Revision as of 04:48, 1 November 2017

In a controlled environment, neurons with enhanced olfactory receptors are cultured on a microelectrode array. While media is flowed through via a peristaltic pump, certain olfactants are injected. When the neurons detect these olfactants, they produce an electrical signal, which is then transferred to a computer for interpretation.

After conducting multiple trials, it was shown that there was no significant increase in voltage reading the olfactant was introduced into the closed system. This may have been because the plasmid is not being expressed sufficiently, or that 24 hours after transfection was not the optimal time to conduct trials. There was also no reference electrode, which could have altered the computers interpretation of the voltage data

TEL: 845.938.3915

Difference between revisions of "Team:USMA-West Point/Hardware"

Revision as of 04:48, 1 November 2017

USMA-West_Point

usMA-WEST POINT

hardware

Handheld Photosensor Device

Microelectrode Array Bioreactor

DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE

BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA

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 {{USMA-West_Point}}
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-		<p><span style="color: rgb(0, 0, 0);">The Electronic Nose (eNose) is composed of 1st Class Cadets, 2nd Class Cadets, and 3rd Class Cadets in the Departments of Chemistry and Life Science and Department of Mathematical Sciences. We've partnered with scientists from the Naval Research Laboratory (NRL) to develop a synthetic biology project to assist with the detection of aerosols and gases using a cell-based sensor.</span></p><p><br></p><p><span style="color: rgb(0, 0, 0);">Located in the scenic Hudson River Valley, only 50 miles north of New York City, the U.S. Military Academy at West Point is a four-year academic institution, a nationally-renowned historic site, and the oldest continuously occupied military post in America.</span></p>
+		<p class="font_8" style="line-height: 1.5em;">Two prototypes have been designed and developed capable of utilizing different methods of biosensor detection. One of the devices utilizes a photo sensor connected to an Arduino, translating the cell action potentials into plain text. <br></p>
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+		<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Two prototypes have been designed and developed capable of utilizing different methods of biosensor detection. One of the devices utilizes a photo sensor connected to an Arduino, translating the cell action potentials into plain text. </span><br></p>
-	<div id="vbid-7d38c6a1-3phnff2l" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
-		<p class="font_8" style="text-align: center; line-height: 1.5em;"><span style="color:#000000;">Our team is a highly trained collection of Life Science Cadets from the United States Military Academy. We are leaders at West Point, and we plan to be leaders of science in synthetic biology.<br></span></p>
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-		<p><span style="color:#000000;"><span class="color_11">Our project utilizes the natural genetic sequence of human olfactory receptors. These plasmid sequences are inserted into animal neurons and a measured action potential can be used to detect odorants for homeland security, medical, and other necessary applications.</span></span><br></p>
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-		<p><span style="color:#000000;"><span class="color_11">We accomplished the necessary requirements for the bronze and silver iGEM medals. Judge us for Gold. Click here to learn more.</span></span><br></p>
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+		<p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);">In a controlled environment, neurons with enhanced olfactory receptors are cultured on a microelectrode array. While media is flowed through via a peristaltic pump, certain olfactants are injected. When the neurons detect these olfactants, they produce an electrical signal, which is then transferred to a computer for interpretation.</span></p><p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);"><span class="wixGuard"></span></span></p><p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);">After conducting multiple trials, it was shown that there was no significant increase in voltage reading the olfactant was introduced into the closed system. This may have been because the plasmid is not being expressed sufficiently, or that 24 hours after transfection was not the optimal time to conduct trials. There was also no reference electrode, which could have altered the computers interpretation of the voltage data</span><br></p>
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-<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
-	</div>
-</div>
-				</div>
-			</div>
-				<div class="text-side shrinker-parent">
-					<div class="vertical-aligner">
-						<div class="item-details preview-content-wrapper  multi" style="position:relative;">
-							<div class="draggable-div-holder"></div>
-							<div class="preview-content-holder shrinker-content">
-										<!--  BY NATIVE ORDER -->
-	<div class="preview-icon-holder Icon removable-parent order-handle" >
-		<img id="vbid-7d38c6a1-slldoddp" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://lh3.googleusercontent.com/JT-jn3bKsEqGBbHZxyh7zHyataNlhtRTi60Mmq6CRLR0adnX59-XatcbJk5zeng3Gzbkwyk-IqEzizX0uw" />
-	</div>
-<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
-											<div class="preview-form order-handle">
-<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
-</div>
-											<div class="preview-item-links order-handle ">
-	<div class="preview-links-wrapper">
-<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
-	</div>
-</div>
-<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
-							</div>
-						</div>
-					</div>
-				</div>
-			</div>
-		</div>
-	</div>
-</div>
-</div>
-<!-- LAYOUT SETTINGS -->
-<div class="layout-settings" style="display:none;" data-type="multi"></div>
-			</div>
-		</div>
-		<div  id="vbid-7d38c6a1-mst181pj"  class="sub item-box  page-box style-7d38c6a1-ibikj8su          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-7d38c6a1-ibikj8su" data-preview-styleid='style-7d38c6a1-ibikj8su' data-preset-type-id="UNRESOLVED">
-			<div class="page-wrapper item-wrapper ">
-					<div class="item-content leaf multi_layout page content -container" data-self="vbid-7d38c6a1-mst181pj" data-preview-style="style-7d38c6a1-ibikj8su" data-style="style-bcb38-bwz05fysvv" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-7d38c6a1-mst181pj" data-bgimg="">
-<div  class="multi-container preview image-cover" >
-	<div class="Picture item-preview">
-		<div class="preview-image-holder">
-			<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
-			</div>
-			<div class="helper-div middle-center" >
-			<!-- <div class="benet" style="min-height:inherit;"></div> -->
-			<div class="pic-side">
-				<div class="vertical-aligner">
-					<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
-	<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
-		<!-- VIDEO TEMPLATE -->
-<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
-		<!-- MAP TEMPLATE -->
-<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
-		<!-- RAW TEMPLATE -->
-<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
-	</div>
-</div>
-				</div>
-			</div>
-				<div class="text-side shrinker-parent">
-					<div class="vertical-aligner">
-						<div class="item-details preview-content-wrapper  multi" style="position:relative;">
-							<div class="draggable-div-holder"></div>
-							<div class="preview-content-holder shrinker-content">
-										<!--  BY NATIVE ORDER -->
-	<div class="preview-icon-holder Icon removable-parent order-handle" >
-		<img id="vbid-7d38c6a1-c8tbuuyr" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://lh3.googleusercontent.com/_YEpqPLcBzYjDQ1NmuaizcOER6J7dwg1g1P3KYNPZtiNYhene9N9FN6HI8STZDL2PY0gO8jpRgWK4vTALw" />
-	</div>
-<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
-<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
-											<div class="preview-form order-handle">
-<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
-</div>
-											<div class="preview-item-links order-handle ">
-	<div class="preview-links-wrapper">
-<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
-	</div>
-</div>
-<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
@@ Line 1,560: / Line 1,006: @@
 	</div>
 </div>
-			</div>
-		</div>
-		<!-- FEATURES END -->
-	<!-- TEXT_BLOCK START -->
-		<div  id="vbid-7d38c6a1-sweqbjtx"  class="master item-box  page-box style-7d38c6a1-i1eetr8y          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-7d38c6a1-i1eetr8y" data-preview-styleid='style-7d38c6a1-i1eetr8y' data-preset-type-id="TEXT_BLOCK">
-			<div id="no-image" class="stripe-background load-high-res " ></div>
-			<div class="page-wrapper item-wrapper text_block-wrapper ">
-					<div class="item-content leaf multi_layout page content -container" data-self="vbid-7d38c6a1-sweqbjtx" data-preview-style="style-7d38c6a1-i1eetr8y" data-style="style-53444-pkeicslc5r" data-orig-thumb-height="1303" data-orig-thumb-width="2000" data-vbid="vbid-7d38c6a1-sweqbjtx" data-bgimg="https://lh3.googleusercontent.com/I14iAhDtNTpAIix2chOQFewIKz5pUr3AONdYY2Aanop6bLO_GYYCYCv8f0Is24LLpHg82Sii9RUqmXH1">
-<div  class="multi-container preview image-cover" >
-	<div class="Picture item-preview">
-		<div class="preview-image-holder">
-			<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
-			</div>
-			<div class="helper-div middle-center" >
-			<!-- <div class="benet" style="min-height:inherit;"></div> -->
-			<div class="pic-side">
-				<div class="vertical-aligner">
-					<div id="vbid-7d38c6a1-lfdwpmrs-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
-	<div  id="vbid-7d38c6a1-lfdwpmrs" class="inner-pic preview-element parallax50-bg magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/I14iAhDtNTpAIix2chOQFewIKz5pUr3AONdYY2Aanop6bLO_GYYCYCv8f0Is24LLpHg82Sii9RUqmXH1=s300);"  data-orig-width="2000" data-orig-height="1303" >
-		<!-- VIDEO TEMPLATE -->
-<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
-		<!-- MAP TEMPLATE -->
-<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
-		<!-- RAW TEMPLATE -->
-<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
-	</div>
-</div>
-				</div>
-			</div>
-				<div class="text-side shrinker-parent">
-					<div class="vertical-aligner">
-						<div class="item-details preview-content-wrapper  multi" style="position:relative;">
-							<div class="draggable-div-holder"></div>
-							<div class="preview-content-holder shrinker-content">
-									<!--  BY SPECIFIC ORDER -->
-												<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
-		<a class="removable-parent" href="http://www.usma.edu/SitePages/Home.aspx"  data-link-type="EXTERNAL"   target="_self" >
-	<span id="vbid-7d38c6a1-hnu3c0ub"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >CONTACT US</span>
-</a>
-</div>
-							</div>
-						</div>
-					</div>
-				</div>
-			</div>
-		</div>
-	</div>
-</div>
-</div>
-<!-- LAYOUT SETTINGS -->
-<div class="layout-settings" style="display:none;" data-type="multi"></div>
 			</div>
@@ Line 1,668: / Line 1,015: @@
 	<!-- FOOTERS START -->
-		<div  id="vbid-7d38c6a1-vhgfqwrt"  class="master item-box  gallery-box style-7d38c6a1-sr6fbz1e          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-7d38c6a1-sr6fbz1e" data-preview-styleid='style-7d38c6a1-sr6fbz1e' data-preset-type-id="FOOTERS">
+		<div  id="vbid-7d38c6a1-vhgfqwrt"  class="master item-box  gallery-box style-7d38c6a1-sr6fbz1e injected         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="ORIGIN"  data-styleid="style-7d38c6a1-sr6fbz1e" data-preview-styleid='style-7d38c6a1-sr6fbz1e' data-preset-type-id="FOOTERS">
 			<div id="no-image" class="stripe-background load-high-res " ></div>
 			<div class="gallery-wrapper item-wrapper footers-wrapper ">