Difference between revisions of "Team:Dalhousie/Results"

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<font color= "#C1D35D">DNA Extraction</font> </br></br>
 
<font color= "#C1D35D">DNA Extraction</font> </br></br>
  
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We were able to confirm the size of the metagenomic DNA isolated from the porcupine fecal samples (Fig.1). The remainder of the DNA was ran via PFGE and all DNA from 24.8 kB was excised without exposure to UV or ethidium bromide. The gel was stained after words for visualization (Fig. 2).</br>
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We were able to confirm the size of the metagenomic DNA isolated from the porcupine fecal samples via pulse field gel electrophoresis (PFGE) (Fig.1). The remainder of the DNA was ran via PFGE and all DNA larger than 24.8 kB was excised without exposure to UV or ethidium bromide. The gel was stained after words for visualization (Fig. 2).</br></br>
  
 
<font color= "#C1D35D">Vector Preparation</font> </br></br>
 
<font color= "#C1D35D">Vector Preparation</font> </br></br>

Revision as of 20:28, 31 October 2017

Results

Results


Metagenomic Library


DNA Extraction

We were able to confirm the size of the metagenomic DNA isolated from the porcupine fecal samples via pulse field gel electrophoresis (PFGE) (Fig.1). The remainder of the DNA was ran via PFGE and all DNA larger than 24.8 kB was excised without exposure to UV or ethidium bromide. The gel was stained after words for visualization (Fig. 2).

Vector Preparation

Trouble-Shooting

Future Directions of the cosmid library

Sequencing Meagenomic and Cloning


Project Achievements



Over the course of the 2017 iGEM season, we have had some downs, but many more ups.
Successes
  • Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.
  • Identified 8? novel enzymes with variable percent identity.
  • Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.
  • Optimized our previous biobrick Endoglucanse(BBa_K2160000)by adding a C termincal HIS-tag and N terminal PelB sequence (Improve).
  • Successfully completed a fluorophore cleavage assay from the Hallam lab.
  • Isolated high molecular weight DNA from porcupine fecal samples.
  • Obtained efficient ligation and digestion with pJC8 controls.
  • Produced phage plaque with the phage packaging extract lamba DNA controls.
Failures
  • Did not clone our biobricks into the shipping vector psB1C3
  • Was not able to achieve the right environment for our novel beta-xylanase to function
  • Was not able to design a functional media assay for our enzymes
  • Could not make a functional metagenomic library
  • Sheered our high molecular weight DNA