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Revision as of 05:07, 17 July 2017
Interlab
OD600 Reference Point
LUDOX-HS40 | H2O | |
---|---|---|
Replicate 1 | 0.097 | 0.082 |
Replicate 2 | 0.092 | 0.08 |
Replicate 3 | 0.09 | 0.082 |
Replicate 4 | 0.091 | 0.082 |
Arith. Mean | 0.0925 | 0.0815 |
Corrected Abs600 | 0.011 | |
Reference OD600 | 0.0425 | |
OD600/Abs600 | 3.863636364 |
Plate Pattern
A1 | A2 | A3 | A4 | A5 | A6 | A7 | A8 | A9 |
B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | B9 |
C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 |
D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 |
E1 | E2 | E3 | E4 | E5 | E6 | E7 | E8 | E9 |
F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 |
G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | G9 |
H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 |
Fluorescence Standard Curves
uM Fluorescein
uM Fluorescein | 50.00 | 25 | 12.5 | 6.25 | 3.125 | 1.5625 | 0.78125 | 0.390625 | 0.1953125 | 0.09765625 | 0.048828125 | 0 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Replicate 1 | 35189 | 28288 | 20495 | 16652 | 12162 | 8151 | 8016 | 8072 | 8362 | 7632 | 5075 | 8434 |
Replicate 2 | 34588 | 27070 | 18993 | 18141 | 11546 | 10531 | 8528 | 8507 | 7813 | 7638 | 5168 | 8517 |
Replicate 3 | 34302 | 28580 | 20980 | 16855 | 11988 | 9524 | 9391 | 8676 | 8107 | 7997 | 5116 | 8691 |
Replicate 4 | 34386 | 27353 | 21253 | 16614 | 11953 | 10064 | 8787 | 8667 | 7569 | 7550 | 5128 | 8053 |
Arith. Mean | 34616.25 | 27822.75 | 20430.25 | 17065.5 | 11912.25 | 9567.5 | 8680.5 | 8480.5 | 7962.75 | 7704.25 | 5121.75 | 8423.75 |
Arith. Std.Dev. | 400.2535655 | 725.0707437 | 1008.13735 | 724.7631797 | 260.7123255 | 1030.082359 | 571.830103 | 283.1825089 | 345.2886474 | 199.2525617 | 38.28293789 | 269.3688611 |
uM Fluorescein/a.u.
uM Fluorescein/a.u. | 50.00 | 25 | 12.5 | 6.25 | 3.125 | 1.5625 | 0.78125 | 0.390625 | 0.1953125 | 0.09765625 | 0.048828125 |
---|---|---|---|---|---|---|---|---|---|---|---|
Mean um Fluorescein/a.u. | 0.001444408 | 0.000898545 | 0.000611838 | 0.000366236 | 0.000262335 | 0.000163313 | 9.00006E-05 | 4.60616E-05 | 2.45283E-05 | 1.26756E-05 | 9.53348E-06 |
Mean of med-high levels: | 0.000460453 |
Abs600 Raw Readings
Hour 0
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.109 | 0.104 | 0.111 | 0.106 | 0.096 | 0.1455 | 0.107 | 0.106 | 0.0865 |
C1, R2 | 0.114 | 0.1185 | 0.1065 | 0.107 | 0.097 | 0.148 | 0.109 | 0.108 | 0.088 |
C1, R3 | 0.109 | 0.108 | 0.108 | 0.108 | 0.094 | 0.1465 | 0.109 | 0.107 | 0.086 |
C1, R4 | 0.1085 | 0.106 | 0.105 | 0.1065 | 0.096 | 0.1485 | 0.11 | 0.1095 | 0.091 |
C2, R1 | 0.108 | 0.1035 | 0.1105 | 0.1055 | 0.1305 | 0.0985 | 0.11 | 0.1145 | 0.087 |
C2, R2 | 0.109 | 0.105 | 0.111 | 0.1085 | 0.133 | 0.095 | 0.3745 | 0.114 | 0.086 |
C2, R3 | 0.1105 | 0.105 | 0.1115 | 0.1075 | 0.132 | 0.1325 | 0.272 | 0.4605 | 0.097 |
C2, R4 | 0.1115 | 0.108 | 0.112 | 0.107 | 0.132 | 0.095 | 0.108 | 0.1155 | 0.643 |
Hour 2
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.247 | 0.221 | 0.1125 | 0.1955 | 0.1965 | 0.2175 | 0.206 | 0.238 | 0.084 |
C1, R2 | 0.2315 | 0.206 | 0.1125 | 0.222 | 0.1925 | 0.207 | 0.2125 | 0.224 | 0.086 |
C1, R3 | 0.227 | 0.2075 | 0.1115 | 0.2145 | 0.195 | 0.2195 | 0.2135 | 0.2135 | 0.085 |
C1, R4 | 0.227 | 0.1975 | 0.1135 | 0.215 | 0.1935 | 0.2155 | 0.193 | 0.212 | 0.084 |
C2, R1 | 0.2295 | 0.204 | 0.1105 | 0.191 | 0.3015 | 0.179 | 0.209 | 0.2775 | 0.085 |
C2, R2 | 0.2325 | 0.2065 | 0.111 | 0.201 | 0.312 | 0.1805 | 0.1965 | 0.2665 | 0.085 |
C2, R3 | 0.225 | 0.19 | 0.1085 | 0.1905 | 0.293 | 0.163 | 0.174 | 0.242 | 0.0835 |
C2, R4 | 0.227 | 0.1915 | 0.108 | 0.1995 | 0.2955 | 0.1595 | 0.1965 | 0.2435 | 0.086 |
Hour 4
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.5255 | 0.549 | 0.13 | 0.54 | 0.4745 | 0.6265 | 0.5025 | 0.665 | 0.085 |
Colony 1, Replicate 2 | 0.465 | 0.505 | 0.124 | 0.5185 | 0.4115 | 0.4235 | 0.4905 | 0.569 | 0.099 |
Colony 1, Replicate 3 | 0.5155 | 0.5 | 0.126 | 0.51 | 0.4425 | 0.4875 | 0.501 | 0.532 | 0.084 |
Colony 1, Replicate 4 | 0.5115 | 0.5075 | 0.125 | 0.5105 | 0.457 | 0.4195 | 0.523 | 0.535 | 0.083 |
Colony 2, Replicate 1 | 0.5095 | 0.5055 | 0.117 | 0.519 | 0.626 | 0.407 | 0.49 | 0.696 | 0.085 |
Colony 2, Replicate 2 | 0.4635 | 0.4935 | 0.11 | 0.5005 | 0.5975 | 0.454 | 0.4475 | 0.562 | 0.085 |
Colony 2, Replicate 3 | 0.5005 | 0.496 | 0.1145 | 0.432 | 0.6055 | 0.4525 | 0.506 | 0.5465 | 0.085 |
Colony 2, Replicate 4 | 0.506 | 0.5 | 0.114 | 0.488 | 0.583 | 0.429 | 0.562 | 0.5665 | 0.084 |
Hour 6
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.681 | 0.7495 | 0.198 | 0.7775 | 0.781 | 0.7615 | 0.729 | 0.7285 | 0.087 |
Colony 1, Replicate 2 | 0.6595 | 0.7035 | 0.168 | 0.734 | 0.6505 | 0.7015 | 0.7275 | 0.6735 | 0.088 |
Colony 1, Replicate 3 | 0.6635 | 0.7295 | 0.167 | 0.752 | 0.659 | 0.7205 | 0.419 | 0.7025 | 0.085 |
Colony 1, Replicate 4 | 0.655 | 0.724 | 0.162 | 0.708 | 0.727 | 0.6975 | 0.6985 | 0.684 | 0.085 |
Colony 2, Replicate 1 | 0.666 | 0.957 | 0.1645 | 0.6915 | 0.747 | 0.719 | 0.962 | 0.944 | 0.085 |
Colony 2, Replicate 2 | 0.6385 | 0.734 | 0.1295 | 0.6155 | 0.773 | 0.666 | 0.665 | 0.729 | 0.085 |
Colony 2, Replicate 3 | 0.6445 | 0.731 | 0.131 | 0.6445 | 0.754 | 0.611 | 0.6975 | 0.7275 | 0.085 |
Colony 2, Replicate 4 | 0.626 | 0.668 | 0.128 | 0.67 | 0.759 | 0.6395 | 0.6885 | 0.695 | 0.085 |
Fluorescence Raw Readings
Hour 0
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 32 | 79 | 255 | 113 | 30 | 145 | 28 | 26 | 25 |
Colony 1, Replicate 2 | 36 | 81 | 225 | 130 | 25 | 129 | 35 | 37 | 23 |
Colony 1, Replicate 3 | 32 | 82 | 278 | 122 | 29 | 133 | 29 | 29 | 29 |
Colony 1, Replicate 4 | 27 | 88 | 258 | 112 | 31 | 134 | 33 | 27 | 20 |
Colony 2, Replicate 1 | 30 | 48 | 255 | 114 | 36 | 30 | 27 | 25 | 22 |
Colony 2, Replicate 2 | 36 | 66 | 253 | 115 | 29 | 41 | 40 | 27 | 17 |
Colony 2, Replicate 3 | 36 | 71 | 255 | 110 | 30 | 35 | 29 | 33 | 34 |
Colony 2, Replicate 4 | 34 | 62 | 249 | 118 | 36 | 28 | 40 | 41 | 33 |
Hour 2
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 42 | 385 | 338 | 391 | 31 | 469 | 152 | 38 | 15 |
Colony 1, Replicate 2 | 37 | 345 | 322 | 459 | 28 | 403 | 146 | 35 | 12 |
Colony 1, Replicate 3 | 25 | 350 | 323 | 406 | 25 | 474 | 126 | 37 | 25 |
Colony 1, Replicate 4 | 36 | 327 | 352 | 417 | 38 | 449 | 118 | 33 | 17 |
Colony 2, Replicate 1 | 32 | 304 | 315 | 352 | 37 | 155 | 130 | 25 | 37 |
Colony 2, Replicate 2 | 26 | 297 | 319 | 388 | 39 | 156 | 116 | 45 | 47 |
Colony 2, Replicate 3 | 32 | 273 | 270 | 370 | 48 | 125 | 92 | 37 | 24 |
Colony 2, Replicate 4 | 34 | 277 | 269 | 381 | 54 | 128 | 111 | 33 | 40 |
Hour 4
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 31 | 783 | 497 | 893 | 40 | 1245 | 188 | 36 | 42 |
Colony 1, Replicate 2 | 30 | 730 | 495 | 825 | 34 | 801 | 183 | 39 | 35 |
Colony 1, Replicate 3 | 61 | 719 | 494 | 792 | 46 | 931 | 178 | 43 | 39 |
Colony 1, Replicate 4 | 35 | 746 | 523 | 832 | 39 | 829 | 185 | 42 | 25 |
Colony 2, Replicate 1 | 36 | 657 | 392 | 853 | 44 | 286 | 182 | 54 | 12 |
Colony 2, Replicate 2 | 27 | 649 | 324 | 821 | 59 | 342 | 176 | 46 | 25 |
Colony 2, Replicate 3 | 39 | 645 | 365 | 701 | 58 | 350 | 208 | 52 | 28 |
Colony 2, Replicate 4 | 38 | 666 | 363 | 832 | 52 | 356 | 241 | 47 | 26 |
Hour 6
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 27 | 1181 | 873 | 1539 | 46 | 1396 | 280 | 59 | 47 |
Colony 1, Replicate 2 | 43 | 1108 | 635 | 1463 | 51 | 1281 | 264 | 45 | 25 |
Colony 1, Replicate 3 | 26 | 1115 | 625 | 1454 | 65 | 1287 | 151 | 25 | 24 |
Colony 1, Replicate 4 | 52 | 1141 | 614 | 1422 | 72 | 1252 | 262 | 28 | 30 |
Colony 2, Replicate 1 | 35 | 1360 | 651 | 1352 | 55 | 632 | 316 | 41 | 24 |
Colony 2, Replicate 2 | 44 | 1091 | 381 | 1176 | 57 | 581 | 233 | 33 | 22 |
Colony 2, Replicate 3 | 40 | 1065 | 373 | 1299 | 50 | 546 | 248 | 31 | 39 |
Colony 2, Replicate 4 | 33 | 1034 | 376 | 1363 | 49 | 583 | 252 | 47 | 14 |