Difference between revisions of "Team:Newcastle/Notebook/LabBookV1/June"

 
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     <h4 class="categoryheading31"><article id="June-28">June 28th</article></h4>
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     <H4 class="categoryheading31"><article id="June-28">June 28th</article></H4>
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     <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;">
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     <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;">      
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                 <li class="list">Description</li>
+
                 <li class="list"><b>Jess + Marcia </b></li>
−
                <li class="list">Details of this thing follow</li>
+
 
                 <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;">
 
                 <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;">
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                     <li class="list">Details<sup>if you want a superscript</sup><sub>if you want a subscript</sub>
+
                     <li class="list">
 +
Overnight cultures made
 +
5 ml LB
 +
<br>
 +
<br>
 +
<u>Ampicillin</u>-100µg/ml<br>
 +
Stock-20 mg/ml<br>
 +
<br>
 +
C1V1=C2C2<br>
 +
V1x20 000=100x5
 +
V1=500/20 000
 +
V1=0.025ml=25µl<br>
 +
<br>
 +
 +
<u>Cam</u> - 12.5 µg/ml <br>
 +
Stock - 2.5 mg/ml <br>
 +
<br>
 +
V1 x 2500 = 2500 12,5 x 5 <br>
 +
V1 = 62.5/2 500 <br>
 +
V1 = 0.025 ml = 25 µl<br>
 
                 </ul>
 
                 </ul>
 
       </ul>
 
       </ul>
 
   </div>
 
   </div>
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<div class="lefttop">
 
<div class="lefttop">
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     <h4 class="categoryheading31"><article id="June-29">June 29th</article></h4>
+
     <H4 class="categoryheading31"><article id="June-29">June 29th</article></H4>
 
     <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;">
 
     <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;">
 
                 <li class="list">Description</li>
 
                 <li class="list">Description</li>
−
                 <li class="list">Details of this thing follow</li>
+
                 <li class="list"><b>Lais + Marcia + Ansh </b></li>
 
                 <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;">
 
                 <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;">
−
                     <li class="list">Details<sup>if you want a superscript</sup><sub>if you want a subscript</sub>
+
                     <li class="list">  
 +
Miniprep carried out according to Qaigen miniprep kit protocol <br>
 +
<br>
 +
              Conc (ng/µl)  Trial 2 Conc (ng/µl) <br>
 +
SGFP          248.8 <br>
 +
J5100          179.1 <br>
 +
K516030    78.7          113.2 <br>
 +
K1758500  139.5 <br>
 +
B0534        80.7            90.4 <br>
 +
J33201        128.9 <br>
 +
J04500        172.2 <br>
 +
K143012    91.1          105.4 <br>
 +
I70468        143.5 <br>
 +
B0010        199.9 <br>
 +
C0171        99.4          106.4<br>
 
                 </ul>
 
                 </ul>
 
       </ul>
 
       </ul>
 
   </div>
 
   </div>
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+
 
−
<div class="lefttop">
+
  <div class="lefttop">
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     <h4 class="categoryheading31"><article id="June-30">June 30th</article></h4>
+
     <H4 class="categoryheading31"><article id="June-30">June 30th</article></H4>
 
     <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;">
 
     <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;">
−
                 <li class="list">Description</li>
+
                 <li class="list"><b>Declan + Lais </b></li>
−
                <li class="list">Details of this thing follow</li>
+
 
                 <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;">
 
                 <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;">
−
                     <li class="list">Details<sup>if you want a superscript</sup><sub>if you want a subscript</sub>
+
                     <li class="list">
 +
 +
Digest prep - <br>
 +
16 µl sample <br>
 +
2 µl buffer <br>
 +
1 µl PST1 <br>
 +
1 µl EcoRI <br>
 +
<br>
 +
Digest at 37C for 1 hour <br>
 +
<br>
 +
Agarose gel prepped - 0.8% gel = 0.8 g in 1000 ml of TA bugger (1M) <br>
 +
<br>
 +
Dye calculation - 20 µl in PCR tubes, dye needs to be 6x <br>
 +
<br>
 +
Digested - so 20/6 = 3.666 µl in each <br>
 +
Undigested - 6/6 = 1 µl <br>
 +
<br>
 +
<br>
 +
Gel plan -  <br>
 +
Lanes <br>
 +
1 - 100 bp ladder <br>
 +
2 - I7468 <br>
 +
3 - B0534 <br>
 +
4 - K516030 <br>
 +
5 - C0171 <br>
 +
6 - K143012 <br>
 +
7 - J5100 <br>
 +
8 - J04500 <br>
 +
9 - B0010 <br>
 +
10 - K1758500<br>
 +
11 - J33001 <br>
 +
12 - 1 kb ladder<br>
 
                 </ul>
 
                 </ul>
 
       </ul>
 
       </ul>
 
   </div>
 
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     * Lab Book Monthly Summary End
 
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Latest revision as of 22:24, 1 August 2017

Our wiki is currently under construction, please bear with us while we make it functional


Untitled Page

June 1st

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    • Detailsif you want a superscriptif you want a subscript

June 2nd

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June 3rd

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June 4th

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June 5th

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June 6th

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June 7th

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June 8th

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June 9th

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June 10th

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June 11th

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June 12th

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June 13th

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June 14th

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June 15th

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June 16th

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June 17th

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June 18th

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June 19th

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June 20th

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June 21st

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June 22nd

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June 23rd

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June 24th

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June 25th

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June 26th

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June 27th

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June 28th

  • Jess + Marcia
    • Overnight cultures made 5 ml LB

      Ampicillin-100µg/ml
      Stock-20 mg/ml

      C1V1=C2C2
      V1x20 000=100x5 V1=500/20 000 V1=0.025ml=25µl

      Cam - 12.5 µg/ml
      Stock - 2.5 mg/ml

      V1 x 2500 = 2500 12,5 x 5
      V1 = 62.5/2 500
      V1 = 0.025 ml = 25 µl

June 29th

  • Description
  • Lais + Marcia + Ansh
    • Miniprep carried out according to Qaigen miniprep kit protocol

      Conc (ng/µl) Trial 2 Conc (ng/µl)
      SGFP 248.8
      J5100 179.1
      K516030 78.7 113.2
      K1758500 139.5
      B0534 80.7 90.4
      J33201 128.9
      J04500 172.2
      K143012 91.1 105.4
      I70468 143.5
      B0010 199.9
      C0171 99.4 106.4

June 30th

  • Declan + Lais
    • Digest prep -
      16 µl sample
      2 µl buffer
      1 µl PST1
      1 µl EcoRI

      Digest at 37C for 1 hour

      Agarose gel prepped - 0.8% gel = 0.8 g in 1000 ml of TA bugger (1M)

      Dye calculation - 20 µl in PCR tubes, dye needs to be 6x

      Digested - so 20/6 = 3.666 µl in each
      Undigested - 6/6 = 1 µl


      Gel plan -
      Lanes
      1 - 100 bp ladder
      2 - I7468
      3 - B0534
      4 - K516030
      5 - C0171
      6 - K143012
      7 - J5100
      8 - J04500
      9 - B0010
      10 - K1758500
      11 - J33001
      12 - 1 kb ladder

June

Mo Tu We Th Fr Sa Su
29 30 31 1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 1 2

The Important Things We Did In June

Summary