Team:Lethbridge HS/Contribution

Part Contributions

Basic Parts

3gt

The gene 3gt is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism Petunia hybrid. We have added this part to the registry as one of our new basic part submissions. Part BBa_idontknowwewillfindout

yadH

This Gene is an Escherichia coli gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. It is our second original basic part submission to the registry. It is uncharacterized and is a nter-membrane transport protein. Part BBa_wowzers.

Composite Parts

Anthocyanin Construct

This construct is being used to convert our initial molecule Eriodictyol into our final molecule Anthocyanin. The genes are:
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
f3h Part BBa_K1497009
dfr Part BBa_K1497010
ans Part BBa_K1497002
3gt Part BBa_waitingforsubmission
yadH Part BBa_alsowaiting
Terminator Part BBa_B0015.
These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The yadH has been shown to increase yields even though it is not directly involved in the pathway. The 3gt as well as yad H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart.

Zeaxanthin Construct

This construct is made up of the following genes:
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.
These parts all come together in our composite part BBa_Zeaxanthinconstruct. This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.

Indigoidine Construct

Our Indigoidine construct is made up of:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_waiting for actual part submission.
indC Part BBa_K1152013
Terminator Part BBa_B0015.
These two genes come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is one of our original basic part submissions. This construct is part BBa_idkyet

Melanin Construct

The parts used in our Melanin construct are as follows:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.
This construct allows us to make Melanin out of L-Tyrosine. it is Part BBa_waitandsee