Team:UST Beijing/Experiments
PanGu Cyclase Overview
Background
We used a composite part we constructed in 2016 iGEM competition (http://parts.igem.org/Part:BBa_K2072000:Design) for our following experiments. The plasmid pSB1C3/BBa_2072000 was transformed into E.coli BL21(DE3) cells and cultivated in 96-well microplates with following medium components: M9 salt mixture, chloramphenicol, glycerol and specific sugars, e.g. glucose, arabinose, or IPTG. PNPG was also included. The culture was shaken in 37 degrees C for up to 20 hours. The plate was analyzed with a microplate reader with wavelength at 405 and 620 nm. The data was collected in spreadsheet and analyzed using Matlab.
Strain used
Escherichia coli BL21(DE3)
Machines
CLC Sequence Viewer 6
Chimera 1.8
NCBI
Swiss Model
Methods
Protocols
Please click the link:
https://2017.igem.org/Competition/InterLab_Study>
