Difference between revisions of "Team:Toronto/Hardware"

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<h2 class="text-red">General criteria considered when designing our devices</h2>
 
<h2 class="text-red">General criteria considered when designing our devices</h2>
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<li>A device that would deliver solely blue light at wavelength 465nm</li>
 
<li>A device that would deliver solely blue light at wavelength 465nm</li>
 
<li>A device that would insulate against ambient light and therefore minimize experimental error.</li>
 
<li>A device that would insulate against ambient light and therefore minimize experimental error.</li>
 
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A device that would be compatible with standard vessels used for experimentation
 
A device that would be compatible with standard vessels used for experimentation
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<li>15ml tubes</li>
 
<li>15ml tubes</li>
 
<li>60mm x 15mm petri plates</li>
 
<li>60mm x 15mm petri plates</li>

Revision as of 08:33, 1 November 2017

Hardware

Introduction

As soon as we decided to embark on a project that utilized the blue light sensitive LOV domain, it became clear that our project, as well as all future extensions of our project, would require some kind of hardware component. Over the course of the project, we developed and tested three different versions of our hardware device. V1.0 (Lionel), V2.0 (Ludwig). V3.0 (Loki). We briefly describe these devices, and provide information as to their purpose and usage, as well as design and assembly files - ultimately enabling future recapitulation of our work. Furthermore, we demonstrate how we incorporated user feedback as we progressed along our project - notably in the case of the design for V4.0 (Lancelot). In conjunction with our human practices initiative, we had the opportunity to interview various stakeholders and ask for feedback regarding our hardware, and about how our design could be altered in order to integrate it into gene editing applications.

General criteria considered when designing our devices

  • A device that would deliver solely blue light at wavelength 465nm
  • A device that would insulate against ambient light and therefore minimize experimental error.
  • A device that would be compatible with standard vessels used for experimentation
    • 15ml tubes
    • 60mm x 15mm petri plates
  • A device that could enable the user to easily regulate:
  • A device that allows for multiple samples to be housed in order to allow for appropriate experimental controls to be employed
  • A device that is compatible with growth conditions - this means that the device can be kept sterile, stored within incubators or orbital shakers, and should be usable at 37C
  • Fit within our budget constraints

Introduction

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Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population
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Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population

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Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population

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References

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General Pages

Wet Lab

Dry Lab

Human Practices

iGEM Toronto