Difference between revisions of "Team:Newcastle/Notebook/LabBookV1/July"
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<h4 class="categoryheading31"><article id="July-6">July 6th</article></h4> | <h4 class="categoryheading31"><article id="July-6">July 6th</article></h4> | ||
<ul style="list-style-image:none;list-style-type: disc;padding-left:40px;"> | <ul style="list-style-image:none;list-style-type: disc;padding-left:40px;"> | ||
| − | <li class="list"> | + | <li class="list"><b>Marcia, Sophie + Ansh </b></li> |
| − | + | ||
<ul style="list-style-image:none;list-style-type: disc;padding-left:12px;"> | <ul style="list-style-image:none;list-style-type: disc;padding-left:12px;"> | ||
| − | <li class="list"> | + | <li class="list"> |
| + | <u>Digestion </u><br> | ||
| + | MP17 <br> | ||
| + | • 2 µl buffer (cutsmart) <br> | ||
| + | • 3.8 µl DNA <br> | ||
| + | • 1 µl Pst1 <br> | ||
| + | • 1 µl Spe1 <br> | ||
| + | • 12.2 µl water<br> | ||
| + | <br> | ||
| + | MP21 <br> | ||
| + | • 2 µl buffer (cutsmart) <br> | ||
| + | • 8.5 µl DNA <br> | ||
| + | • 1 µl Pst1 <br> | ||
| + | • 1 µl Xba1 <br> | ||
| + | • 7.5 µl water <br> | ||
| + | <br> | ||
| + | Incubate at 37C 1 hour, 64C 20 minutes, 4C rest<br> | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
Revision as of 22:57, 1 August 2017
Our wiki is currently under construction, please bear with us while we make it functional
July 1st
- Description
- Details of this thing follow
- Detailsif you want a superscriptif you want a subscript
July 2nd
- Description
- Details of this thing follow
- Detailsif you want a superscriptif you want a subscript
July 3rd
- Marcia + Lais
-
Digest ran for K1632013 (MP17) and K137058 (MP18)
37C 1 hour, 64C 20 minutes, 4C rest
2 copies of each sample digested
17 + 18 - Arac/FimE
22 + 21 - FimS/GFP
For ligation, samples were quantifies on the nanodrop
MP17 - 264.2 ng/µl
MP18 - 306.8 ng/µl
MP22 - 143.9 ng/µl
MP21 - 117.5 ng/µl
Competent cell test kit Calculating cell efficiency
Ran gel + cut bands
MP17 - 0.193 g
MP18 - 0.205 g
MP21 - 0.202 g
MP22 - 0.292 g
Gel purified using the Qiagen purification kit
Competent cells testing kit using the iGEM protocol
Controls-
DH5α + H20 in LB
-
DH5α + H20 on cm
-
DH5α + GFP on cm
-
DH5α + mRFP on amp
-
DH5α + H20 in LB
July 4th
-
Fim Switch
- Ansh + Lais
-
Counting cell efficiency
---10 50 100
1 - 2 20 46
2 - 9 37 73
3 - 6 36 34
- Lais, Ansh, Marcia + Sophie
- Transformation
July 5th
- Description
- Details of this thing follow
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July 6th
- Marcia, Sophie + Ansh
-
Digestion
MP17
• 2 µl buffer (cutsmart)
• 3.8 µl DNA
• 1 µl Pst1
• 1 µl Spe1
• 12.2 µl water
MP21
• 2 µl buffer (cutsmart)
• 8.5 µl DNA
• 1 µl Pst1
• 1 µl Xba1
• 7.5 µl water
Incubate at 37C 1 hour, 64C 20 minutes, 4C rest
July 7th
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July 8th
- Description
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July 9th
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July 10th
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July 11th
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July 12th
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July 13th
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July 14th
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July 15th
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July 16th
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July 17th
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July 18th
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July 19th
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July 20th
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July 21st
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July 22nd
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July 23rd
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July 24th
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July 25th
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July 26th
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July 27th
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July 28th
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July 29th
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July 30th
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