Difference between revisions of "Team:Vilnius-Lithuania"

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                         <div class="step">1. 3.</div>
 
                         <div class="step">1. 3.</div>
 
                         <div class="title">Plasmid loss</div>
 
                         <div class="title">Plasmid loss</div>
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                         <p> This is even more likely to occur when the plasmid copy number is low. When the cell
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                         <p> This is even more likely to occur when one plasmid is low copy number. When the cell
−
                             divides, lower copy plasmids tend to be lost in next generations. </p>
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                             divides, low copy plasmids tend to be lost in next generations. </p>
 
                         <h6>Problem #1</h6>
 
                         <h6>Problem #1</h6>
 
                     </div>
 
                     </div>

Revision as of 21:39, 31 October 2017

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Present challenges of
experimenting with plasmid systems

1. 1.
Plasmid replication

Origin of replication (Ori) is a sequence where the plasmid replication is initiated. Plasmids with similar Ori sites use the same replication machinery.

Problem #1
1. 2.
Unequal replication

Replication machinery is not able to discriminate between similar Ori sites, therefore smaller plasmids are replicated faster, thus rapidly outgrowing larger plasmids leading to uneven plasmid ratios in a cell.

Problem #1
1. 3.
Plasmid loss

This is even more likely to occur when one plasmid is low copy number. When the cell divides, low copy plasmids tend to be lost in next generations.

Problem #1
2. 1.
Plasmid incompatibility

One needs to use different types of Ori to avoid this trouble, however it often causes dilemma due to limited selection options or the lack of good combination of Ori and plasmid‘s copy number.

Problem #2
2. 2.
Pavadinimas

Imagine you require a system consisting of two - low and high copy plasmids. Try picking them from the list above and see what happens.

Problem #2
2. 3.
Pavadinimas

Currently no handy method of changing the copy number of particular plasmid exists.

Problem #2
2. 4.
Pavadinimas

There is also no mechanism to adjust the copy number when so is required in any specific situation: for example, incoming cellular signals or special patterns.

Problem #2

Did this look smart, easy or convenient?

We think it is time for a change.

Plasmid A
Plasmid B
Origin of replication
Replication machinery
Plasmid A
Plasmid B
Origin of replication
Replication machinery
Plasmid A
Plasmid B
Origin of replication
Replication machinery
Different plasmids

A

B

C

D

Different types of ORI
Different replication machinery molecules

Plasmid

Copy number

A
High
B
High
C
Medium
D
Low
E
Low
High
Medium
Low
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