Team:Dalhousie/Improve
Improve
Part Improvement
Background This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax (BBa_K2160000). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose. Improvement We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose. http://parts.igem.org/Part:BBa_K2331000 LINKKKLearn more... hopefully have links to next pages here
