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Revision as of 09:59, 1 November 2017

Team:Shenzhen SFLS/Demonstrate - 2017.igem.org

Team:Shenzhen SFLS/Experiment - 2017.igem.org

Help from Dr. Xiaoqiang Guo



During the experiment in our project, we encountered a problem of DNA sequencing. The result tented to be confusing. We couldn’t tell whether the mutant BRAF in melanoma cells had been cleaved. It seriously influenced our experiment process, so we asked for the experienced expert for help. Dr. Xiaoqiang Guo from Peking University suggested that we can avoid this by using TA cloning assay. With his help, we got satisfactory results.