iGEM's interlab study aims to establish a baseline and robust protocol for fluorescent measurements. Teams that participate in the interlab study will follow a standardized procedure that guides them through the transformation, inoculation and measurement processes. Below is the data that we collected for the Interlab Study:
OD600 Reference Point
LUDOX-HS40
H2O
Replicate 1
0.097
0.082
Replicate 2
0.092
0.08
Replicate 3
0.09
0.082
Replicate 4
0.091
0.082
Arith. Mean
0.0925
0.0815
Corrected Abs600
0.011
Reference OD600
0.0425
OD600/Abs600
3.863636364
Plate Pattern
This diagram depicts how our samples were organized on our plate:
A1
A2
A3
A4
A5
A6
A7
A8
A9
B1
B2
B3
B4
B5
B6
B7
B8
B9
C1
C2
C3
C4
C5
C6
C7
C8
C9
D1
D2
D3
D4
D5
D6
D7
D8
D9
E1
E2
E3
E4
E5
E6
E7
E8
E9
F1
F2
F3
F4
F5
F6
F7
F8
F9
G1
G2
G3
G4
G5
G6
G7
G8
G9
H1
H2
H3
H4
H5
H6
H7
H8
H9
Fluorescence Standard Curves
uM Fluorescein
uM Fluorescein
50.00
25
12.5
6.25
3.125
1.5625
0.78125
0.390625
0.1953125
0.09765625
0.048828125
0
Replicate 1
35189
28288
20495
16652
12162
8151
8016
8072
8362
7632
5075
8434
Replicate 2
34588
27070
18993
18141
11546
10531
8528
8507
7813
7638
5168
8517
Replicate 3
34302
28580
20980
16855
11988
9524
9391
8676
8107
7997
5116
8691
Replicate 4
34386
27353
21253
16614
11953
10064
8787
8667
7569
7550
5128
8053
Arith. Mean
34616.25
27822.75
20430.25
17065.5
11912.25
9567.5
8680.5
8480.5
7962.75
7704.25
5121.75
8423.75
Arith. Std.Dev.
400.2535655
725.0707437
1008.13735
724.7631797
260.7123255
1030.082359
571.830103
283.1825089
345.2886474
199.2525617
38.28293789
269.3688611
uM Fluorescein/a.u.
uM Fluorescein/a.u.
50.00
25
12.5
6.25
3.125
1.5625
0.78125
0.390625
0.1953125
0.09765625
0.048828125
Mean um Fluorescein/a.u.
0.001444408
0.000898545
0.000611838
0.000366236
0.000262335
0.000163313
9.00006E-05
4.60616E-05
2.45283E-05
1.26756E-05
9.53348E-06
Mean of med-high levels:
0.000460453
Abs600 Raw Readings
Hour 0
Hour 0:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.109
0.104
0.111
0.106
0.096
0.1455
0.107
0.106
0.0865
C1, R2
0.114
0.1185
0.1065
0.107
0.097
0.148
0.109
0.108
0.088
C1, R3
0.109
0.108
0.108
0.108
0.094
0.1465
0.109
0.107
0.086
C1, R4
0.1085
0.106
0.105
0.1065
0.096
0.1485
0.11
0.1095
0.091
C2, R1
0.108
0.1035
0.1105
0.1055
0.1305
0.0985
0.11
0.1145
0.087
C2, R2
0.109
0.105
0.111
0.1085
0.133
0.095
0.3745
0.114
0.086
C2, R3
0.1105
0.105
0.1115
0.1075
0.132
0.1325
0.272
0.4605
0.097
C2, R4
0.1115
0.108
0.112
0.107
0.132
0.095
0.108
0.1155
0.643
Hour 2
Hour 2:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.247
0.221
0.1125
0.1955
0.1965
0.2175
0.206
0.238
0.084
C1, R2
0.2315
0.206
0.1125
0.222
0.1925
0.207
0.2125
0.224
0.086
C1, R3
0.227
0.2075
0.1115
0.2145
0.195
0.2195
0.2135
0.2135
0.085
C1, R4
0.227
0.1975
0.1135
0.215
0.1935
0.2155
0.193
0.212
0.084
C2, R1
0.2295
0.204
0.1105
0.191
0.3015
0.179
0.209
0.2775
0.085
C2, R2
0.2325
0.2065
0.111
0.201
0.312
0.1805
0.1965
0.2665
0.085
C2, R3
0.225
0.19
0.1085
0.1905
0.293
0.163
0.174
0.242
0.0835
C2, R4
0.227
0.1915
0.108
0.1995
0.2955
0.1595
0.1965
0.2435
0.086
Hour 4
Hour 4:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
0.5255
0.549
0.13
0.54
0.4745
0.6265
0.5025
0.665
0.085
Colony 1, Replicate 2
0.465
0.505
0.124
0.5185
0.4115
0.4235
0.4905
0.569
0.099
Colony 1, Replicate 3
0.5155
0.5
0.126
0.51
0.4425
0.4875
0.501
0.532
0.084
Colony 1, Replicate 4
0.5115
0.5075
0.125
0.5105
0.457
0.4195
0.523
0.535
0.083
Colony 2, Replicate 1
0.5095
0.5055
0.117
0.519
0.626
0.407
0.49
0.696
0.085
Colony 2, Replicate 2
0.4635
0.4935
0.11
0.5005
0.5975
0.454
0.4475
0.562
0.085
Colony 2, Replicate 3
0.5005
0.496
0.1145
0.432
0.6055
0.4525
0.506
0.5465
0.085
Colony 2, Replicate 4
0.506
0.5
0.114
0.488
0.583
0.429
0.562
0.5665
0.084
Hour 6
Hour 6:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
Colony 1, Replicate 1
0.681
0.7495
0.198
0.7775
0.781
0.7615
0.729
0.7285
0.087
Colony 1, Replicate 2
0.6595
0.7035
0.168
0.734
0.6505
0.7015
0.7275
0.6735
0.088
Colony 1, Replicate 3
0.6635
0.7295
0.167
0.752
0.659
0.7205
0.419
0.7025
0.085
Colony 1, Replicate 4
0.655
0.724
0.162
0.708
0.727
0.6975
0.6985
0.684
0.085
Colony 2, Replicate 1
0.666
0.957
0.1645
0.6915
0.747
0.719
0.962
0.944
0.085
Colony 2, Replicate 2
0.6385
0.734
0.1295
0.6155
0.773
0.666
0.665
0.729
0.085
Colony 2, Replicate 3
0.6445
0.731
0.131
0.6445
0.754
0.611
0.6975
0.7275
0.085
Colony 2, Replicate 4
0.626
0.668
0.128
0.67
0.759
0.6395
0.6885
0.695
0.085
Fluorescence Raw Readings
These tables summarize our finalized sets of data over an incubation period of 6 hours: