Difference between revisions of "Team:ICT-Mumbai/Safety"

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{{ICT-Mumbai}}
 
 
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<title>ICT-Mumbai 2017</title>
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<h1> Safety </h1>
 
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Background" class="w3-bar-item w3-button">Background</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Description" class="w3-bar-item w3-button">Description</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Design" class="w3-bar-item w3-button">Design</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Notebook"class="w3-bar-item w3-button">Notebook</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Protocols"class="w3-bar-item w3-button">Protocols</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/InterLab"class="w3-bar-item w3-button">InterLab</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Results"class="w3-bar-item w3-button">Results</a>
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<li>Choosing a non-pathogenic chassis</li>
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<a href="#team" button class="w3-button" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-users" aria-hidden="true"></i>Team</button></a>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Team"class="w3-bar-item w3-button">Team</a>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Collaborations" class="w3-bar-item w3-button">Collaborations</a>
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<a href="https://2017.igem.org/Team:ICT-Mumbai/Safety" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-flag" aria-hidden="true"></i>
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<h5>Safe Lab Work</h5>
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Safety</a>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<a href="https://2017.igem.org/Team:ICT-Mumbai/HP/Gold_Integrated" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-handshake-o" aria-hidden="true"></i>
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Human Practices</a>
  
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<a href="https://2017.igem.org/Team:ICT-Mumbai/Attributions" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-user-o" aria-hidden="true"></i>Attributions</a>
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    <a href="#about" class="w3-bar-item w3-button" onclick="toggleFunction()">ABOUT</a>
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    <a href="#portfolio" class="w3-bar-item w3-button" onclick="toggleFunction()">PORTFOLIO</a>
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<h5>Safe Shipment</h5>
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  <!--<h3 class="w3-center" style="font-weight: bold">Team ICT-Mumbai 2017</h3>
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  <p style="font-size:20px; font-family:'Lato', sans-serif; colour:lightgrey;">Ammonia released due to hydrolysis of urine is a major reason for the stench in public toilets. Apart from being a put-off, ammonia is also hazardous. Current approaches to tackle this problem include flushing water, using microbes (for example, BioBlocks®) that break down urea in urine to prevent its hydrolysis, and using air fresheners. The first approach requires using copious amounts of water, which is not abundantly available in many parts of the world, while the second is not cost-effective. Finally, spraying air fresheners or using deodorizers is not a solution, as it does not get rid of ammonia. We propose to engineer <i>Escherichia coli</i> to assimilate ammonia and convert it into indigoidine, a blue coloured compound. Ammonia from air can be dissolved in an aqueous medium and can act as a nitrogen source for the engineered cells that can be housed in a cassette. The synthesized indigoidine will indicate when the cassette has to be replaced.</p>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
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Revision as of 09:53, 31 October 2017

ICT-Mumbai 2017

Ammonia released due to hydrolysis of urine is a major reason for the stench in public toilets. Apart from being a put-off, ammonia is also hazardous. Current approaches to tackle this problem include flushing water, using microbes (for example, BioBlocks®) that break down urea in urine to prevent its hydrolysis, and using air fresheners. The first approach requires using copious amounts of water, which is not abundantly available in many parts of the world, while the second is not cost-effective. Finally, spraying air fresheners or using deodorizers is not a solution, as it does not get rid of ammonia. We propose to engineer Escherichia coli to assimilate ammonia and convert it into indigoidine, a blue coloured compound. Ammonia from air can be dissolved in an aqueous medium and can act as a nitrogen source for the engineered cells that can be housed in a cassette. The synthesized indigoidine will indicate when the cassette has to be replaced.