Team:BOKU-Vienna/Protocol

Protocol

Protocols

Contents

• MATERIALS
  • LB medium
  • LB agar
• METHODS
  • Preparation of chemically competent E. coli
  • Transformation of chemically competent E. coli
  • Preparation of electro competent S. cerevisiae
  • Plasmid DNA Extraction
  • InterLab Study
  • Q5 PCR
  • oneTaq colony PCR
  • Golden Gate
  • OneTaq Colony PCR

Materials

LB medium

Name	Amount
Peptone	10 g
Yeast Extract	5 g
NaCl	5 g
RO-water	1000 mL

LB agar

Name	Amount
Peptone	10 g
Yeast Extract	5 g
NaCl	5 g
RO water	986 mL
Agar	14 g

Methods

Preparation of chemical competent E. coli