Difference between revisions of "Team:CSU Fort Collins/Parts"
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<h1>Parts</h1> | <h1>Parts</h1> | ||
| − | <p> | + | <p>We developed pHF10, a derivative of pLC71, the only known vector plasmid for <i>Thermococcus kodakarensis</i>, as a chassis. We used restriction enzymes and ligation techniques to insert the limonene synthase gene while using <i>E. coli</i> during the design phase. The iGEM stadard vectors will not replicate within <i>Thermococcus kodakarensis</i>, so we chose to use a plasmid that would replicate in our chassis to continue our project. To our knowledge, there has been no other iGEM teams to use <i>Thermococcus kodakarensis</i>, so there are no parts available for us to use. for us except with pSB1C3. However, we had difficulty removing the limonene synthase gene from pHF10. This may be due to how we inserted it into the plasmid originally, or a complication from switiching plasmids.</p> |
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Revision as of 22:59, 1 November 2017
Parts
We developed pHF10, a derivative of pLC71, the only known vector plasmid for Thermococcus kodakarensis, as a chassis. We used restriction enzymes and ligation techniques to insert the limonene synthase gene while using E. coli during the design phase. The iGEM stadard vectors will not replicate within Thermococcus kodakarensis, so we chose to use a plasmid that would replicate in our chassis to continue our project. To our knowledge, there has been no other iGEM teams to use Thermococcus kodakarensis, so there are no parts available for us to use. for us except with pSB1C3. However, we had difficulty removing the limonene synthase gene from pHF10. This may be due to how we inserted it into the plasmid originally, or a complication from switiching plasmids.
