BBa_J04450, an RFP coding device, in a new iGEM chassis: Vibrio harveyi. Characterisation was made thanks to Petri dish plating and microscopic fluorescence observation.
BBa_K431009,the pGAP promoter, in the chassis Pichia pastoris, thanks to RTq-PCR experiments.
BBa_K1800001, an Alpha-Factor Secretion Signal, in the chassis Pichia pastoris thanks to toxicity assays.
BBa_K1072010, an Odr-10(diacetyl) receptor in the chassis P. pastoris, thanks to fluorescence essays in a microplate reader.
BBa_K1072023, the pFUS1 Promoter, in the chassis P. pastoris, thanks to fluorescence essays in a microplate reader.
Silver medal
#1: Out of 19 Parts, we obtained 6 new BioBricks compatible with the Registry. We successfully validated 3 of them:
BBa_K2278001, V. harveyi C8-CAI-1 (quorum sensing inducer) generator, with bioluminescence essays.
BBa_K2278021, coding for the D-NY15 Antimicrobial peptide with an Alpha-Factor Secretion Signal. Our favorite Part was validated thanks to sequencing, RTq-PCR experiments and toxicity assays on Petri dishes.
BBa_K2278011, pTet driven Diacetyl generator, validated thanks to NMR analysis.
#2: We are proud of all of the collaborations we made with teams around the world:
For the Purdue team, we determined the Lactobacillus spp resistance at -20°C in a sucrose and milk mix.
Thanks to the Vienna team, we obtained the part pGAP-RFP in P. pastoris.
We participated to the Postcard Project organized by the Gröningen team and in exchange received postcard from all around the world.
We participated to the European Meetup organized at TU Delft and to the French Meetup organized by the Pasteur Institute team. In each of these meetups, we obtained helpful feedbacks for our projects.
We skyped with 5 teams around the world (Greece, Singapore, Purdue, Boston, Gröningen). Talking with teams from different backgrounds, tackling their observation on our project and trying to understand theirs was very enlightening.
We filled out 17 surveys on various subjects.
#3: We carried out an ethical reflexion throughout all of our project, doing bibliographical research and meeting professionals and members of NGOs. Our major discussions and decisions are listed in our Human Practices Logbook.
Gold medal
#1: Our ethical reflexion went even further with the elaboration of 7 ethical matrices. Each matrix was a representation of our personal reflection on one key step of a water treatment product lifecycle. The outcomes of each matrix was carefully integrated in our project and could reshape part of it if needed.
#2: Modeling was a big part of our project, unavoidable considering the synthetic interactions of our three chassis. After building a standardized representation of all the genetic and molecular actors involved in communications, the challenge was to verify and simulate the functioning of our system through Ordinary Differential Equations. Then, sensitivity and robustness was analyzed thanks to Metabolic Control Analysis. Finally, a user-friendly interface was created for people non familiarized with ODEs.