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− | <!-- Generator: Sketch 3.6.1 (26313) - http://www.bohemiancoding.com/sketch -->
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− | <title>sequence</title>
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− | <desc>Created with Sketch.</desc>
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− | <path d="M0,8 C0,12.418278 3.581722,16 8,16 C12.418278,16 16,12.418278 16,8 C16,3.92038235 12.9463116,0.553991843 9,0.0618937549 L9,1.82956975 C11.9764258,2.30820976 14.25,4.88859403 14.25,8 C14.25,11.4517797 11.4517797,14.25 8,14.25 C4.54822031,14.25 1.75,11.4517797 1.75,8 C1.75,4.88859403 4.02357418,2.30820976 7,1.82956975 L7,0.0618937549 C3.05368842,0.553991843 0,3.92038235 0,8 Z" id="sequence_b33ff1e4a9d7f262694f1b0d0b74a27b_Combined-Shape"></path>
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− | <div class="printable-container hide-print-links">
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− | <div class="printable-page-container">
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− | <div class="noteEditor mediocre" tabindex="-1">
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− | <div></div>
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− | <div class="noteEditor-container">
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− | <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container">
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− | <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1">
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− | <h1 class="mediocre-titleEditor">
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− | <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Final days time calculaton: How Much Time do we need?</div>
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− | <span></span>
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− | </h1>
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− | <div>
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− | <div><b>Project:</b> HD Resolution Shared Project</div>
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− | <div><b>Authors:</b> jason fuller</div>
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− | <div><b>Date:</b> 2017-10-25</div>
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− | </div>
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− | <div class="editor" data-editor="notes">
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− | <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div>
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− | <div class="daySeparator" data-scroll-id="entryDay1432">
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− | <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div>
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− | <span class="daySeparator-date">Wednesday, 10/25/17</span>
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− | <div class="daySeparator-line"></div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1436">
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− | <div class="mediocre-noteEditable multi-editable is-header2">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1436" data-index="0" style="margin-left:0;">Working Backwards:</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1437">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1437" data-index="1" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1438">
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− | <div class="mediocre-noteEditable multi-editable is-subheader1">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1438" data-index="2" style="margin-left:0;">Shipping the part: (7 hrs)</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1439">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1439" data-index="3" style="margin-left:0;">MiniPrep the Plasmid out of the cells = 1 hr</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1440">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1440" data-index="4" style="margin-left:0;">Dry the DNA (in 2 fractions) = 30 min</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1441">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1441" data-index="5" style="margin-left:0;">Reconstitute 1 of 2 fractions in water and take OD reading 10 min</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1442">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1442" data-index="6" style="margin-left:0;">Reconsititue remaning fration in ethanol to a concentration of 25ng/ul and transfer 10ul to shipping plate, then drying = 4-6 hrs </div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1443">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1443" data-index="7" style="margin-left:0;">(I think we can do this in 1)</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1444">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1444" data-index="8" style="margin-left:0;">Add foil seal, pack, and get to a shipper 20 min</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1445">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1445" data-index="9" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1446">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1446" data-index="10" style="margin-left:0;"><u>Unplanned Gotchas:</u></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1447">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1447" data-index="11" style="margin-left:0;">Guessing the pakage weight and getting and printing a shipping label with tracking number</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1448">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1448" data-index="12" style="margin-left:0;">People using the nanodrop when we go to take OD readings</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1449">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1449" data-index="13" style="margin-left:0;">Genewiz shipping number and sample tracking info needed to be submitted along with part before this point!</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1450">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1450" data-index="14" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1451">
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− | <div class="mediocre-noteEditable multi-editable is-subheader1">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1451" data-index="15" style="margin-left:0;">Cell Culture outgrowth: (19hrs) - 26</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1452">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1452" data-index="16" style="margin-left:0;">Pick colonies (that glow? that don't glow? how can we ensure we have the right plasmid?): 1 hr</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1453">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1453" data-index="17" style="margin-left:0;">Grow cultures over night: 16- 20 hrs</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1454">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1454" data-index="18" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1455">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1455" data-index="19" style="margin-left:0;"><u>Unplanned Gotchas:</u></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1456">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1456" data-index="20" style="margin-left:0;">Not having sterlilized Chloramphenical containing media</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1457">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1457" data-index="21" style="margin-left:0;">Colonys too small</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1458">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1458" data-index="22" style="margin-left:0;">Colonies hard to differentatiate (no flourescense? - add 2 hrs?)</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1459">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1459" data-index="23" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1460">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1460" data-index="24" style="margin-left:0;"><b>Transform and Grow Colonies (19 hrs) - 45</b></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1461">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1461" data-index="25" style="margin-left:0;">Prep and Transform colonies with Plasmid containing our Part: 1 hr</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1462">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1462" data-index="26" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Grow colonies over night: 16- 20 hr</span></span></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1463">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1463" data-index="27" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1464">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1464" data-index="28" style="margin-left:0;">Ligate part into backbone and isolate</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1465">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1465" data-index="29" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1466">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1466" data-index="30" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1467">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1467" data-index="31" style="margin-left:0;">Order Oligos</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1468">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1468" data-index="32" style="margin-left:0;">Arrive noon tomorrow: 28 HRs 28 +45 = 6</div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1469">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1469" data-index="33" style="margin-left:0;"></div>
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− | </div>
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− | </div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1470">
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1471" data-index="35" style="margin-left:0;"><b>Notes:</b></div>
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− | <div class="mediocre-item is-text" data-scroll-id="note-part1472">
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− | <div class="mediocre-noteEditable multi-editable is-paragraph">
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− | <div class="mediocre-noteEditable-indenter" style="width:0;"></div>
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− | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1472" data-index="36" style="margin-left:0;">Separating supercoild plasmid dna and nicked plasmids from linear can be done using λ endonuclease (it can't restrict nicked plasmids) and <span style="color:#000000"><span style="background-color:#FFFFFF">RecJ</span></span><sub><span style="color:#000000"><span style="background-color:#FFFFFF">f </span></span></sub> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832929/</div>
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| + | <div> |
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| + | <desc>Created with Sketch.</desc> |
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| + | </svg> |
| + | <div> |
| + | <div class="printable-container hide-print-links"> |
| + | <div> |
| + | <div class="printable-page-container"> |
| + | <div class="noteEditor mediocre" tabindex="-1"> |
| + | <div></div> |
| + | <div class="noteEditor-container"> |
| + | <div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"> |
| + | <div class="noteEditor-contentWrapper" data-mediocre-item-whitespace="1"> |
| + | <h1 class="mediocre-titleEditor"> |
| + | <div class="mediocre-titleEditor-titleEditable editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Final days time calculaton: How Much Time do we need?</div> |
| + | <span></span> |
| + | </h1> |
| + | <div> |
| + | <div><b>Project:</b> HD Resolution Shared Project</div> |
| + | <div><b>Authors:</b> jason fuller</div> |
| + | <div><b>Date:</b> 2017-10-25</div> |
| + | </div> |
| + | <div class="editor" data-editor="notes"> |
| + | <div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div> |
| + | <div class="daySeparator" data-scroll-id="entryDay1432"> |
| + | <div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div> |
| + | <span class="daySeparator-date">Wednesday, 10/25/17</span> |
| + | <div class="daySeparator-line"></div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1436"> |
| + | <div class="mediocre-noteEditable multi-editable is-header2"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1436" data-index="0" style="margin-left:0;">Working Backwards:</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1437"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1437" data-index="1" style="margin-left:0;"></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1438"> |
| + | <div class="mediocre-noteEditable multi-editable is-subheader1"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1438" data-index="2" style="margin-left:0;">Shipping the part: (7 hrs)</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1439"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1439" data-index="3" style="margin-left:0;">MiniPrep the Plasmid out of the cells = 1 hr</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1440"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1440" data-index="4" style="margin-left:0;">Dry the DNA (in 2 fractions) = 30 min</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1441"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1441" data-index="5" style="margin-left:0;">Reconstitute 1 of 2 fractions in water and take OD reading 10 min</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1442"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1442" data-index="6" style="margin-left:0;">Reconsititue remaning fration in ethanol to a concentration of 25ng/ul and transfer 10ul to shipping plate, then drying = 4-6 hrs </div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1443"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1443" data-index="7" style="margin-left:0;">(I think we can do this in 1)</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1444"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1444" data-index="8" style="margin-left:0;">Add foil seal, pack, and get to a shipper 20 min</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1445"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1445" data-index="9" style="margin-left:0;"></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1446"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1446" data-index="10" style="margin-left:0;"><u>Unplanned Gotchas:</u></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1447"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1447" data-index="11" style="margin-left:0;">Guessing the pakage weight and getting and printing a shipping label with tracking number</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1448"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1448" data-index="12" style="margin-left:0;">People using the nanodrop when we go to take OD readings</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1449"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1449" data-index="13" style="margin-left:0;">Genewiz shipping number and sample tracking info needed to be submitted along with part before this point!</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1450"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1450" data-index="14" style="margin-left:0;"></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1451"> |
| + | <div class="mediocre-noteEditable multi-editable is-subheader1"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1451" data-index="15" style="margin-left:0;">Cell Culture outgrowth: (19hrs) - 26</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1452"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1452" data-index="16" style="margin-left:0;">Pick colonies (that glow? that don't glow? how can we ensure we have the right plasmid?): 1 hr</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1453"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1453" data-index="17" style="margin-left:0;">Grow cultures over night: 16- 20 hrs</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1454"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1454" data-index="18" style="margin-left:0;"></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1455"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1455" data-index="19" style="margin-left:0;"><u>Unplanned Gotchas:</u></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1456"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1456" data-index="20" style="margin-left:0;">Not having sterlilized Chloramphenical containing media</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1457"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1457" data-index="21" style="margin-left:0;">Colonys too small</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1458"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1458" data-index="22" style="margin-left:0;">Colonies hard to differentatiate (no flourescense? - add 2 hrs?)</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1459"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1459" data-index="23" style="margin-left:0;"></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1460"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1460" data-index="24" style="margin-left:0;"><b>Transform and Grow Colonies (19 hrs) - 45</b></div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1461"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1461" data-index="25" style="margin-left:0;">Prep and Transform colonies with Plasmid containing our Part: 1 hr</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1462"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1462" data-index="26" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Grow colonies over night: 16- 20 hr</span></span></div> |
| + | </div> |
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| + | <div class="mediocre-item is-text" data-scroll-id="note-part1463"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
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| + | </div> |
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| + | <div class="mediocre-item is-text" data-scroll-id="note-part1464"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1464" data-index="28" style="margin-left:0;">Ligate part into backbone and isolate</div> |
| + | </div> |
| + | </div> |
| + | <div class="mediocre-item is-text" data-scroll-id="note-part1465"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
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| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1466" data-index="30" style="margin-left:0;"></div> |
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| + | <div class="mediocre-item is-text" data-scroll-id="note-part1467"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1467" data-index="31" style="margin-left:0;">Order Oligos</div> |
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| + | <div class="mediocre-item is-text" data-scroll-id="note-part1468"> |
| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
| + | <div class="mediocre-noteEditable-indenter" style="width:0;"></div> |
| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1468" data-index="32" style="margin-left:0;">Arrive noon tomorrow: 28 HRs 28 +45 = 6</div> |
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| + | <div class="mediocre-item is-text" data-scroll-id="note-part1471"> |
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| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1471" data-index="35" style="margin-left:0;"><b>Notes:</b></div> |
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| + | <div class="mediocre-noteEditable multi-editable is-paragraph"> |
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| + | <div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1472" data-index="36" style="margin-left:0;">Separating supercoild plasmid dna and nicked plasmids from linear can be done using λ endonuclease (it can't restrict nicked plasmids) and <span style="color:#000000"><span style="background-color:#FFFFFF">RecJ</span></span><sub><span style="color:#000000"><span style="background-color:#FFFFFF">f </span></span></sub> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832929/</div> |
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