The vector plasmid with limonene synthase and empty vector control were successfully transformed into Thermococcus Kodakarensis.
The team correctly performed a GC analysis on the culture liquid, which unfortunately showed no limonene production. However, the headspace was not tested.
The team performed a western blot, which developed poorly and gave no results.
The organism also was difficult to grow, and many attempts to culture Kodakarensis ended with dead cultures.
