Template:Heidelberg/best basic

BBa_K2398000

We present as best basic part the codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus that is able to convert silicon educts to organosilicon products.

This cytochrome c variant provides an easy to use tool that is accessible to everyone in the synthetic biology community and allows the user to harness the vast potential of organosilicons. This basic part exhibits a strong tendency to form silicon-carbon bonds and is a valuable addition to perform controlled organic chemistry in microorganisms. A triple mutant [Arnold et al., 2016] of this part has already been applied in the successful synthesis of organosilicons as a proof-of-concept. As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution (PACE) or in the phage-related discontinuous evolution (PREDCEL) approach to improve organosilicon synthesis by cytochrome engineering.

Figure 1:

Compounds used in the project of organosilicons and its respective products.

Figure 2:

Gas chromatogram for the reaction of educt (1) and (3) to the product (5). 11.7 minutes retention time indicates product formation. Unconverted educts converge 6.9 and 7.2 (7.4) minutes.

Figure 3:

Mass chromatogram shows the breakdown of the product (5) ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate. The product itself corresponds to a mass of 251 dalton.

Figure 4:

Gas chromatogram for the reaction of educt (2) and (3) to the product (4). 9.2 minutes retention time indicates product formation.

Figure 5:

Mass chromatogram shows the breakdown of the product (4) ethyl 2-(dimethyl(phenyl)silyl)propanoate. The product itself corresponds to a mass of 236 dalton.