EllaKestrel (Talk | contribs) |
|||
Line 102: | Line 102: | ||
Working hard, we managed to successfully clone the Esterase gene into E.coli. Both colony PCR and sequencing were positive for the CE 5 esterase clone. Lipase, however, was not successfully cloned and the sequences came up negative and truncated.</article> | Working hard, we managed to successfully clone the Esterase gene into E.coli. Both colony PCR and sequencing were positive for the CE 5 esterase clone. Lipase, however, was not successfully cloned and the sequences came up negative and truncated.</article> | ||
+ | <article> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/ee/BaltimoreBiocrewFigure1.png" style="width:960px; height: 540px;"><!--original size: 1,920 × 1,080 pixels--> | ||
+ | </article> | ||
<article> | <article> | ||
Due to the negative results with the lipase, we hypothesized that the lipase enzyme was toxic to the strain of E. coli we were using, causing it to reject the DNA. | Due to the negative results with the lipase, we hypothesized that the lipase enzyme was toxic to the strain of E. coli we were using, causing it to reject the DNA. |
Revision as of 01:12, 2 November 2017