Team:Northwestern/Results
Results
Fractionation controls and protein expression
Figures 1-3 depict our fractionation controls and blot for the identification of saCas9 protein (expected size ~130 kB). The rightmost column in the blot, labelled "control" is a purified His-tagged protein and was used to ensure that the anti-His antibody could properly bind the protein in question. The fractionation results indicated that although Maltose-binding periplasmic protein (MBP) was exclusively present in the periplasmic fraction, showing strong bands of the expected size (42.5 kDa), GroEl was visible in both the cell's periplasm and cytoplasm (60 kDa) suggesting leakage from the cytoplasm to the periplasm. Although the positive control was successful, there was no evidence of saCas9 in the anti-His6 blot.
Cas9 functionality assay
OMV visualization
Vesicle delivery to Top10 cells - TEM visualization
Achievements
