Team:BostonU/Contribution

• 
• People
  Team Attributions
• Research
  Overview Experiments Results Modeling Notebook Protocols Safety
• Beyond the Bench
  Human Practices Integrated Human PracticesPublic EngagementCollaborations InterLab Contribution
• Achievements
  OverviewParts Judging Criteria

CONTRIBUTION TO THE INTERLAB STUDY

Fluorescence measurements are difficult to standardize across labs because different methods and units are used to measure fluorescence. In an effort to standardize fluorescence measurements from Green Fluorescent Protein (GFP), one of the most commonly used marker proteins, iGEM has implemented the InterLab Study in order to provide protocols for users to generate absolute units for measuring GFP. This is the fourth year iGEM has done the InterLab Study.

 

This year, iGEM is addressing this question: How close can the numbers be when fluorescence is measured all around the world? Parts from the iGEM InterLab Kit were transformed into competent E. coli DH5-alpha cells. Eight plasmids in total were transformed, including a Negative Control, a Positive Control, and six test devices.Their iGEM IDs are listed below. All measurements were made in a SpectraMax plate reader from a Costar black clear bottom 96-well plate.

Positive Control (BBa_I20270)

Negative Control (BBa_R0040)

Test Device 1 (BBa_J364000)

Test Device 2 (BBa_J364001)

Test Device 3 (BBa_J364002)

Test Device 4 (BBa_J364003)

Test Device 5 (BBa_J364004)

Test Device 6 (BBa_J364005)