Team:NUS Singapore/Collaborations
Collaboration
IGEM Stockholm
1. Modelling
NUSgem assisted iGEM Stockholm with the modelling of two of their genetic circuits: Cumate and Tryptophan. Using their timing diagram and genetic circuit, we generated the functional models of these two genetic circuits. In addition to generating the functional models, we also performed sensitivity analysis and provided feedback and possible improvements that could be made to their designs.
To find out more about the iGEM Stockholm and our modelling contribution, click here
Stockholm Modelling Results!
2. Receiving E2 Colicin in Genome
iGEM Stockholm has worked on integrating E2 Toxin into E.Coli Genome. As part of the collaboration, they kindly shared with us their engineered chassis. We use this E2 Toxin integrated in the genome to formulate our Killswitch Chassi.
The E2 Colicin Integrated the Genome received by us! Thanks Stockholm!
US AFRL CarrolHS
1. Modelling
NUS-Gem provided the LabPats iGEM team with modelling support the for two of their genetics circuits: LabPats Plasmid 1; LabPats Plasmid 2. In addition to modelling their original circuits with RBS34, we also modelled their genetic circuits using RBS29-35. The results are shown below.
To find out more about our modelling collaboration with LabPats iGEM click here to download our report
To find out more about LabPats iGEM, click here
LabPats Modelled Genetic Circuits
2. Mentoring
NUSgem also provided LabPats iGEM with recommendations and ideas for human practice.
A skype session with LabPats!
3. Flat Stanely
NUSgem also participated in the Flat Stanley Collaboration initiative created by LabPats iGEM. The purpose of this initiative is connect other iGEM teams around the world together.
Connected!
IGEM-NTU
1. Characterisation of temperture sensitive system with GFP reporter
Our team had passed our transformed 10β cells containing the temperature sensitive system with GFP reporter (BBa_K2447014) to the NTU team for characterisation.
2. Double plasmid system containing dCas9 and gRNA/GFP reporter
This year, the NTU team is experimenting with the CRISPRi system, and to aid in the characterisation process of their proposed design of a truncated version of dCas9, our team had supplied them with this double plasmid system containing a GFP reporter.
3. Singapore IGEM meetup with NTU
iGEM Peshawar
1. Engineered Probiotics Survey
iGEM Peshawar kindly participated in our human practice survey providing valuable results that we folded into our design. To find out more about iGEM Peshawar, Click Here
2. 2. Discussion
NUSgem participated in meetings with iGEM Newcastle where we exchanged ideas and provided feedback regarding each other’s iGEM projects.
To find out more about iGEM Newcastle, Click here
To find out more about iGEM Toulouse, Click here
iGEM Newcastle
Engineered Probiotics Survey
NUS-GEM provided iGEM Stockholm with modelling support for their designs. NUS-GEM translated the genetic circuits and timing diagram and turned it into logic. Following the framework…
SynBio 7.0 with iGEM York, iGEM Cologne-Dusseldorf, iGEM-NTU, iGEM SVCE Chennai
NUS-GEM provided iGEM Stockholm with modelling support for their designs. NUS-GEM translated the genetic circuits and timing diagram and turned it into logic. Following the framework…
