Difference between revisions of "Team:Bordeaux/Description"
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<h1>Description</h1> | <h1>Description</h1> | ||
| − | <p>This year, IGEM bordeaux aims to study the alternative splicing of the unc-60 gene in <i>C. elegans</i>. This gene can be spliced differently, leading to the synthesis of different proteins. The alternative splicing of | + | <p>This year, IGEM bordeaux aims to study the alternative splicing of the unc-60 gene in <i>C. elegans</i>. This gene can be spliced differently, leading to the synthesis of different proteins. The alternative splicing of unc-60 depends on the precense of two proteins, SUP-12 and ASD-2. The proteins are expressed differently in different tissues of the organism. </p> |
| − | <p>In this project, we are trying to control the alternative splicing of unc-60. To do so, we want to control the expression of one of the protein important for the splicing : | + | <p>In this project, we are trying to control the alternative splicing of unc-60. To do so, we want to control the expression of one of the protein important for the splicing : SUP-12. Thus, we will be able to choose which isoform of the protein encoded by unc-60 will be produced. |
| − | On the one hand, we want to make a photo-inductible system using Neurospora carassa's White-Collar system. The splicing protein will be under control of the | + | On the one hand, we want to make a photo-inductible system using Neurospora carassa's White-Collar system. The splicing protein will be under control of the frq promoter, regulated by the White-Collar complex (expressed in the modified <i>C. elegans</i>). The White-Collar complex will fix the frq promoter in response to light stimulation, allowing the expression of SUP-12. |
| − | On the other hand, we aim to use another inductible system called the Q system (from Neurospora crassa). This system responds to the presence of quinic acid and could allow a more specific control of the splicing protein | + | On the other hand, we aim to use another inductible system called the Q system (from Neurospora crassa). This system responds to the presence of quinic acid and could allow a more specific control of the splicing protein SUP-12. The protein will be under the control of the QUAS promoter. this promoter can be recognise by the protein QF, initiating the transcription. But the repressive protein QS inhibits this activation of transcription. These two proteins, QF and QS, will be expressed in specific tissus. By adding quinic acid in the growth medium, we should be able to prevent the action of QS and thus allow the expression of SUP-12. |
| − | We also aim to create a microfluidic system to observe the results of these genetic modifications on C. elegans.</p> | + | We also aim to create a microfluidic system to observe the results of these genetic modifications on <i>C. elegans</i>.</p> |
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Revision as of 18:58, 3 July 2017
