Revathireddy (Talk | contribs) |
Revathireddy (Talk | contribs) |
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<h2>Week 22: Our part is ready!</h2> | <h2>Week 22: Our part is ready!</h2> | ||
<p style="font-family: 'Lato'; font-size:15px"> Screening results for all eight colonies were positive!<br><br> | <p style="font-family: 'Lato'; font-size:15px"> Screening results for all eight colonies were positive!<br><br> | ||
− | <img src="https://static.igem.org/mediawiki/parts/ | + | <img src="https://static.igem.org/mediawiki/parts/f/f6/ICT-Mumbai_notebook_gelimage8.png"><br> |
From left to right: Gel1_Lanes 1-5: Colony number 1-5, lane 7: Positive control, lane 8: 1kb DNA ladder, Gel2_Lanes 1-3: Colony number 6-8, lane 7: Positive control, lane 8: 1kb DNA ladder. We determined the concentration of each of these using Nanodrop spectrophotometer.<br><br> | From left to right: Gel1_Lanes 1-5: Colony number 1-5, lane 7: Positive control, lane 8: 1kb DNA ladder, Gel2_Lanes 1-3: Colony number 6-8, lane 7: Positive control, lane 8: 1kb DNA ladder. We determined the concentration of each of these using Nanodrop spectrophotometer.<br><br> | ||
Submission: Based on the concentration obtained, we added 11ul of miniprepped DNA prep (Colony 1: pSB1C3+ychH promoter) to A1 of the 96 well submission plate, covered it with the provided lid and kept it for overnight drying. Drying was carried out in the lab’s laminar flow hood which was surface sterilized with 90% ethanol. The miniprepped DNA had completely dried in the wellthe next day. We submitted our part as per the instructions given on the <a href="http://parts.igem.org/Help:Submission_Kit"> iGem submission page</a> | Submission: Based on the concentration obtained, we added 11ul of miniprepped DNA prep (Colony 1: pSB1C3+ychH promoter) to A1 of the 96 well submission plate, covered it with the provided lid and kept it for overnight drying. Drying was carried out in the lab’s laminar flow hood which was surface sterilized with 90% ethanol. The miniprepped DNA had completely dried in the wellthe next day. We submitted our part as per the instructions given on the <a href="http://parts.igem.org/Help:Submission_Kit"> iGem submission page</a> |
Revision as of 04:47, 1 November 2017