Difference between revisions of "Team:Shanghaitech/Experiment and Modeling"
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<p><b>Fig. 1 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL</p> | <p><b>Fig. 1 Orthogonality test of LasR-pLas-GFP </b> (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL</p> | ||
<center><img src='https://static.igem.org/mediawiki/2017/0/04/T--Shanghaitech--rparortho1fx.png' /><center> | <center><img src='https://static.igem.org/mediawiki/2017/0/04/T--Shanghaitech--rparortho1fx.png' /><center> | ||
| + | <p><b>Fig. 2 Orthogonality test of RpaR-pRpa-GFP </b> (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with Coumaroyl-HSL</p> | ||
Revision as of 22:49, 1 November 2017
Experiment and Modeling
Testing Fluorescent AHL Receivers
Orthogonality test
Applying multiple quorumsensing systems in single bacteria requires low crosstalk activities between AHLs and LuxR-like proteins. We've tested signal molecule orthogonality in several Fluorescent AHL Receivers we construct.
Fig. 1 Orthogonality test of LasR-pLas-GFP (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with 3OC12-HSL
Fig. 2 Orthogonality test of RpaR-pRpa-GFP (i):Fluorescent response to cognate and non-cognate AHLs (ii)Dose-Response curves for cognate and non-cognate AHLs (iii-vi)Fluorescent response to non-cognate AHLs in compared with Coumaroyl-HSL
