Revathireddy (Talk | contribs) |
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<h2>Week 4:The idea!</h2> | <h2>Week 4:The idea!</h2> | ||
− | <p style="font-family: 'Lato'; font-size:15px">Glutamine synthetase (<i>glnA</i>), smaller and bigger subunits of glutamate synthetase (<i>gltD</i> and <i>gltB</i> respectively) were our genes of primary interest. All the three genes of interest were amplified from <i> E. coli </i>, BW25113 genomic DNA using polymerase chain reaction (PCR).PCR products were loaded and run on 1% agarose gel to check for the correct sizes of these three genes (<i>glnA</i>:1640bp,<i>gltB:4462bp</i>, <i>gltD</i>:1420bp). Among the three, we were successful in obtaining two genes - <i>glnA</i> and <i>gltD</i>. We repeated PCR for <i>gltB</i> for the second time by adding DMSO. This | + | <p style="font-family: 'Lato'; font-size:15px">Glutamine synthetase (<i>glnA</i>), smaller and bigger subunits of glutamate synthetase (<i>gltD</i> and <i>gltB</i> respectively) were our genes of primary interest. All the three genes of interest were amplified from <i> E. coli </i>, BW25113 genomic DNA using polymerase chain reaction (PCR).PCR products were loaded and run on 1% agarose gel to check for the correct sizes of these three genes (<i>glnA</i>:1640bp,<i>gltB:4462bp</i>, <i>gltD</i>:1420bp). Among the three, we were successful in obtaining two genes - <i>glnA</i> and <i>gltD</i>. We repeated PCR for <i>gltB</i> for the second time by adding DMSO. This time too we could not see bands of correct size. |
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Revision as of 08:49, 31 October 2017