Team:AHUT China/Demonstrate

该项目的一系列实验具有多重意义,具体可分为:电影、数学模型及现实应用三个方面。 首先,针对灵感的起源:电影移动迷宫而言,我们的实践以技术的手段模拟了托马斯从迷宫中逃脱的过程,将艺术作品的思想由反映人性推广到技术应用的层面,故能够看成是电影世界的拓展。 其次,针对该数学问题:我们的实验与图论中常用的算法,如图搜索算法dijkstra等紧密关联。同时,我们使用合成生物学实验来解决一个经典的图论问题:求解可行路径,这在解决问题的方法中是一个很新颖的创新。 对于未来可能的现实应用:随着技术的进步,当生物计算机相关技术日趋成熟时,项目中的思想能够对导航等方面的应用有一定启发性。在2014、2016年我们团队的项目中,初始条件通常都是在给定的具体的参数下,力求找到一个最优解。但现实中的路况等情况错综复杂,需要系统在局部不断地进行相应的规划。而正是在此项目中,我们进行了初步的探索。 回到实验本身。在实验中,我们首先设立了引物作为端点,它们可类比地图中的起点与终点。之后,通过易错PCR的处理,使得DNA的序列内容发生了突变,以模拟多变的状况。但序列间需要相互连接才能实现有效的检测。故我们选择利用搭桥实验将先前的DNA进行配对,生成多条双链。进而,利用基因测序,即检测链首、链尾的基因,来判断得到的DNA是否为所需的。 考虑到前期需要考虑的因素多,相应的处理量可能较大。但通过几轮测试后,可进一步缩小影响因素,不断提升方法的效率。同时,虽然因为各方面的原因,我们的实验进行的不是特别顺利,但我们仍然得到了一些结果,并严格符合组委会的标准要求。 如下是我们的实验所得到的一些结果:
The series of experiments of this project have multiple meanings , which can be divided into three aspects : film , mathematical model and practical application .</br>
First of all , it is about the origin of inspiration——The Maze Runner. Our practice simulates Thomas ' s escape from the maze by means of technology , and expands the thoughts of art works from human nature reflection to technology application. In this case, it can be regarded as expansion of film domain.</br>
Secondly, it is about the mathematical problem. Our experiment is closely related to the algorithms used in graph theory , such as dijkstra algorithm. At the same time , we use synthetic biology experiments to solve a classical graph theory problem ——solving feasible paths , which is a novel innovation in solving problems.</br>
Thirdly, it is about the future application. With the development of technology , the thought in the project can be instructive to the application of navigation and other fields when the technology of bio-computer becomes more mature .In the 2014 and 2016 team project , the initial conditions are usually under given specific parameters .Actually, the situation of path condition in reality is complex , and it is necessary for the system to plan accordingly in a local area .And it is in this project that we conducted a preliminary exploration .</br>
In the experiment , we firstly set up primers as endpoints , which can be used to compare the starting point and end point in the map .Thereafter , the contents of the DNA sequence are mutated by the process of error-prone PCR, which is to simulate apolytropic conditions. The sequences need to be connected to each other in order to achieve effective detection, So we chose to pair the previous DNA using a bridge test to generate multiple double chains. Further , by using gene sequencing , i.e . detecting the gene of the chain head and the chain tail , it is judged whether or not the obtained DNA is desired .</br>
Considering that the factors need to be considered in the early stage is massive, the corresponding amount of processing may be large. However , after several rounds of testing, the influence factors can be further reduced, and the efficiency of the method can be continuously improved. At the same time, although our experiments were not particularly successful because of the various reasons, we still get some results that exactly meet the Committee' s standard requirements .</br>
Here are some of the results of our experiment :</br>