Tochingyuet (Talk | contribs) |
Tochingyuet (Talk | contribs) |
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<p><center><img src="https://static.igem.org/mediawiki/2017/3/32/CUHK_leakage.png" width="25%" height="auto" class=" igem-logo"></center></p> | <p><center><img src="https://static.igem.org/mediawiki/2017/3/32/CUHK_leakage.png" width="25%" height="auto" class=" igem-logo"></center></p> | ||
− | + | <p style="font-family: roboto;font-size:115%;"> | |
To activate toehold switch, an amount of energy is needed to open the toehold switch hairpin. The Switch MFE reflects the difficulty for the toehold switch unwinding process. We assume that the more negative the Switch MFE, the harder for the unwinding to take place, and hence a lower leakage. | To activate toehold switch, an amount of energy is needed to open the toehold switch hairpin. The Switch MFE reflects the difficulty for the toehold switch unwinding process. We assume that the more negative the Switch MFE, the harder for the unwinding to take place, and hence a lower leakage. | ||
</p> | </p> | ||
<br> | <br> | ||
<u><b><p style="font-family: roboto;font-size:125%;">Assumption: ΔG<sub>RBS-Linker</sub> correlates with the duplex expression</p></b></u> | <u><b><p style="font-family: roboto;font-size:125%;">Assumption: ΔG<sub>RBS-Linker</sub> correlates with the duplex expression</p></b></u> | ||
+ | <p style="font-family: roboto;font-size:115%;"> | ||
● ΔG<sub>RBS-Linker</sub> is the Gibbs free energy of the RNA sequence starting from the RBS to the linker in the switch-trigger duplex.<br> | ● ΔG<sub>RBS-Linker</sub> is the Gibbs free energy of the RNA sequence starting from the RBS to the linker in the switch-trigger duplex.<br> | ||
● The duplex expression is the reporter expression of the switch-trigger dimer RNA. | ● The duplex expression is the reporter expression of the switch-trigger dimer RNA. | ||
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<u><b><p style="font-family: roboto;font-size:125%;">Assumption: ΔMFE correlates with the duplex expression</b></u></p> | <u><b><p style="font-family: roboto;font-size:125%;">Assumption: ΔMFE correlates with the duplex expression</b></u></p> | ||
+ | <p style="font-family: roboto;font-size:115%;"> | ||
● ΔMFE is defined as MFE of the switch-trigger duplex RNA minus (switch RNA MFE + trigger RNA MFE). | ● ΔMFE is defined as MFE of the switch-trigger duplex RNA minus (switch RNA MFE + trigger RNA MFE). | ||
<br> | <br> |
Revision as of 19:51, 1 November 2017