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<div class="col-md-6 col-xs-12 text-center">
<h3>Plate Pattern</h3>
<h3>Plate Pattern</h3>
+ <h5> This diagram depicts how our samples were organized on our plate.
{{Data/csv/Plate_Pattern}}
{{Data/csv/Plate_Pattern}}
</div>
</div>
Revision as of 16:10, 19 July 2017
Interlab
iGEM's interlab study aims to establish a baseline and robust protocol for fluorescent measurements. Teams that participate in the interlab study will follow a standardized procedure that guides them through the transformation, inoculation and measurement processes. Below is the data that we collected for the Interlab Study:
OD600 Reference Point
LUDOX-HS40
H2O
Replicate 1
0.097
0.082
Replicate 2
0.092
0.08
Replicate 3
0.09
0.082
Replicate 4
0.091
0.082
Arith. Mean
0.0925
0.0815
Corrected Abs600
0.011
Reference OD600
0.0425
OD600/Abs600
3.863636364
Plate Pattern
This diagram depicts how our samples were organized on our plate.
A1
A2
A3
A4
A5
A6
A7
A8
A9
B1
B2
B3
B4
B5
B6
B7
B8
B9
C1
C2
C3
C4
C5
C6
C7
C8
C9
D1
D2
D3
D4
D5
D6
D7
D8
D9
E1
E2
E3
E4
E5
E6
E7
E8
E9
F1
F2
F3
F4
F5
F6
F7
F8
F9
G1
G2
G3
G4
G5
G6
G7
G8
G9
H1
H2
H3
H4
H5
H6
H7
H8
H9
Fluorescence Standard Curves
uM Fluorescein
uM Fluorescein
50.00
25
12.5
6.25
3.125
1.5625
0.78125
0.390625
0.1953125
0.09765625
0.048828125
0
Replicate 1
35189
28288
20495
16652
12162
8151
8016
8072
8362
7632
5075
8434
Replicate 2
34588
27070
18993
18141
11546
10531
8528
8507
7813
7638
5168
8517
Replicate 3
34302
28580
20980
16855
11988
9524
9391
8676
8107
7997
5116
8691
Replicate 4
34386
27353
21253
16614
11953
10064
8787
8667
7569
7550
5128
8053
Arith. Mean
34616.25
27822.75
20430.25
17065.5
11912.25
9567.5
8680.5
8480.5
7962.75
7704.25
5121.75
8423.75
Arith. Std.Dev.
400.2535655
725.0707437
1008.13735
724.7631797
260.7123255
1030.082359
571.830103
283.1825089
345.2886474
199.2525617
38.28293789
269.3688611
uM Fluorescein/a.u.
uM Fluorescein/a.u.
50.00
25
12.5
6.25
3.125
1.5625
0.78125
0.390625
0.1953125
0.09765625
0.048828125
Mean um Fluorescein/a.u.
0.001444408
0.000898545
0.000611838
0.000366236
0.000262335
0.000163313
9.00006E-05
4.60616E-05
2.45283E-05
1.26756E-05
9.53348E-06
Mean of med-high levels:
0.000460453
Abs600 Raw Readings
Hour 0
Hour 0:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.109
0.104
0.111
0.106
0.096
0.1455
0.107
0.106
0.0865
C1, R2
0.114
0.1185
0.1065
0.107
0.097
0.148
0.109
0.108
0.088
C1, R3
0.109
0.108
0.108
0.108
0.094
0.1465
0.109
0.107
0.086
C1, R4
0.1085
0.106
0.105
0.1065
0.096
0.1485
0.11
0.1095
0.091
C2, R1
0.108
0.1035
0.1105
0.1055
0.1305
0.0985
0.11
0.1145
0.087
C2, R2
0.109
0.105
0.111
0.1085
0.133
0.095
0.3745
0.114
0.086
C2, R3
0.1105
0.105
0.1115
0.1075
0.132
0.1325
0.272
0.4605
0.097
C2, R4
0.1115
0.108
0.112
0.107
0.132
0.095
0.108
0.1155
0.643
Hour 2
Hour 2:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.247
0.221
0.1125
0.1955
0.1965
0.2175
0.206
0.238
0.084
C1, R2
0.2315
0.206
0.1125
0.222
0.1925
0.207
0.2125
0.224
0.086
C1, R3
0.227
0.2075
0.1115
0.2145
0.195
0.2195
0.2135
0.2135
0.085
C1, R4
0.227
0.1975
0.1135
0.215
0.1935
0.2155
0.193
0.212
0.084
C2, R1
0.2295
0.204
0.1105
0.191
0.3015
0.179
0.209
0.2775
0.085
C2, R2
0.2325
0.2065
0.111
0.201
0.312
0.1805
0.1965
0.2665
0.085
C2, R3
0.225
0.19
0.1085
0.1905
0.293
0.163
0.174
0.242
0.0835
C2, R4
0.227
0.1915
0.108
0.1995
0.2955
0.1595
0.1965
0.2435
0.086
Hour 4
Hour 4:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
0.5255
0.549
0.13
0.54
0.4745
0.6265
0.5025
0.665
0.085
Colony 1, Replicate 2
0.465
0.505
0.124
0.5185
0.4115
0.4235
0.4905
0.569
0.099
Colony 1, Replicate 3
0.5155
0.5
0.126
0.51
0.4425
0.4875
0.501
0.532
0.084
Colony 1, Replicate 4
0.5115
0.5075
0.125
0.5105
0.457
0.4195
0.523
0.535
0.083
Colony 2, Replicate 1
0.5095
0.5055
0.117
0.519
0.626
0.407
0.49
0.696
0.085
Colony 2, Replicate 2
0.4635
0.4935
0.11
0.5005
0.5975
0.454
0.4475
0.562
0.085
Colony 2, Replicate 3
0.5005
0.496
0.1145
0.432
0.6055
0.4525
0.506
0.5465
0.085
Colony 2, Replicate 4
0.506
0.5
0.114
0.488
0.583
0.429
0.562
0.5665
0.084
Hour 6
Hour 6:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
0.681
0.7495
0.198
0.7775
0.781
0.7615
0.729
0.7285
0.087
Colony 1, Replicate 2
0.6595
0.7035
0.168
0.734
0.6505
0.7015
0.7275
0.6735
0.088
Colony 1, Replicate 3
0.6635
0.7295
0.167
0.752
0.659
0.7205
0.419
0.7025
0.085
Colony 1, Replicate 4
0.655
0.724
0.162
0.708
0.727
0.6975
0.6985
0.684
0.085
Colony 2, Replicate 1
0.666
0.957
0.1645
0.6915
0.747
0.719
0.962
0.944
0.085
Colony 2, Replicate 2
0.6385
0.734
0.1295
0.6155
0.773
0.666
0.665
0.729
0.085
Colony 2, Replicate 3
0.6445
0.731
0.131
0.6445
0.754
0.611
0.6975
0.7275
0.085
Colony 2, Replicate 4
0.626
0.668
0.128
0.67
0.759
0.6395
0.6885
0.695
0.085
Fluorescence Raw Readings
Hour 0
Hour 0:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
32
79
255
113
30
145
28
26
25
Colony 1, Replicate 2
36
81
225
130
25
129
35
37
23
Colony 1, Replicate 3
32
82
278
122
29
133
29
29
29
Colony 1, Replicate 4
27
88
258
112
31
134
33
27
20
Colony 2, Replicate 1
30
48
255
114
36
30
27
25
22
Colony 2, Replicate 2
36
66
253
115
29
41
40
27
17
Colony 2, Replicate 3
36
71
255
110
30
35
29
33
34
Colony 2, Replicate 4
34
62
249
118
36
28
40
41
33
Hour 2
Hour 2:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
42
385
338
391
31
469
152
38
15
Colony 1, Replicate 2
37
345
322
459
28
403
146
35
12
Colony 1, Replicate 3
25
350
323
406
25
474
126
37
25
Colony 1, Replicate 4
36
327
352
417
38
449
118
33
17
Colony 2, Replicate 1
32
304
315
352
37
155
130
25
37
Colony 2, Replicate 2
26
297
319
388
39
156
116
45
47
Colony 2, Replicate 3
32
273
270
370
48
125
92
37
24
Colony 2, Replicate 4
34
277
269
381
54
128
111
33
40
Hour 4
Hour 4:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
31
783
497
893
40
1245
188
36
42
Colony 1, Replicate 2
30
730
495
825
34
801
183
39
35
Colony 1, Replicate 3
61
719
494
792
46
931
178
43
39
Colony 1, Replicate 4
35
746
523
832
39
829
185
42
25
Colony 2, Replicate 1
36
657
392
853
44
286
182
54
12
Colony 2, Replicate 2
27
649
324
821
59
342
176
46
25
Colony 2, Replicate 3
39
645
365
701
58
350
208
52
28
Colony 2, Replicate 4
38
666
363
832
52
356
241
47
26
Hour 6
Hour 6:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
27
1181
873
1539
46
1396
280
59
47
Colony 1, Replicate 2
43
1108
635
1463
51
1281
264
45
25
Colony 1, Replicate 3
26
1115
625
1454
65
1287
151
25
24
Colony 1, Replicate 4
52
1141
614
1422
72
1252
262
28
30
Colony 2, Replicate 1
35
1360
651
1352
55
632
316
41
24
Colony 2, Replicate 2
44
1091
381
1176
57
581
233
33
22
Colony 2, Replicate 3
40
1065
373
1299
50
546
248
31
39
Colony 2, Replicate 4
33
1034
376
1363
49
583
252
47
14