|
|
Line 1: |
Line 1: |
− | {{Heidelberg/header2}}
| |
| | | |
− | {{Heidelberg/navbar}}
| |
− |
| |
− | {{#tag:html|
| |
− | {{Heidelberg/main|Title|Subtitle|https://static.igem.org/mediawiki/2017/8/88/T--Heidelberg--2017_modelling-graphical-abstract.svg|red|
| |
− | {{Heidelberg/abstract|https://static.igem.org/mediawiki/2017/8/88/T--Heidelberg--2017_modelling-graphical-abstract.svg|
| |
− | Successful in vivo directed evolution by PREDCEL and PACE requires the thorough consideration of experimental parameters, e.g. phage propagation times, culture dilution rates and inducer/inhibitor concentrations. We employed extensive ODE-based and stochastic modeling to identify the most sensitive parameters and adapt our experiments accordingly. First, we calibrated our models using phage propagation experiments from our wet lab complemented with literature data. Simulations showed that the phage titer is highly sensitive to culture dilution rates. We simulated batch times and transfer volumes for PREDCEL and corresponding flow rates for PACE to determine optimized conditions for gene pool selection while avoiding phage washout. We also estimated phage titer monitoring intervals for cost/labor efficient QC as well as inducer/inhibitor concentrations required to express the required mutagenic polymerases. Finally, provide a web-based, fully interactive modeling platform, not only extensively employed by our wet lab, but highly informs future iGEM teams building on our work.
| |
− | }}
| |
− | {{Heidelberg/templateus/Contentsection|
| |
− | {{Heidelberg/templateus/Tablebox|
| |
− | Table X: Title |
| |
− | {{#tag:html|
| |
− | <table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
| |
− | <thead> <tr>
| |
− | <th>TIme</th> <th>Date</th> <th>Operator</th> <th>Sample</th> <th>Action</th> <th>DONE ToDo:</th> <th>OD Turbidostat 2</th> <th>Stock ID</th> <th>TO DOs:</th> </tr>
| |
− | </thead><tbody> <tr>
| |
− | <td>12:00</td> <td>24/10/2017</td> <td>MP</td> <td>-</td> <td>Started filling the turbidostat (T2) with medium </td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>13:30</td> <td>24/10/2017</td> <td>MP</td> <td>-</td> <td>Inoculation of turbidostat 2 with 50 ml Stock culture </td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>14:00</td> <td>24/10/2017</td> <td>MP</td> <td>-</td> <td>Measured OD</td> <td></td> <td>"0</td> <td>18"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>15:00</td> <td>24/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>16:00</td> <td>24/10/2017</td> <td>MP</td> <td>-</td> <td>Measured OD</td> <td></td> <td>"0</td> <td>511"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>17:00</td> <td>24/10/2017</td> <td>MP</td> <td></td> <td>"Measured OD</td> <td> started filling the lagoon and the arabinose pump"</td> <td></td> <td>"0</td> <td>548"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>18:00</td> <td>24/10/2017</td> <td>MP</td> <td>1</td> <td>Inoculation with 1 ml phage supernatant</td> <td></td> <td>"0</td> <td>514"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>19:00</td> <td>24/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>20:00</td> <td>24/10/2017</td> <td>MS</td> <td>1</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 1"</td> <td></td> <td>"0</td> <td>637"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>21:00</td> <td>24/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>22:00</td> <td>24/10/2017</td> <td>MP</td> <td></td> <td>"Measured OD</td> <td> Flow rate for lagoon was to high from previous filling ( 7 ml/min in contrast to 1</td> <td>666 ml/min) - potential washout "</td> <td></td> <td>"0</td> <td>791"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>23:00</td> <td>24/10/2017</td> <td>MP</td> <td></td> <td>"Lagoon was infected with 0</td> <td>8 ml 10^8 PFU/ml phages to counteract the potential washout "</td> <td></td> <td>"0</td> <td>818"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>00:00</td> <td>25/10/2017</td> <td>MP</td> <td>2</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 2"</td> <td></td> <td>"0</td> <td>833"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>01:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>02:00</td> <td>25/10/2017</td> <td>MS</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>789"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>03:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>04:00</td> <td>25/10/2017</td> <td>MS</td> <td>3</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 3"</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>05:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td>"0</td> <td>792"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>06:00</td> <td>25/10/2017</td> <td>MS</td> <td></td> <td>Measured OD</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>07:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td>"0</td> <td>817"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>08:00</td> <td>25/10/2017</td> <td>TB</td> <td>4</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 4"</td> <td>Took samples</td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>09:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>10:00</td> <td>25/10/2017</td> <td>TB</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>816"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>11:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>12:00</td> <td>25/10/2017</td> <td>TB</td> <td></td> <td>Measured OD</td> <td>Done PCR</td> <td>"0</td> <td>81"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>13:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>14:00</td> <td>25/10/2017</td> <td>PP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>843"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>15:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td>inoculated growth for plaque assay</td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>16:00</td> <td>25/10/2017</td> <td>PP</td> <td>5</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 5"</td> <td></td> <td>"0</td> <td>833"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>17:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>18:00</td> <td>25/10/2017</td> <td>PP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>836"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>19:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>20:00</td> <td>25/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>862"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Plaque Assay</td> </tr>
| |
− | <tr>
| |
− | <td>21:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>22:00</td> <td>25/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>81"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>23:00</td> <td>25/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>00:00</td> <td>26/10/2017</td> <td>LP</td> <td>6</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 6"</td> <td>Done PCR</td> <td>"0</td> <td>809"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>01:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>02:00</td> <td>26/10/2017</td> <td>CG</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>845"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>03:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>04:00</td> <td>26/10/2017</td> <td>CG</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>864"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>05:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>06:00</td> <td>26/10/2017</td> <td>CG</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>836"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>07:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>08:00</td> <td>26/10/2017</td> <td>TB</td> <td>7</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 7"</td> <td></td> <td>"0</td> <td>831"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>09:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>10:00</td> <td>26/10/2017</td> <td>TB</td> <td></td> <td>Measured OD</td> <td> </td> <td>"0</td> <td>827"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>11:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>12:00</td> <td>26/10/2017</td> <td>TB</td> <td></td> <td>Measured OD</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>13:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>14:00</td> <td>26/10/2017</td> <td>TB</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>738"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>15:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>16:00</td> <td>26/10/2017</td> <td>TB</td> <td>8</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 8"</td> <td>"Took samples L1</td> <td> T1"</td> <td>"0</td> <td>652"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>17:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>18:00</td> <td>26/10/2017</td> <td></td> <td></td> <td>Measured OD</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>19:00</td> <td>26/10/2017</td> <td>MS</td> <td></td> <td></td> <td></td> <td>"0</td> <td>582"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>20:00</td> <td>26/10/2017</td> <td></td> <td></td> <td>Measured OD</td> <td>Plaque assay was performed successfully </td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Plaque Assay</td> </tr>
| |
− | <tr>
| |
− | <td>21:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>22:00</td> <td>26/10/2017</td> <td>MS</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>633"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>23:00</td> <td>26/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>00:00</td> <td>27/10/2017</td> <td>PP</td> <td>9</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 9"</td> <td></td> <td>"0</td> <td>511"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>01:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>02:00</td> <td>27/10/2017</td> <td>MS</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>692"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>03:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>04:00</td> <td>27/10/2017</td> <td>MS</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>937"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>05:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td>"0</td> <td>945"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>06:00</td> <td>27/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td></td> <td>"could not be measured</td> <td> as lagoon medium level was too high and therefore pump where set off for a short time "</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>07:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>08:00</td> <td>27/10/2017</td> <td>MS</td> <td>10</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 10"</td> <td>"Phage detection PCR with samples 8</td> <td>9 and 10 for Dickinson-Phage and PI-Phage"</td> <td>"0</td> <td>912"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>09:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>10:00</td> <td>27/10/2017</td> <td>PP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>903"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>11:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>12:00</td> <td>27/10/2017</td> <td>LP</td> <td></td> <td>Measured OD</td> <td>see above</td> <td>"0</td> <td>924"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>13:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>14:00</td> <td>27/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>942"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>15:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>16:00</td> <td>27/10/2017</td> <td>PP</td> <td>11</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 11"</td> <td>took samples</td> <td>"0</td> <td>942"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>17:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>18:00</td> <td>27/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>822"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>19:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>20:00</td> <td>27/10/2017</td> <td>MK</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>59"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Plaque Assay</td> </tr>
| |
− | <tr>
| |
− | <td>21:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>22:00</td> <td>27/10/2017</td> <td>CG</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>61"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>23:00</td> <td>27/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>00:00</td> <td>28/10/2017</td> <td>MP</td> <td>12</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 12"</td> <td>Phage detection PCR with sample 11</td> <td>"0</td> <td>664"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>01:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>02:00</td> <td>28/10/2017</td> <td>CG</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>809"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>03:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>04:00</td> <td>28/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td>PCR: negative - phage washout</td> <td>"0</td> <td>820"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>05:00</td> <td>28/10/2017</td> <td></td> <td></td> <td>"Flow rate was adjusted: 1 ml/min </td> <td> lagoon was infected by pooled samples 6</td> <td> 7 and 8 "</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>06:00</td> <td>28/10/2017</td> <td>MP</td> <td></td> <td>Measured OD</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>07:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>08:00</td> <td>28/10/2017</td> <td>MP</td> <td>13</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 13"</td> <td></td> <td>"0</td> <td>667"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>09:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>10:00</td> <td>28/10/2017</td> <td>PP</td> <td></td> <td>Measured OD</td> <td></td> <td>"0</td> <td>62"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>11:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>12:00</td> <td>28/10/2017</td> <td>PP</td> <td></td> <td>Measured OD </td> <td>Phage PCR was performed </td> <td>"0</td> <td>768"</td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td>TO DO: Phage-PCR</td> </tr>
| |
− | <tr>
| |
− | <td>13:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>14:00</td> <td>28/10/2017</td> <td>PP</td> <td></td> <td>Measured OD</td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>15:00</td> <td>28/10/2017</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td>199 (pMax69 aka AP-SplitT7 + GenIII+YFP + MP4)</td> <td></td> </tr>
| |
− | <tr>
| |
− | <td>16:00</td> <td>28/10/2017</td> <td>PP</td> <td>14</td> <td>"Measured OD</td> <td> Took samples L1</td> <td> T1 - 14"</td> <td>FINAL PROBES</td> <td></td> <td></td> <td></td> </tr>
| |
− | </tbody></table>
| |
− |
| |
− |
| |
− | }}|Subtitle}}
| |
− | {{Heidelberg/accpanel|#000000|
| |
− | {{#tag:html|
| |
− | {{Heidelberg/accord|1|content 1|1}}
| |
− | {{Heidelberg/accord|2|content 2|2}}
| |
− | {{Heidelberg/accord|3|content 3|3}}
| |
− | }}
| |
− | }}
| |
− | }}
| |
− | }}
| |
− | }}
| |
− | {{Heidelberg/footer}}
| |