Team:CMUQ/InterLab

BioSensor Lab

A1: T7 salt sensor

Introduction

The goal is amplify T7 salt sensor, digest it along with pSB1C3 plasmid with EcoRI and PstI

Materials

• Polymerase: Fusion Taq or Ampli tas (gold)
• Primers: PrefixF and SuffixR
• Nuclease free H2O
• 5xHF PCR Buffer
• gBlock DNA
• dNTPs
• PCR tubes

Procedure

PCR

1. Add the following reagents to a PCR tube:
2. Run the PCR machine

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