Difference between revisions of "Team:Emory/Description"
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| + | <p><font size="4" face="Georgia">The WaterHub at Emory University is a wastewater treatment plant on campus that utilizes bacteria and plants to recycle 400,000 gallons of water a day for non-potable use. Emory iGEM team is interested in using synthetic biology to help optimize their system to minimize maintenance processes. Currently, the WaterHub resorts to adding polyaluminum chloride to combat orthophosphate levels in some instances. The aim of our project is to engineer a bacteria that can combat their orthophosphate levels by creating a phosphate accumulating organism. By utilizing the Keio and ASKA <i>E. coli</i> collections, we plan to create an experimental strain of bacteria with high copy number plasmids of polyphosphate kinase and the exopolyphosphatase gene knocked out. We also plan to test the phosphate types and concentration levels within the WaterHub system. After engineering the bacteria, we hope to see the long-term efficiency of the cells’ phosphate uptake as well as survival within the WaterHub conditions. A secondary endeavor our team will pursue is the removal of excess calcium ions (Ca<sup>2+</sup>) from the water by exploring the potential of calmodulin-like proteins native to <i>E.coli</i>.</font></p> | ||
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Revision as of 18:08, 30 June 2017
Emory
The WaterHub at Emory University is a wastewater treatment plant on campus that utilizes bacteria and plants to recycle 400,000 gallons of water a day for non-potable use. Emory iGEM team is interested in using synthetic biology to help optimize their system to minimize maintenance processes. Currently, the WaterHub resorts to adding polyaluminum chloride to combat orthophosphate levels in some instances. The aim of our project is to engineer a bacteria that can combat their orthophosphate levels by creating a phosphate accumulating organism. By utilizing the Keio and ASKA E. coli collections, we plan to create an experimental strain of bacteria with high copy number plasmids of polyphosphate kinase and the exopolyphosphatase gene knocked out. We also plan to test the phosphate types and concentration levels within the WaterHub system. After engineering the bacteria, we hope to see the long-term efficiency of the cells’ phosphate uptake as well as survival within the WaterHub conditions. A secondary endeavor our team will pursue is the removal of excess calcium ions (Ca2+) from the water by exploring the potential of calmodulin-like proteins native to E.coli.
Description
Tell us about your project, describe what moves you and why this is something important for your team.
What should this page contain?
- A clear and concise description of your project.
- A detailed explanation of why your team chose to work on this particular project.
- References and sources to document your research.
- Use illustrations and other visual resources to explain your project.
Advice on writing your Project Description
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
References
iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.
Inspiration
See how other teams have described and presented their projects:
