Team:Lambert GA/Description


Description


In the face of conducting synthetic biology research, underfunded labs lack the monetary means of buying expensive laboratory equipment, such as a plate reader to measure fluorescence of cells or a centrifuge to pellet cells. Using engineering design principles, the 2017 Lambert iGEM team aimed to develop an imaging device called the Chrom-Q to cost-effectively standardize conditions for quantifying data; additionally, the team renovated the Paperfuge originally designed by the Prakash Lab to pellet cells at a low cost. Different chromoproteins - Tinsel Purple and Scrooge Orange - were induced at varying levels of IPTG, causing the cells to express different intensities; pictures of the cells were taken using a Chrom-Q and were measured with a self-constructed software app that analyzes the pigments using HSV color space. Additionally, the team observed that a common issue encountered by synthetic biologists is the overlap of protein expression in development of genetic circuits which negatively affects the quality of performance in a given cell. The 2017 Lambert iGEM Team worked to develop an inducible “switch” to further characterize ClpXP, a non-lysosomal proteolysis mechanism, with three Keio strains; the data showed a correlation between IPTG concentration and amount of protein degradation. Overall, Lambert iGEM desired to promote scientific research under any financial circumstance and to characterize non-lysosomal inducible protein degradation (CLiP’d).
















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