Difference between revisions of "Team:Amazonas Brazil/Project"
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<h3>Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR</h3> | <h3>Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR</h3> | ||
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| − | + | The scientific community had achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some basic methodological aspects related to this approach could be further improved to expand this scientific revolution. | |
| − | + | In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite laborious days of work, the system is weakly founded on bioengineering principles, demands lots of steps, each one carrying a part of the CRISPR/Cas9 apparatus. In addition, off-target events happen frequently, the machinery post-effect in the cell isn’t entirely known yet, among others. To legitimate this information, we integrated our project design with other iGEM's teams who had worked with CRISPR/Cas9 in previous years. You can read more about what we found here (LINK PRA INTEGRADA) | |
| − | + | The decoupling of all these shortcomings paved the way to the project's delineation and we elected to focus on a challenge: the lack of standardization, which is a real-world problem - included but not limited to SynBio. Then, to optimize the previous methods, we designed a trustworthy, well functioning standardized framework based on BioBrick parts called: the CRISPeasy toolbox. | |
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Revision as of 02:44, 1 November 2017
Navigate into CRISPeasy roadmap
Specifications
Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR
The scientific community had achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some basic methodological aspects related to this approach could be further improved to expand this scientific revolution. In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite laborious days of work, the system is weakly founded on bioengineering principles, demands lots of steps, each one carrying a part of the CRISPR/Cas9 apparatus. In addition, off-target events happen frequently, the machinery post-effect in the cell isn’t entirely known yet, among others. To legitimate this information, we integrated our project design with other iGEM's teams who had worked with CRISPR/Cas9 in previous years. You can read more about what we found here (LINK PRA INTEGRADA) The decoupling of all these shortcomings paved the way to the project's delineation and we elected to focus on a challenge: the lack of standardization, which is a real-world problem - included but not limited to SynBio. Then, to optimize the previous methods, we designed a trustworthy, well functioning standardized framework based on BioBrick parts called: the CRISPeasy toolbox.
Design
Check all the parts involved and our approaches with closer details!
Cas9 (BBa_K2457001): Standardized Cas9 device, regulated by the AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair pathways, it will lays the road to genomic editing in living cells.
