Difference between revisions of "Team:DTU-Denmark/Model"
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<figcaption> Figure 3: Occurrences of enzymatic rates of the models for each snake venom. </figcaption> | <figcaption> Figure 3: Occurrences of enzymatic rates of the models for each snake venom. </figcaption> | ||
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| + | <h1 class="bottomborder" id="lacz">LacZ Device</h1> | ||
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| + | <h4>Purpose</h4> | ||
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| + | <p>If β-galactosidase is used as a reporter in our biosensor, we would have an output of our device that depends on two subsequent enzymatic reactions. We want to model how the enzymatic activity of the first reaction affects the kinetics of the secondary reaction, and find some optimal time for the first reaction to run, if we want the combined time of both enzymatic reactions to be as short as possible.</p><br /><br /> | ||
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| + | <h4>Background</h4> | ||
| + | </p>In investigating if our prototype would be a viable option for in-field detection we decided to meet with Andreas Lausten, Co-founder of VenomAB, a producer of breakthrough solutions to modern antivenom monovalent production . As per his recommendation our system would ideally have to give a rapid response. As of yet, our assays had shown response time above 45 minutes for certain venoms, and we should seek to reduce the response time. In doing this we would have to amplify the output of our device compared to that of just using chromoproteins such as AmilCP. </p><br /><br /> | ||
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| + | <h4>Central assumptions</h4> | ||
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Revision as of 02:47, 2 November 2017
Substrate Set
Our substrate screening experiment produced a large number of interesting substrates for our detection assay. We looked at the raw fluorescence measurements, but we also applied statistical analysis to identify the peptides that had significant differences between the three venoms. In addition, we modelled the enzymatic activity of the venoms based on our measurements.
You can find the plots of logarithmic fluorescence signal and time of all the substrates here
The relative signal of each substrate between the three snakes was also analyzed. You can find that part here
Lastly, we looked at the fluorescence output in terms of enzymatic activity. We fit a non-linear model in the reactions and calculated their rates. You can find the models for all the substrates here
LacZ Device
Purpose
If β-galactosidase is used as a reporter in our biosensor, we would have an output of our device that depends on two subsequent enzymatic reactions. We want to model how the enzymatic activity of the first reaction affects the kinetics of the secondary reaction, and find some optimal time for the first reaction to run, if we want the combined time of both enzymatic reactions to be as short as possible.
