Difference between revisions of "Team:ICT-Mumbai/Safety"

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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal.</p>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal.<br>
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Figures<br>
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We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.</p>
  
 
      
 
      

Revision as of 10:50, 31 October 2017

ICT-Mumbai 2017

Our host organism

The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ Escherichia coli. We have used different strains of E. coli for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised under Biosafety Level I (BSL I) and safe to use and handle.

Safe practices in our laboratory

We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal.
Figures
We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.