Team:Amazonas Brazil/Project
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Specifications
Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR
Scientific community had achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some basic methodological aspects related to this approach could be further improved to expand this science revolution.
Design
Check all the parts involved and our approaches with closer details!
Cas9 (BBa_K2457001): Standardized Cas9 device, regulated by the AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair pathways, it will lays the road to genomic editing in living cells.
