Team:SDU-Denmark/test

PowerLeaf - a bacterial solar battery

ENERGY STORED IN CELLULOSE • LIGHT-SENSING DORMANCY SYSTEM • OPTIMIZED NANOWIRES

Abstract


The PowerLeaf introduces a novel solution for long-term storage of solar energy, thus becoming an alternative to solar cells. This is accomplished without the use of environmentally harmful resources. The device is designed to resemble a plant leaf, which is meant to provide a nature-in-city ambience. This hypothetical implementation of the PowerLeaf in an urban environment, was developed through public engagement and collaboration.
The bacterial solar battery is composed of an energy storing unit (1), and an energy converting unit (2). The energy storing unit (1) is defined by a genetically engineered Escherichia Coli, which fixates carbon dioxide into the chemically stable polymer cellulose. A light sensing system activates dormancy during nighttime, to reduce energy lost by metabolism. The energy converting unit (2) uses genetically engineered Geobacter Sulfurreducens to consume the stored cellulose. Retrieved electrons are transferred by optimized nanowires to an anode resulting in an electrical current.

All on one page.
Just keep scrolling.

Presentation.

Welcome to our wiki! We are the IGEM team from the University of Southern Denmark. We have been waiting with great anticipation for the chance to introduce you to our project, to show you our iGEM experiences, and to tell you our story. It all began with a meeting between a group of strangers - each with their own disciplinary origin. Despite our differences, we had one thing in common. A shared interest in synthetic biology. We were soon herded off to a weekend in a cottage, far away from our regular lives. It was a place to bond, and to discuss project ideas. It immediately became apparent, that being an interdisciplinary team, was going to be our strength. Each member had unique qualities, that enabled them to efficiently tackle different aspects of the iGEM competition. So we made it our goal to take advantage of these qualities.
We came up with the idea to make a proof-of-concept project. Specifically, we wanted to use bacteria as a novel and greener solution for solar energy storage. This project was later dubbed the PowerLeaf – a bacterial solar battery. There is so much to tell you about our bacterial solar battery and its’ amazing features.
Our story is told here on the wiki, and since it’s a one-page wiki, just keep scrolling and we will gently guide you through our project.

Team


Welcome to the team page. Here you can get to know us on a more personal level. We are 12 students from 8 different majors. As a team, we have had the most amazing summer together. We had road trips, dinners, various activities and lots of fun together! Heck, we even celebrated Christmas in July. We shared all of this fun with our wonderful supervisors.
So go on and meet us, we don't bite. At least the most of us don't.


Emil Bøgh Hansen


Study: Biology
E-mail: ehans15@student.sdu.dk
Howdy! I’m the first Emil and the teams biologist! And I am a huge wolf enthusiast! I put my boots in the closet this summer in order to put on a proper lab coat doing iGEM. Besides my time in the lab I also looked into how GMOs can influence the environment.

Emil Søndergaard


Study: History
E-mail: emsoe09@student.sdu.dk
Ahoy thar! My name is Emil, and I want to be the next Indiana Jones. But before i can raid any tombs, i’ve decided to raid iGEM trophies. When i’m not cooking or travelling, I’m drawing on my background in history for communications and human practices.

Emil Vyff Jørgensen


Study: Physics
E-mail: ejoer15@student.sdu.dk
Mojn! I am another Emil and I might not be the model biochemist, so instead I am modelling biochemistry! My iGEM existence is a stochastic binary function between naps and extreme bursts of energy.

Ellen Gammelmark


Study: Biochemistry and Molecular Biology
E-mail: elgam15@student.sdu.dk
Why, hello there! My name is Ellen, and I spend most of my waking hours either in the lab with a pipette in my hand or just outside it with a computer on my lap. You know.. Learn iGEM, live iGEM, love iGEM

Felix Boel Pedersen


Study: Biochemistry and Molecular Biology
E-mail: feped15@student.sdu.dk
Aloha. My name is Felix and I bring joy to others by eating my daily rugbrød with leverpostej. 2 years and still going strong, magical. Speaking of magic, I’m the team’s wiki lizard. I also go in the lab from time to time when the others miss me too much.

Frederik Bartholdy Flensmark Neergaard


Study: Biochemistry and Molecular Biology
E-mail: frnee15@student.sdu.dk
Hey yo! I’m Frederik and I have worked day and night, mostly drinking beers in the night time but that should count as well. When not working in lab or on the pc I make fun with the other teammates and tell bad dad jokes, also I make crazy ideas come true like celebrating christmas in august.

Frederik Mark Højsager


Study: Medicine
E-mail: frhoe14@student.sdu.dk
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Jonas Borregaard Eriksen


Study: Pharmacy
E-mail: jerik15@student.sdu.dk
Yo!!! I’m Jonas and … During the past months I have been sacrificing the last remainings of my soul to the GMO gods, by fulfilling my duty as a lab-slave at the SDU-Denmark iGEM team.

Lene Vest Munk Thomsen


Study: Philosophy
E-mail: letho11@student.sdu.dk
Hey, is it solipsistic in here, or is it just me? When not wondering whether or not there is an external world, I’ve been busy working out how to implement our solar battery into our local community and what to gain from doing so. Oh, and imposing metaethics on my team members, but I Kant go into detail with this already.

Malte Skovsager Andersen


Study: Biochemistry and Molecular Biology
E-mail: malta14@student.sdu.dk
Ey what up pimps I’m Malte, I’ve mostly been working in the lab wrapped in the dankest of lab coats doing the most exciting of experiments. All in the name of why the hell not. In the lab the utmost highest level of patience is needed, especially when tasked with testing if biobricks function as intended. This has, as seen in the image, caused me to pull out most of my hair.

Sarah Hyllekvist Jørgensen


Study: Biochemistry and Molecular Biology
E-mail: sajo415@student.sdu.dk
My main attribution to our project has primary been running about in the lab, even though going into depth with theory is my favorite occupation. Luckily, there is a clear link between wet- and dry-lab. I am the smallest member of the SDU iGEM team, but I have definitely rise to the occasion.

Sofie Mozart Mortensen


Study: Biomedicine
E-mail: sofmo15@student.sdu.dk
Hi there! My name is Sofie, and I am the team mama! I am the one who makes sure, that everyone gets their fair share of cake. When I’m not in the kitchen busy making cakes for my teammates you can find me in the lab, where I’m working on enhancing our systems cellulose production.

Nothing can be done alone, so please scroll further to read about the contributors, who helped make this project a reality.

Attributions


Laboratory, Technical and General support


We would like to give special thanks to our supervisors:

  • Assistant professor Mikkel Girke Jørgensen, for his general support and advice on development of the project, the laboratory, fundraising and team synergy.
  • Ph.D. student and former iGEM participant Patrick Rosendahl Andreassen, for his guidance and technical assistance in the laboratory.
  • Ph.D student and former iGEM participant Thøger Jensen Krogh, for his help in developing the wiki, as well as his laboratory guidance.
  • Cand.phil student and former iGEM participant Tim Munk, for his focus on team dynamics and advice for our human practices.

Thanks to:

  • Academic assistant Tina Kronborg for guidance in the lab and for providing us with lab equipment.
  • Medical Laboratory Technician, Simon Rose, for giving us a course in security and lab safety.
  • Postdoc Oona Sneoyenbos-West provided us with Geobacter sulfurreducens PCA and knowledge, on growing this bacterial strain. As well as lending, us her laboratory for culturing of the Geobacter sulfurreducens PCA.
  • Business scout and PhD Ann Zahle Andersen presented tools to us for the development of innovative business ideas.
  • Stud.scient Kristian Severin Rasmussen helped us using the oCelleScope for testing.
  • Stud.scient and former iGEM participant Brian Baltzar for hosting a workshop about graphical representation in Adobe Illustrator.
  • Title Jonas Hartwig for helping with some JQuery functionality on the wiki.
  • Stud.scient Birka Jensen, for advice on how to build an iGEM wiki.
  • Stud.med Ida Charlotte Hvam for helpful discussions on development of the wiki.
  • Ph.D student and current iGEM advisor of the team from Bielefeld, Boas Pucker has provided us with BioBricks created by former iGEM teams from Bielefeld.
  • Dem fra Imperial?
  • Stud.polyt Oliver Klinggaard, for helpful discussions on the implementation of a pan-tilt system and for providing os with his project report on the subject.
  • DTU BioBuilders hosted their 5th Annual Biobrick Workshop.
  • The UNIK Copenhagen iGEM team hosted the Nordic Meetup.
  • The TU-Delft iGEM team for hosting the European Meetup.
  • The Danish Science Festival for having us at their annual event.
  • Mimo Antabi for adding our adverts to the university screens preceding the Danish Research Festival.
  • Allan Haurballe Madsen for the practical help associated with our appearance at the Danish Science Festival.
  • Lise Junker Nielsen for practical help associated with the Danish Science Festival and visit from the Academy for Talented Youth. As well as lending us iPads for laboratory use.
  • The Danish Science Festival for having us and to all the visitors attending our booth.
  • Dem der hjælper os med SDU-Meetup hvis nogen
  • Colombia til modelling
  • The high schools that we visited and presented our iGEM project at. These include Odense Technical gymnasium, Mulernes Legatskole and Academy for Talented Youth.
  • The UNF Camp for having us present our project to the students attending.
  • The elementary schools:
  • All former iGEM participants from SDU, attending our preliminary presentation and giving us feedback before the Giant Jamboree.
  • The following groups and associations, for helping us develop our human practices: (SP-Moulding, Borgernes Hus, Kommunens bygninger, Bolbro - områdefornyelse, Odense Byudvikling)
  • Matlab user Nezar for an easy implementation of the gillespie algorithm into matlab.
  • empty spot

Sponsors


Thanks to:

  • The Faculty of Science at University Southern Denmark for providing us with fundamental funds which have been required to make our project possible, and for providing lab benches and equipment.
  • The Faculty of Health Sciences at University of Southern Denmark for providing us with important funds to our project.
  • Integrated DNA Technologies for providing us with 20 kilobases of gBlock gene fragments.
  • SnapGene for providing our team with memberships to their software during the competition.
  • PentaBase for sponsoring us with 10.000 DKK worth of oligos and a further 10% discount.
  • Eurofins Genomics for providing us with an 80% discount on a Mix2Seq kit.

Litterature


Lots of nice articles and books.

Project Synergism


We have all been working together in every aspect of our project. Nevertheless, some people has had their focus on some areas more than others. The main groups are listed as follows;

  • The group focusing on fixation of CO2, production and secretion of cellulose and light-sensing dormancy consisted of Sarah Hyllekvist Jørgensen, Ellen Gammelmark, Sofie Mozart Mortensen and Emil Bøgh Hansen
  • The group focusing on the breakdown of cellulose to create an electrical current and optimization of nanowires consisted of Felix Boel Pedersen, Frederik Bartholdy Flensmark Neergaard, Jonas Borregaard Eriksen and Malte Skovsager Andersen.
  • The group focusing on implementation of the device into an urban environment, as well as our outreach consisted of Emil Søndergaard, Frederik Mark Højsager and Lene Vest Munk Thomsen.
  • The mathematical modelling of our project was single-handedly performed by Emil Vyff Jørgensen.
  • Coding and design of the wiki was performed by Felix Boel Pedersen and Frederik Mark Højsager.

Collaborations


Achievements


Introduction


Description


Human Outreach


Project & Results


Design of Components


Modelling


Experiments


Demonstration & Results


Parts & Procedures


Parts


Notebook


Protocols


Lab Safety


Practices


Bioethics


Integrated Practices


Events


Prospects


Perspectives


To Future iGEM Teams


Final Words