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| <h2 class="major">Construct Model</h2> | | <h2 class="major">Construct Model</h2> |
− | <p>Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composing of fusion proteins as well as split luciferase fragments (COX-2 - nLuc - FRB and FKBP - cLuc - c-Myc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into E. coli cells. </p> | + | <p>Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composing of fusion proteins as well as split luciferase fragments (COX-2 - FRB - nLuc and c-Myc - FKBP - cLuc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into E. coli cells. </p> |
| </div> | | </div> |
| </div> | | </div> |
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| <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/9/90/P-Rsystemanchiegg.jpg" title="Click to return back to the top." /></a> | | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/9/90/P-Rsystemanchiegg.jpg" title="Click to return back to the top." /></a> |
| <h3 class="major">Figure 2</h3> | | <h3 class="major">Figure 2</h3> |
− | <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FKBP, c-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FRB, n-Luc, and terminator.</p> | + | <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FRB, n-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FKBP, c-Luc, and terminator.</p> |
| </article> | | </article> |
| <article> | | <article> |