containing dna fragments </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1588">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1588" data-index="1" style="margin-left:70px;">61 &gt; 0.2100 g</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1589">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>b.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1589" data-index="2" style="margin-left:70px;">62 &gt; 0.2230 g </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1590">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1590" data-index="3" style="margin-left:35px;">Add Gel solubilization buffer L3 to excised gels in the tubes in a 3:1 ratop </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1591">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1591" data-index="4" style="margin-left:70px;">61 &gt; 0. 63 g = 630μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1592">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>b.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1592" data-index="5" style="margin-left:70px;">62 &gt; 0.669 g = 669μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1593">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1593" data-index="6" style="margin-left:35px;">Incubate at 50 degrees Celsius for 10 minutes ad invert tube every 3 minutes. After the gel slie appears dissolves, incubate tube for an additional 3 min</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1594">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1594" data-index="7" style="margin-left:35px;">Add 1 gel volume of isporopanol to dissolved gel slices. Mix well</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1595">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1595" data-index="8" style="margin-left:70px;">61 &gt; 210μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1596">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:70px;"><span>b.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1596" data-index="9" style="margin-left:70px;">62 &gt; 223μl</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1597">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1597" data-index="10" style="margin-left:35px;">Pipet 850μl of dissolved gel pieces into wash tubes. Centrifuge for </div>
+                          </div>
+                        </div>
+                      </div>
+                    </div>
+                    <div class="dnd-drop-container-border"></div>
+                  </div>
+                </div>
+                <div class="noteEditor-nueModuleContainer"></div>
+              </div>
+            </div>
+          </div>
+        </div>
+      </div>
+    </div>
+    <div>
+      <svg style="position: absolute; width: 0; height: 0" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
+        <symbol id="sequence_b33ff1e4a9d7f262694f1b0d0b74a27b" viewbox="0 0 16 16">
+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>sequence</title>
+          <desc>Created with Sketch.</desc>
+          <defs></defs>
+          <path d="M0,8 C0,12.418278 3.581722,16 8,16 C12.418278,16 16,12.418278 16,8 C16,3.92038235 12.9463116,0.553991843 9,0.0618937549 L9,1.82956975 C11.9764258,2.30820976 14.25,4.88859403 14.25,8 C14.25,11.4517797 11.4517797,14.25 8,14.25 C4.54822031,14.25 1.75,11.4517797 1.75,8 C1.75,4.88859403 4.02357418,2.30820976 7,1.82956975 L7,0.0618937549 C3.05368842,0.553991843 0,3.92038235 0,8 Z" id="sequence_b33ff1e4a9d7f262694f1b0d0b74a27b_Combined-Shape"></path>
+          <rect height="8" id="sequence_b33ff1e4a9d7f262694f1b0d0b74a27b_Rectangle-5" width="8" x="-6" y="-6"></rect>
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+      </svg>
+      <div>
+        <div class="printable-container hide-print-links">
+          <div>
+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">How to Assemble the Parts we currently have</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution Shared Project</div>
+                        <div><b>Authors:</b> jason fuller</div>
+                        <div><b>Date:</b> 2017-10-18</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1385">
+                          <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
+                          <span class="daySeparator-date">Wednesday, 10/18/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1389">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1389" data-index="0" style="margin-left:0;">Assuming Exon 1 Flanks have been properly assembled, the remaing ligations could proceed as follows:</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1390">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1390" data-index="1" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1391">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1391" data-index="2" style="margin-left:0;">Pair and join every 2 G-Blocks, then PCR amplify (IDT doesn't reccomend PCR amplifying parts greater than 1000 bases)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1392">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1392" data-index="3" style="margin-left:35px;">- Start working backwards (GB15 + GB14, GB13+GB12, etc...)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1393">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1393" data-index="4" style="margin-left:35px;">- Pair the Flanked Exon1 part with GB1</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1394">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1394" data-index="5" style="margin-left:35px;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1395">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1395" data-index="6" style="margin-left:35px;">Should work as you are only requring polymerase to amplify 500kb or less</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1396">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1396" data-index="7" style="margin-left:35px;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1397">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1397" data-index="8" style="margin-left:35px;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1398">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1398" data-index="9" style="margin-left:35px;">OR</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1399">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1399" data-index="10" style="margin-left:0;">LOW EFFICIENCY - extra work, but probably will work!</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1400">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1400" data-index="11" style="margin-left:0;">Pair 3 sets of 5 G-Blocks using mastermix</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1401">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1401" data-index="12" style="margin-left:0;">Blunt cut a plasmid with a restriction enzyme (peferably one with other restriction sites near by - or where a restriction site will be made where the insert is)  CUT in the middle of the GFP!!!!!!!</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1402">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1402" data-index="13" style="margin-left:0;">Blunt assemble</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1403">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1403" data-index="14" style="margin-left:0;">transform </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1404">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1404" data-index="15" style="margin-left:0;">colony pick many colonies (THAT DON'T GLOW)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1405">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1405" data-index="16" style="margin-left:0;">miniprep separtate colonies</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1406">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1406" data-index="17" style="margin-left:0;"> - run gel to look for plasmid of correct size. (on side)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1407">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1407" data-index="18" style="margin-left:0;">Run Gibson assembly.....</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1408">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1408" data-index="19" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1409">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1409" data-index="20" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1410">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1410" data-index="21" style="margin-left:0;">OR</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1411">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1411" data-index="22" style="margin-left:0;">remove phosphates from above plasmid cuts via pcr amplification</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1412">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1412" data-index="23" style="margin-left:0;">add phosphates to gibson assembled g-blocks*   (how? / how to separate from other smaller fragments?)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1413">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1413" data-index="24" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1414">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1414" data-index="25" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1415">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1415" data-index="26" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1416">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1416" data-index="27" style="margin-left:0;">Gibson assemble the 8 parts into 2 separate plasmids (could try 1 but success seesm unlikely):</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1417">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1417" data-index="28" style="margin-left:35px;">- prepare a unphosphorlated vector backbone (via reverse amplification PCR is best)</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1418">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1418" data-index="29" style="margin-left:35px;">- after mastermix step, phosphoralate the DNA</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1419">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1419" data-index="30" style="margin-left:35px;">-  Vector insert as shown here: https://youtu.be/-Vh-LbJAJpk?t=19m52s</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1420">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1420" data-index="31" style="margin-left:35px;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1421">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1421" data-index="32" style="margin-left:35px;">Need to order?:  phosphoralase, dephosphoralase (or primers for the backbone), blunt-end lligase, ligase</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1422">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1422" data-index="33" style="margin-left:35px;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1423">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1423" data-index="34" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1424">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1424" data-index="35" style="margin-left:0;">Questions to answer ASAP:</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1425">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1425" data-index="36" style="margin-left:0;">Do we have Exon1 properly assmebled?</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1426">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1426" data-index="37" style="margin-left:0;">Do we have the primers needed so we don't have to dephosphoralate?</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1427">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
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+                          </div>
+                        </div>
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+                          </div>
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+                          </div>
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+                          </div>
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+                          </div>
+                        </div>
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+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>sequence</title>
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+        <div class="printable-container hide-print-links">
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+            <div class="printable-page-container">
+              <div class="noteEditor mediocre" tabindex="-1">
+                <div></div>
+                <div class="noteEditor-container">
+                  <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">ToeHold Exchange Questions</div>
+                        <span></span>
+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution Shared Project</div>
+                        <div><b>Authors:</b> jason fuller</div>
+                        <div><b>Date:</b> 2017-10-18</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
+                        <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
+                        <div class="daySeparator" data-scroll-id="entryDay1324">
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+                          <span class="daySeparator-date">Wednesday, 10/18/17</span>
+                          <div class="daySeparator-line"></div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1328">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1328" data-index="0" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Do we need to assemble better </span></span><b><span style="color:#344043"><span style="color:#333333"><span style="background-color:#FFFFFF">Kozak sequences </span></span></span></b><span style="color:#333333"><span style="background-color:#FFFFFF">for our syntheitc mRNA replacement?</span></span></div>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1329">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1329" data-index="1" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1330">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1330" data-index="2" style="margin-left:0;">WT-Mutated = exact gene</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1331">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1331" data-index="3" style="margin-left:0;">chaperone target footing = fagments of exact gene starting at posion 7 up though position 47  </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1332">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1332" data-index="4" style="margin-left:35px;">- seems like we shouldn't start later than 30 or sooner than 15</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1333">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:35px;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1333" data-index="5" style="margin-left:35px;">- 22 to </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1334">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1334" data-index="6" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1335">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1335" data-index="7" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item" data-scroll-id="note-part1336">
+                          <div>
+                            <div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1336-item" data-formattable="true" data-index="8" data-subindex="item"></div>
+                            <div class="mediocre-captionEditable multi-editable">
+                              <div class="mediocre-captionEditable-indent" style="padding-left:0;">
+                                <div class="mediocre-captionEditable-grid">
+                                  <div class="mediocre-captionEditable-row">
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-component">
+                                      <div class="note-item-wrapper">
+                                        <div class="dnd-drop-container">
+                                          <div class="noteLinkedFilePreview mediocre-itemSelect note-item">
+                                            <div>
+                                              <h5 class="u-no-spacing-top u-no-spacing-bottom">
+                                                <div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div>
+                                                <span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span>
+                                                <div class="note-itemName">image.png</div>
+                                              </h5>
+                                              <div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="GQpCiNV0IHdt2XsRV9GOqBGXPD7AqVIjj3joloSm-image.png"/></div>
+                                            </div>
+                                          </div>
+                                          <div class="dnd-drop-container-border"></div>
+                                        </div>
+                                      </div>
+                                    </div>
+                                    <div class="mediocre-captionEditable-cell" style="width:0;"></div>
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1336" data-index="8"></div>
+                                  </div>
+                                </div>
+                              </div>
+                            </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1338">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1338" data-index="9" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1339">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1339" data-index="10" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1340">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1340" data-index="11" style="margin-left:0;">Kozak Sequences should be evaluated and/or Alternative codon used from positions 7 though 47</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1341">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1341" data-index="12" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1342">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1342" data-index="13" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1343">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1343" data-index="14" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1344">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1344" data-index="15" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1345">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1345" data-index="16" style="margin-left:0;">Chaperone extension though the start codon could therotically go up though the BbCI cut site at position 209  but is perhaps better to keep short and extend no further than the StuI cut site at positon 11O</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1346">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1346" data-index="17" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item" data-scroll-id="note-part1347">
+                          <div>
+                            <div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1347-item" data-formattable="true" data-index="18" data-subindex="item"></div>
+                            <div class="mediocre-captionEditable multi-editable">
+                              <div class="mediocre-captionEditable-indent" style="padding-left:0;">
+                                <div class="mediocre-captionEditable-grid">
+                                  <div class="mediocre-captionEditable-row">
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-component">
+                                      <div class="note-item-wrapper">
+                                        <div class="dnd-drop-container">
+                                          <div class="noteLinkedFilePreview mediocre-itemSelect note-item">
+                                            <div>
+                                              <h5 class="u-no-spacing-top u-no-spacing-bottom">
+                                                <div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div>
+                                                <span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span>
+                                                <div class="note-itemName">image.png</div>
+                                              </h5>
+                                              <div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="nrSJWAOwQ38v0Guo72Siq4OcBCCuPSLHLU6Nme8N-image.png"/></div>
+                                            </div>
+                                          </div>
+                                          <div class="dnd-drop-container-border"></div>
+                                        </div>
+                                      </div>
+                                    </div>
+                                    <div class="mediocre-captionEditable-cell" style="width:0;"></div>
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1347" data-index="18"></div>
+                                  </div>
+                                </div>
+                              </div>
+                            </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1349">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1349" data-index="19" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1350">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1350" data-index="20" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1351">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1351" data-index="21" style="margin-left:0;">Kozak Sequence is imperfect in WT HTT, syntetic form should be changed to contain the GCCG portion:</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1352">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1352" data-index="22" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item" data-scroll-id="note-part1353">
+                          <div>
+                            <div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1353-item" data-formattable="true" data-index="23" data-subindex="item"></div>
+                            <div class="mediocre-captionEditable multi-editable">
+                              <div class="mediocre-captionEditable-indent" style="padding-left:0;">
+                                <div class="mediocre-captionEditable-grid">
+                                  <div class="mediocre-captionEditable-row">
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-component">
+                                      <div class="note-item-wrapper">
+                                        <div class="dnd-drop-container">
+                                          <div class="noteLinkedFilePreview mediocre-itemSelect note-item">
+                                            <div>
+                                              <h5 class="u-no-spacing-top u-no-spacing-bottom">
+                                                <div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div>
+                                                <span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span>
+                                                <div class="note-itemName">image.png</div>
+                                              </h5>
+                                              <div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="cVqxoUvwoE4BSLIvp1cuE1j8oH320cnPwJym0nil-image.png"/></div>
+                                            </div>
+                                          </div>
+                                          <div class="dnd-drop-container-border"></div>
+                                        </div>
+                                      </div>
+                                    </div>
+                                    <div class="mediocre-captionEditable-cell" style="width:0;"></div>
+                                    <div class="mediocre-captionEditable-cell mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1353" data-index="23"></div>
+                                  </div>
+                                </div>
+                              </div>
+                            </div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1355">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1355" data-index="24" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1356">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1356" data-index="25" style="margin-left:0;">Everything before this sequence doesn't really matter and could use alternative splicing.</div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1357">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1357" data-index="26" style="margin-left:0;"></div>
+                          </div>
+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1358">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1358" data-index="27" style="margin-left:0;">Perhaps the sequence can just be trimed before this and the guid strand be the hairpin target overhang?</div>
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+                        <div class="mediocre-item is-text" data-scroll-id="note-part1359">
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+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1359" data-index="28" style="margin-left:0;"></div>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1360">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1360" data-index="29" style="margin-left:0;">Else it might be nice for it to be D</div>
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+          <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
+          <title>sequence</title>
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+                    <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
+                      <h1 class="mediocre-titleEditor">
+                        <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">What did our PCR actually amplify?</div>
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+                      </h1>
+                      <div>
+                        <div><b>Project:</b> HD Resolution Shared Project</div>
+                        <div><b>Authors:</b> jason fuller</div>
+                        <div><b>Date:</b> 2017-10-18</div>
+                      </div>
+                      <div class="editor" data-editor="notes">
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+                          <span class="daySeparator-date">Wednesday, 10/18/17</span>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1483">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1483" data-index="0" style="margin-left:0;">According to PCR theroy the "short" amplicons swamp out all others during an aplification:</div>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1484">
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+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1484" data-index="1" style="margin-left:0;"></div>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1485">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1485" data-index="2" style="margin-left:0;">http://www.cybertory.org/exercises/primerDesign/#Background (about 1/2 way down)</div>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1486">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1486" data-index="3" style="margin-left:0;"></div>
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+                        </div>
+                        <div class="mediocre-item is-text" data-scroll-id="note-part1487">
+                          <div class="mediocre-noteEditable multi-editable is-paragraph">
+                            <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
+                            <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1487" data-index="4" style="margin-left:0;">Thus, we don't actually need perfect cut sites if we are getting enough sticky ends to start an amplification....</div>
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Difference between revisions of "Team:EpiphanyNYC/Notebook"

Revision as of 04:50, 31 October 2017

Notebook

Testing Competency of Cells

Untitled

Continuation

Tranformation

Preparation of LB-Agar Plates

Running Electrophoresis Gel of ADDGENE Plasmid Digests

Interlab & Second Competent Cell Test

Interlab

Restriction Digest on Previously Isolated DNA Plasmid

Re-Running Gel Electrophoresis

Re-Run Restrictions on 3x oncnerated samples for 1 hour

Test of DH5Alfa Souls

Gel Extraction and PCR Purification

Purifying Gel Bonds from STUL-XBAL Digest

How to Assemble the Parts we currently have

ToeHold Exchange Questions

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Wild Type Huntinton mRNA - GBlock Aligned

What did our PCR actually amplify?

Wild Type Huntinton mRNA - GBlock Aligned

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HD RESOLUTION