Difference between revisions of "Team:Vilnius-Lithuania/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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                <svg class="shape-overlays" viewBox="0 0 100 100" preserveAspectRatio="none">
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                    <h1>Description</h1>
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                    <div class="category">Something</div>
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                    <p>Find out why plasmid copy number and multiple plasmid systems are so important, what is SynORI, how does it work, and how can you apply it.</p>
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                    <div class="readmore" data-modal="1">Read more</div>
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                </div>
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                <div class="slide-right-content slide-right-content-2 hidden">
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                    <h1>Overview of the project</h1>
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                    <div class="category">Students</div>
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                    <p>This is even more likely to occur when the plasmid copy number is low. When the cell divides, lower
 +
                        copy plasmids tend to be lost in next generations.</p>
 +
                    <div class="readmore" data-modal="2">read more</div>
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                </div>
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                <div class="slide-right-content slide-right-content-3 hidden">
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                    <h1>Trumpas title, per dvi eilutes</h1>
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                    <div class="category">Students</div>
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                    <p>This is even more likely to occur when the plasmid copy number is low. When the cell divides, lower
 +
                        copy plasmids tend to be lost in next generations.</p>
 +
                    <div class="readmore" data-modal="3">read more</div>
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                </div>
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                <div class="slide-right-content slide-right-content-4 hidden">
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                    <h1>Trumpas title, per dvi eilutes</h1>
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                    <div class="category">Students</div>
 +
                    <p>This is even more likely to occur when the plasmid copy number is low. When the cell divides, lower
 +
                        copy plasmids tend to be lost in next generations.</p>
 +
                    <div class="readmore" data-modal="4">read more</div>
 +
                </div>
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                <div class="slide-right-content slide-right-content-5 hidden">
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                    <h1>Trumpas title, per dvi eilutes</h1>
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                    <div class="category">Students</div>
 +
                    <p>This is even more likely to occur when the plasmid copy number is low. When the cell divides, lower
 +
                        copy plasmids tend to be lost in next generations.</p>
 +
                    <div class="readmore" data-modal="5">read more</div>
 +
                </div>
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                <div class="slide-right-content slide-right-content-6 hidden">
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                    <h1>Trumpas title, per dvi eilutes</h1>
 +
                    <div class="category">Students</div>
 +
                    <p>This is even more likely to occur when the plasmid copy number is low. When the cell divides, lower
 +
                        copy plasmids tend to be lost in next generations.</p>
 +
                    <div class="readmore" data-modal="6">read more</div>
 +
                </div>
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            </div>
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        </div>
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        <ul class="navigation">
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            <li><a href="/Team:Vilnius-Lithuania/Description" class="active">Description</a></li>
 +
            <li>
 +
                <a href="/Team:Vilnius-Lithuania/Design">Design</a>
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            </li>
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            <li><a href="/Team:Vilnius-Lithuania/Model">Modelling</a></li>
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            <li><a href="/Team:Vilnius-Lithuania/Results">Results</a></li>
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            <li><a href="/Team:Vilnius-Lithuania/Demonstrate">Proof of concept</a></li>
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            <li><a href="/Team:Vilnius-Lithuania/InterLab">Interlab</a></li>
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            <li><a href="/Team:Vilnius-Lithuania/Safety">Safety</a></li>
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        </ul>
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    </div>
 
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<ul>
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<div class="modal modal-1">
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<li>Choosing a non-pathogenic chassis</li>
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    <div class="modal-close"></div>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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    <div class="modal-content">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        <h1>Description</h1>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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        <p></p>
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</ul>
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      <p></p>
 +
        <h2>What is SynORI?</h2>
 +
        <p>SynORI stands for synthetic origin of replication. It is a framework designed to make working with single and multi-plasmid systems precise, easy and on top of that - more functional.</p>
 +
<p>In a standardized way, this system enables scientist to build a plasmid system by:</p>
 +
<ol>
 +
                      <li>Selecting the number of plasmid groups</li>
 +
                        <li>Picking the type of copy number control (specific to one group or regulating all of them at once)</li>
 +
                        <li>Choosing the copy number of each group.</li>
 +
                   
 +
                    </ol></p>
  
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</div>
+
      <p></p>
 +
      <p>The framework also comes with the possibility of adding a selection system that reduces the usage of antibiotics (only 1 antibiotic for up to 5 plasmids!) and an active partitioning systems to make sure low copy plasmid groups are not lost during division.
 +
</p>
 +
  <p></p>
 +
        <div class="img-cont">
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            <img src="https://static.igem.org/mediawiki/parts/8/84/Collect.png" alt="img">
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            </div>
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        </div>
 +
        <h2>Applications</h2>
 +
        <p>
 +
                        <h5>Everyday lab work</h5><p>
 +
Easy to assemble and control multi-plasmid system. With our framework there is no need to limit your research to a particular plasmid copy number just because there are no right replicons to choose from. With SynORI you can easily create a vector with a desired copy number that suits your needs.</li></p>
 +
                        <h5>Biological computing</h5><p>
 +
The ability to choose from a wide range of copy number options and their control type will make synthetic biology engineering much more flexible and predictable. Regulation of plasmid copy number is equivalent to adding a global parameter into a computer system. This enables the coordination of multiple gene group expression.
 +
</p>
 +
                        <h5>Smart assembly of large protein complexes</h5><p>
 +
Co-expression of multi-subunit complexes using different replicons brings incoherency to an already chaotic cell system. This can be avoided by using SynORI, as every plasmid group uses the same type of control, yet can act in a group-specific way.</p>
  
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<div class="column half_size">
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<h5>Metabolic engineering</h5><p>
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<h5>Safe Lab Work</h5>
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A big challenge for heterologous expression of multiple gene pathways is to accurately adjust the levels of each enzyme to achieve optimal production efficiency. Precise promoter tuning in transcriptional control and synthetic ribosome binding sites in translational control are already widely used to maintain expression levels. In addition to current approaches, our framework offers multiple gene control at the same time and on top of that, inducible regulation can make the search for perfect conditions a lot easier.
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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</div>
 
  
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<div class="column half_size">
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</p>
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<h5>Safe Shipment</h5>
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        <div class="plotly">
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 15:19, 1 November 2017

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Description

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Find out why plasmid copy number and multiple plasmid systems are so important, what is SynORI, how does it work, and how can you apply it.

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Description

What is SynORI?

SynORI stands for synthetic origin of replication. It is a framework designed to make working with single and multi-plasmid systems precise, easy and on top of that - more functional.

In a standardized way, this system enables scientist to build a plasmid system by:

  1. Selecting the number of plasmid groups
  2. Picking the type of copy number control (specific to one group or regulating all of them at once)
  3. Choosing the copy number of each group.

The framework also comes with the possibility of adding a selection system that reduces the usage of antibiotics (only 1 antibiotic for up to 5 plasmids!) and an active partitioning systems to make sure low copy plasmid groups are not lost during division.

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Applications

Everyday lab work

Easy to assemble and control multi-plasmid system. With our framework there is no need to limit your research to a particular plasmid copy number just because there are no right replicons to choose from. With SynORI you can easily create a vector with a desired copy number that suits your needs.

Biological computing

The ability to choose from a wide range of copy number options and their control type will make synthetic biology engineering much more flexible and predictable. Regulation of plasmid copy number is equivalent to adding a global parameter into a computer system. This enables the coordination of multiple gene group expression.

Smart assembly of large protein complexes

Co-expression of multi-subunit complexes using different replicons brings incoherency to an already chaotic cell system. This can be avoided by using SynORI, as every plasmid group uses the same type of control, yet can act in a group-specific way.

Metabolic engineering

A big challenge for heterologous expression of multiple gene pathways is to accurately adjust the levels of each enzyme to achieve optimal production efficiency. Precise promoter tuning in transcriptional control and synthetic ribosome binding sites in translational control are already widely used to maintain expression levels. In addition to current approaches, our framework offers multiple gene control at the same time and on top of that, inducible regulation can make the search for perfect conditions a lot easier.