Revathireddy (Talk | contribs) |
Revathireddy (Talk | contribs) |
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<p style="font-family: 'Lato'; font-size:15px">We reconstituted BBa_K773002 (Plate 2, well 1P), BBa_K1604022 (Plate 7, well 17K), BBa_R0080 (Plate 3, well 5G) and transformed in <i>E. coli</i> DH5-α competent cells.</p> | <p style="font-family: 'Lato'; font-size:15px">We reconstituted BBa_K773002 (Plate 2, well 1P), BBa_K1604022 (Plate 7, well 17K), BBa_R0080 (Plate 3, well 5G) and transformed in <i>E. coli</i> DH5-α competent cells.</p> | ||
<p style="font-family: 'Lato'; font-size:15px">Colonies were found only for BBa_K773002. BBa_K1604022 and BBa_R0080 gave no colonies. We repeated the transformation for these two parts, this time we increased the incubation time for each. We were successful.</p> | <p style="font-family: 'Lato'; font-size:15px">Colonies were found only for BBa_K773002. BBa_K1604022 and BBa_R0080 gave no colonies. We repeated the transformation for these two parts, this time we increased the incubation time for each. We were successful.</p> | ||
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+ | <div class="container left"> | ||
+ | <div class="content"> | ||
+ | <h2>Week 9:Eureka moments!</h2> | ||
+ | <p style="font-family: 'Lato'; font-size:15px">We learned that farnesyl diphosphate (FPP) an intermediate in the carotene biosynthesis pathway is is also the starting molecule for a fragrant compound, limonene. Catalysed by limonene synthase (LIMS), FPP is converted to limonene. To our surprise we found the gene coding for LIMS+rbs (BBa_I74211) is present in the kitplate. We planned to clone this part downstream of BBa_K1604022 so as to obtain limonene. Thus, we reconstituted and transformed this part from well 4I of plate 4 in <i>E. coli</i> DH5-α competent cells. Colonies obtained were inoculated for making glycerol stocks and miniprepped the next day.</p> | ||
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Revision as of 18:56, 31 October 2017